發(fā)布時間：2018-10-24 15:25

【摘要】：目的探討乙醇脫氫酶(ADH)活性對絞窄性腸梗阻大鼠早期腸缺血的預測價值。 方法選取成年健康Wistar大鼠104只隨機分為對照組(A組,n=8),假手術組(B組,n=32),單純性腸梗阻(S組,n=32),絞窄性腸梗阻組(I組n=32)。A組大鼠不做任何處理；B組僅開腹翻動腸管后關腹；S組在盲腸末端以上10cm處結扎小腸；I組結扎腸系膜上動脈根部。實驗組分別于造模成功后05h、1h、3h、6h下腔靜脈取血測定血清中乙醇脫氫酶(ADH)、天冬氨酸氨基轉移酶(AST)、丙氨酸轉移酶(ALT)水平,并與肝、小腸組織蘇木精-伊紅染色后損傷病理評分做相關性分析。 結果①與A組比較,I組大鼠ADH活性在腸缺血0.5h開始升高,在6h內(nèi)未見ADH峰值出現(xiàn)。I組在0.5h、1h、3h及6h均高于B組各對應時間點ADH水平,差異有統(tǒng)計學意義(P0.05)；I組在1h、3h及6h高于S組各對應時間點ADH水平,差異有統(tǒng)計學意義(P0.05)；S組與B組各對應時間點ADH比較差異無統(tǒng)計學意義(P0.05)。與B組、S組各對應時間點比較,I組ALT、AST均在6h明顯升高,差異有顯著統(tǒng)計學意義(P0.05)；②與A組相比,I組各時間點小腸損傷評分差異均有統(tǒng)計學意義(P0.05),I組在3h、6h肝臟損傷病理評分差異有統(tǒng)計學意義(P0.05),B組、S組各時間點小腸、肝臟損傷評分差異均無統(tǒng)計學意義(P0.05)。③I組ADH、ALT、AST水平與小腸、肝臟損傷病理評分均呈正相關(r=0.696～0.795,P0.05)。 結論ADH水平在絞窄性腸梗阻早期即開始升高,與小腸損傷程度呈正相關,可能成為預測早期腸絞窄的指標。
[Abstract]:Objective to investigate the predictive value of (ADH) activity of ethanol dehydrogenase in early intestinal ischemia in rats with strangulated intestinal obstruction. Methods 104 healthy adult Wistar rats were randomly divided into three groups: control group (group A, n = 8), sham operation group (group B, n = 32), simple intestinal obstruction (group S, n = 32) and strangulated intestinal obstruction group (n = 32). A). Group S ligated the small intestine at 10cm above the caecum and group I ligated the root of superior mesenteric artery. The serum (ADH), aspartate aminotransferase (ADH),) and alanine transferase (AST),) (ALT) levels in the serum of the experimental group were measured at 0 5 h, 1 h, 3 h and 6 h, respectively, and were compared with those of the liver. The pathological score of small intestine injury after hematoxylin-eosin staining was analyzed. Results 1Compared with group A, the activity of ADH in group I began to increase at 0.5 h after intestinal ischemia, and no peak value of ADH appeared within 6 h. The level of ADH in group I was significantly higher than that in group B at 1 h and 6 h (P0.05). The level of ADH in group I was higher than that in group S at 3h and 6h, and the difference was statistically significant (P0.05). There was no significant difference in ADH between); S group and group B (P0.05). Compared with group B and group S, ALT,AST in group I increased significantly at 6h (P0.05). 2Compared with group A, the small intestine injury scores of group I were significantly different at each time point (P0.05), I group at 3 hours and 6 hours after liver injury) (P0.05), B group, S group at each time point). There was no significant difference in liver injury score (P0.05). 3in group I, there was a positive correlation between ADH,ALT,AST level and small intestine and pathological score of liver injury (r = 0.696 ~ 0.795P, P 0.05). Conclusion the level of ADH increases in the early stage of strangulated intestinal obstruction, and is positively correlated with the degree of intestinal injury, which may be a predictor of early intestinal strangulation.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R574.2

【參考文獻】

相關期刊論文 前10條

1 趙言順;馮志鵬;王驥平;王培戈;;急性腸梗阻大鼠血漿降鈣素原和C-反應蛋白變化[J];青島大學醫(yī)學院學報;2013年04期

2 石卉;吳本儼;劉文徽;蘇斌斌;李婷婷;;腸脂肪酸結合蛋白在急腹癥患者中鑒別急性腸缺血的價值[J];中華內(nèi)科雜志;2012年09期

3 李輝;王驥平;王培戈;;大鼠急性腸梗阻不同時段血漿內(nèi)毒素和D-乳酸變化[J];青島大學醫(yī)學院學報;2011年06期

4 呂云福;;腸梗阻的常見病因分類與治療策略[J];中華普外科手術學雜志(電子版);2011年03期

5 祝旭清;王國祥;;大腸癌患者血清Ⅰ型乙醇脫氫酶活性變化及意義[J];浙江中西醫(yī)結合雜志;2011年02期

6 王偉偉;何向輝;逯寧;戚峰;朱理瑋;;絞窄性腸梗阻患者血漿D-dimer的變化及臨床意義[J];中國中西醫(yī)結合外科雜志;2009年02期

7 劉偉;周曄;王影;馮虹;;降鈣素原在臨床感染方面的應用進展[J];沈陽醫(yī)學院學報;2008年03期

8 李巨華;王曉;;血清CPK、CPK-MB、P測定對絞窄性腸梗阻的診斷價值[J];吉林醫(yī)學;2007年05期

9 張志明;張才全;;不同類型腸梗阻內(nèi)毒素移位時段變化的實驗研究[J];中國普通外科雜志;2007年04期

10 孫陽,呂健宏,陳環(huán);連續(xù)監(jiān)測法測定乙醇脫氫酶及其對肝臟疾病的診斷意義[J];國外醫(yī)學(臨床生物化學與檢驗學分冊);2005年06期

相關博士學位論文 前1條

1 石卉;腸脂肪酸結合蛋白和D-乳酸在缺血性腸病診斷中的研究[D];中國人民解放軍軍醫(yī)進修學院;2011年

，

本文編號：2291762