【摘要】：目的 探討骨髓間充質干細胞(Mesenchymal stem cells,MSCs)以及苦參素聯合MSCs對潰瘍性結腸炎(Ulcerative colitis, UC)大鼠結腸組織的修復作用。 方法 從雄性大鼠骨髓中提取MSCs。將40只雌性大鼠隨機分為正常組（A）、模型組（B）、MSCs組（C）及MSCs加苦參素組（D），每組10只。B、C、D組用三硝基苯磺酸/乙醇復合法建立UC模型，24h后，A組和B組分別經尾靜脈輸入生理鹽水1mL；C組、D組分別經尾靜脈輸入1×106MSC s懸液1mL；D組給予苦參素肌肉注射2周。留取結腸組織標本，，進行病理學觀察，用PCR技術和原位熒光雜交法檢測C、D組是否存在MSCs Y染色體性別決定區(qū)域(sex determining region Y,SRY)；免疫組織化學、Western blotting檢測各組結腸組織bFGF的蛋白水平；RT-PCR技術分析結腸組織TNFα、IL-1β及bFGF mRNA的表達。 結果 C、D組結腸組織中可見SRY陽性；A組bFGF mRNA和蛋白的表達及TNFα、IL-1βmRNA的表達均較低；較A組，B、C、D組結腸組織中bFGF及TNFα、IL-1β的表達均增高(P 0.05)；較B組，C、D組bFGF表達增高，TNFα、IL-1β表達下降(P 0.05)；較C組，D組bFGF表達增高，TNFα、IL-1β表達下降(P 0.05)。 結論 MSCs能在UC大鼠結腸組織中定植，能夠抑制UC大鼠結腸組織中的炎癥反應、修復損傷的結腸黏膜,其作用機制可能與其下調結腸組織中TNFα、IL-1β mRNA的表達、上調bFGF mRNA及蛋白的表達有關；苦參素聯合MSCs治療大鼠UC有協(xié)同增強作用。
[Abstract]:Objective to investigate the repair effect of bone marrow mesenchymal stem cells (Mesenchymal stem cells,MSCs) and matrine combined with MSCs on colonic tissue of (Ulcerative colitis, UC) rats with ulcerative colitis. Methods MSCs. was extracted from male rat bone marrow. Forty female rats were randomly divided into normal group (A), model group), (B), MSCs group (C) group) and MSCs + matrine group (D), group, 10 rats in each group). The UC model was established by trinitrobenzenesulfonic acid / ethanol compound method in group B and C group. 24 hours later, 1 mL of normal saline was injected into group A and group B respectively through tail vein. Group C and group D were injected with 1 脳 106MSC s suspension through tail vein for 2 weeks. The colonic tissue samples were collected for pathological observation, and PCR technique and in situ fluorescence hybridization were used to detect the presence of (sex determining region Y sex determining region of MSCs Y chromosome in group C ~ (2 +), and immunohistochemical, Western blotting was used to detect the protein level of bFGF in colon tissue of each group. The expression of TNF 偽, IL-1 尾 and bFGF mRNA in colon tissue was analyzed by RT-PCR technique. Results the expression of SRY, TNF 偽, IL-1 尾 mRNA, bFGF, TNF 偽 and IL-1 尾 in colon tissue of group A were lower than those in group A, and the expression of bFGF and TNF 偽, IL-1 尾 in group A were higher than those in group A (P 0.05). Compared with group B, the expression of bFGF and TNF 偽 and IL-1 尾 in group C were higher than those in group B, and the expression of bFGF and TNF 偽 and IL-1 尾 in group D were higher than those in group C (P 0.05). Conclusion MSCs can colonize the colon tissue of UC rats, inhibit the inflammatory reaction in the colon tissue of UC rats, repair the damaged colon mucosa, and its mechanism may be related to the down-regulation of the expression of TNF 偽 and IL-1 尾 mRNA in the colon tissue. The up-regulation of bFGF mRNA and protein expression was related to the synergistic effect of oxymatrine combined with MSCs on UC in rats.
【學位授予單位】：遼寧醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R574.62

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