【摘要】：目的 探討骨髓間充質(zhì)干細胞(Mesenchymal stem cells,MSCs)以及苦參素聯(lián)合MSCs對潰瘍性結(jié)腸炎(Ulcerative colitis, UC)大鼠結(jié)腸組織的修復(fù)作用。 方法 從雄性大鼠骨髓中提取MSCs。將40只雌性大鼠隨機分為正常組（A）、模型組（B）、MSCs組（C）及MSCs加苦參素組（D），每組10只。B、C、D組用三硝基苯磺酸/乙醇復(fù)合法建立UC模型，24h后，A組和B組分別經(jīng)尾靜脈輸入生理鹽水1mL；C組、D組分別經(jīng)尾靜脈輸入1×106MSC s懸液1mL；D組給予苦參素肌肉注射2周。留取結(jié)腸組織標(biāo)本，，進行病理學(xué)觀察，用PCR技術(shù)和原位熒光雜交法檢測C、D組是否存在MSCs Y染色體性別決定區(qū)域(sex determining region Y,SRY)；免疫組織化學(xué)、Western blotting檢測各組結(jié)腸組織bFGF的蛋白水平；RT-PCR技術(shù)分析結(jié)腸組織TNFα、IL-1β及bFGF mRNA的表達。 結(jié)果 C、D組結(jié)腸組織中可見SRY陽性；A組bFGF mRNA和蛋白的表達及TNFα、IL-1βmRNA的表達均較低；較A組，B、C、D組結(jié)腸組織中bFGF及TNFα、IL-1β的表達均增高(P 0.05)；較B組，C、D組bFGF表達增高，TNFα、IL-1β表達下降(P 0.05)；較C組，D組bFGF表達增高，TNFα、IL-1β表達下降(P 0.05)。 結(jié)論 MSCs能在UC大鼠結(jié)腸組織中定植，能夠抑制UC大鼠結(jié)腸組織中的炎癥反應(yīng)、修復(fù)損傷的結(jié)腸黏膜,其作用機制可能與其下調(diào)結(jié)腸組織中TNFα、IL-1β mRNA的表達、上調(diào)bFGF mRNA及蛋白的表達有關(guān)；苦參素聯(lián)合MSCs治療大鼠UC有協(xié)同增強作用。
[Abstract]:Objective to investigate the repair effect of bone marrow mesenchymal stem cells (Mesenchymal stem cells,MSCs) and matrine combined with MSCs on colonic tissue of (Ulcerative colitis, UC) rats with ulcerative colitis. Methods MSCs. was extracted from male rat bone marrow. Forty female rats were randomly divided into normal group (A), model group), (B), MSCs group (C) group) and MSCs + matrine group (D), group, 10 rats in each group). The UC model was established by trinitrobenzenesulfonic acid / ethanol compound method in group B and C group. 24 hours later, 1 mL of normal saline was injected into group A and group B respectively through tail vein. Group C and group D were injected with 1 脳 106MSC s suspension through tail vein for 2 weeks. The colonic tissue samples were collected for pathological observation, and PCR technique and in situ fluorescence hybridization were used to detect the presence of (sex determining region Y sex determining region of MSCs Y chromosome in group C ~ (2 +), and immunohistochemical, Western blotting was used to detect the protein level of bFGF in colon tissue of each group. The expression of TNF 偽, IL-1 尾 and bFGF mRNA in colon tissue was analyzed by RT-PCR technique. Results the expression of SRY, TNF 偽, IL-1 尾 mRNA, bFGF, TNF 偽 and IL-1 尾 in colon tissue of group A were lower than those in group A, and the expression of bFGF and TNF 偽, IL-1 尾 in group A were higher than those in group A (P 0.05). Compared with group B, the expression of bFGF and TNF 偽 and IL-1 尾 in group C were higher than those in group B, and the expression of bFGF and TNF 偽 and IL-1 尾 in group D were higher than those in group C (P 0.05). Conclusion MSCs can colonize the colon tissue of UC rats, inhibit the inflammatory reaction in the colon tissue of UC rats, repair the damaged colon mucosa, and its mechanism may be related to the down-regulation of the expression of TNF 偽 and IL-1 尾 mRNA in the colon tissue. The up-regulation of bFGF mRNA and protein expression was related to the synergistic effect of oxymatrine combined with MSCs on UC in rats.
【學(xué)位授予單位】：遼寧醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R574.62

【參考文獻】

相關(guān)期刊論文 前10條

1 鐘振東;蘇娟;韓麗;;苦參素對潰瘍性結(jié)腸黏膜細胞NF-κB表達的影響[J];中國醫(yī)藥科學(xué);2012年04期

2 馮亞波;姚紅;龐在英;遲兆富;;丹參注射液體外誘導(dǎo)大鼠骨髓間質(zhì)干細胞分化為神經(jīng)元樣細胞的研究[J];山東醫(yī)藥;2008年01期

3 李勇華;鄭景輝;王麗萍;簡維雄;黃獻平;袁肇凱;;養(yǎng)心通脈有效部位方動員心肌梗塞大鼠MSCs歸巢及對心功能和病理的影響[J];時珍國醫(yī)國藥;2010年09期

4 范恒;陳瑞;沈霖;呂建芳;熊鵬程;壽折星;莊雄;;Oxymatrine Improves TNBS-induced Colitis in Rats by Inhibiting the Expression of NF-κB p65[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2008年04期

5 范恒;沈霖;唐慶;熊鵬程;壽折星;廖奕;梁麗;陳小艷;;Effect of Wumeiwan on Cytokines TNF-α,IL-6,IL-8,IL-10 and Expression of NF-κBp65 in Rats with Ulcerative Colitis[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2009年05期

6 黃志新;張志堅;張陽;俞曉嵐;吳秀麗;;過表達Nanog基因的小鼠骨髓間質(zhì)干細胞對NF-κB表達的影響[J];生物工程學(xué)報;2010年05期

7 羅慶;張晨;宋關(guān)斌;;骨髓間充質(zhì)干細胞旁分泌作用在組織損傷修復(fù)中的研究進展[J];生物醫(yī)學(xué)工程學(xué)雜志;2012年05期

8 王新生;李海峰;趙熒;趙鐵軍;張曉麗;薄愛華;;黃芪誘導(dǎo)大鼠骨髓間充質(zhì)干細胞分化早期胞內(nèi)鈣調(diào)蛋白mRNA的轉(zhuǎn)錄水平[J];中國組織工程研究與臨床康復(fù);2009年23期

9 李可洲;李寧;黎介壽;鮑揚;李幼生;;肝細胞生長因子對移植小腸通透性及細菌易位的作用[J];中國修復(fù)重建外科雜志;2007年05期

10 黃獻平;袁肇凱;鄭景輝;李勇華;王麗平;簡維雄;孫貴香;王萍;黃勃;喻松仁;;養(yǎng)心通脈有效部位方對MSCs向心肌樣細胞分化的影響[J];中藥新藥與臨床藥理;2012年04期

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