【摘要】：目的:觀察龍血竭聯(lián)合魚腥草對UC模型大鼠疾病活動指數(shù)、結(jié)腸黏膜大體形態(tài)、病理組織學(xué)改變,并檢測UC模型大鼠血清超氧化物歧化酶(SOD)與丙二醛(MDA)的變化,驗證魚腥草及龍血竭對UC的治療作用,探討二者聯(lián)合應(yīng)用是否對UC的治療有協(xié)同作用及其作用機(jī)理,從而為UC規(guī)范化治療及用藥提供依據(jù)。方法:將SD大鼠隨機(jī)分為正常對照組和模型組。模型組予以2,4,6-三硝基苯磺酸加乙醇灌腸造模。當(dāng)UC模型造模成功后,將模型組大鼠隨機(jī)分為龍血竭組,魚腥草組,龍血竭合魚腥草組低、中、高劑量組,柳氮磺吡啶(SASP)組及模型對照組。分別給藥灌胃兩周后,心臟采血進(jìn)行血清SOD、MDA檢測,剖腹留取病變結(jié)腸做病理切片。結(jié)果:藥物灌胃后,各灌胃組大鼠體重均有增加,龍血竭合魚腥草高、中劑量組兩者之間比較,差異無統(tǒng)計學(xué)意義(P0.05);龍血竭合魚腥草高、中劑量組與SASP組、龍血竭組、魚腥草組相比,體重增加量更大,差異具有統(tǒng)計學(xué)意義(P0.05)。各灌胃組大鼠結(jié)腸粘膜充血水腫,糜爛及潰瘍等癥狀均有改善,龍血竭合魚腥草高、中劑量組、龍血竭組、魚腥草組四組之間比較,差異無統(tǒng)計學(xué)意義(P0.05);龍血竭合魚腥草高、中劑量組、龍血竭組、魚腥草組與SASP組相比,結(jié)腸黏膜損傷指數(shù)更低,差異具有統(tǒng)計學(xué)意義(P0.05)。各灌胃組血清中SOD濃度均有升高,血清中MDA濃度均有降低,在幅度上龍血竭合魚腥草高、中劑量組與SASP組、魚腥草組、龍血竭組相比,差異具有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:本實驗研究表明龍血竭合魚腥草高、中劑量組能夠改善結(jié)腸粘膜充血水腫、糜爛出血、潰瘍等癥狀,提高血清SOD活性、降低MDA含量,且優(yōu)于SASP組、魚腥草組、龍血竭組,提示龍血竭合魚腥草中、高劑量組對UC大鼠有較好的療效。其作用機(jī)理之一可能是通過增強(qiáng)機(jī)體抗氧化能力、抑制組織中的脂質(zhì)過氧化反應(yīng),穩(wěn)定細(xì)胞膜、從而保護(hù)腸粘膜、促進(jìn)潰瘍面愈合,達(dá)到治療UC的作用。
[Abstract]:Objective: to observe the changes of disease activity index, colonic mucosa gross morphology and histopathology in UC model rats treated with Draconis and Houttuynia houttuyniae, and to detect the changes of serum superoxide dismutase (SOD) and malondialdehyde (MDA) (MDA) in UC model rats. To verify the therapeutic effect of Houttuynia cordata and Dragon Blood Draconis on UC, and to explore whether the combined use of Houttuynia cordata and Draconis has synergistic effect on UC and its mechanism, so as to provide the basis for the standardized treatment and medication of UC. Methods: SD rats were randomly divided into normal control group and model group. The model group was made by enema with 2: 4 and 6-trinitrobenzene sulfonic acid plus ethanol. When the UC model was successfully established, the rats in the model group were randomly divided into four groups: Dragon blood group, houttuynia group, low, middle and high dose group, (SASP) group and model control group. After two weeks of administration, the blood samples were collected from the heart to detect the serum levels of SOD and MDA, and the pathological colon was taken by laparotomy for pathological sections. Results: after intragastric administration, the body weight of rats in each gavage group was increased, and the body weight of rats in each group was higher than that in the middle dose group (P0.05), and there was no significant difference between the two groups (P0.05), while in the middle dose group and SASP group, there was no significant difference between them (P0.05), while in the middle dose group and SASP group, there was no significant difference between them (P0.05). Compared with Houttuynia cordata group, the weight gain was greater, the difference was statistically significant (P0.05). The hyperemia, edema, erosion and ulceration of colonic mucosa of rats in each group were improved, and there was no significant difference among the four groups (P0.05), and there was no significant difference among the four groups (P0.05), and there was no significant difference among the four groups (P 0.05), and there was no significant difference among the four groups, such as the combination of dragon blood and houttuynia houttuyniae. Compared with SASP group, the colon mucosal injury index of middle dose group, Dragon blood group and Houttuynia group was lower, the difference was statistically significant (P0.05). The concentration of SOD in serum was increased and the concentration of MDA in serum was decreased in each group. There was significant difference between middle dose group and SASP group, houttuynia group and dragon blood group in the range of Draconis houttuyniae and Houttuynia houttuyniae (P0.05). Conclusion: the experimental study shows that Dragon Blood Draconis combined with Houttuynia Houttuyniae is high. The middle dose group can improve the symptoms of congestion and edema, erosive bleeding, ulcer and other symptoms of colon mucosa, increase the activity of serum SOD and decrease the content of MDA, and it is superior to the SASP group, Houttuynia group and Dragon blood group. It was suggested that the high dose group had better curative effect on UC rats in the combination of Dragon Blood and Houttuynia cordata. One of its mechanisms may be to enhance the antioxidant ability of the body, inhibit lipid peroxidation in tissues, stabilize cell membrane, protect intestinal mucosa, promote ulcer healing, and achieve the effect of treating UC.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R574.62

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