本文選題：肝臟儲備功能 + 分光光度技術(shù)��； 參考：《山東醫(yī)藥》2015年04期

【摘要】：目的建立基于分光光度法測定非那西丁與撲熱息痛含量的肝臟儲備功能評估方法。方法篩選分光光度測定的顯色體系,確定最大吸收波長,分析不同因素對顯色體系的影響,優(yōu)化非那西丁與撲熱息痛水解的最佳條件,最后對技術(shù)體系進行應(yīng)用性驗證。結(jié)果建立了利用分光光度測定樣品中非那西丁和撲熱息痛含量的技術(shù)體系,即樣品加入3 mol/L鹽酸水解30 min,加入0.02%1,2-萘醌-4-磺酸鈉、1%十六烷基三甲基溴化銨及2%Na OH(或3%Na2CO3)(比例為1∶6∶1∶2或3),分別于500 nm和570 nm下測定吸光值,計算各自濃度。該技術(shù)測定血漿樣品中非那西丁與撲熱息痛時的分辨率和重復(fù)性與高效液相色譜法相當。結(jié)論利用分光光度技術(shù)測定血液中非那西丁與撲熱息痛含量,二者比值具有評估肝臟儲備功能價值。
[Abstract]:Objective to establish a method for evaluating liver reserve function based on spectrophotometric determination of phenacetin and paracetamol. Methods the color reaction system for spectrophotometric determination was screened, the maximum absorption wavelength was determined, the influence of different factors on the color reaction system was analyzed, the optimum conditions for the hydrolysis of phenacetin and paracetamol were optimized, and the application of the technical system was verified. Results A technical system for the determination of phenacetin and paracetamol in samples by spectrophotometry was established. That is to say, the sample was hydrolyzed with 3 mol / L hydrochloric acid for 30 min, and the absorptivity was determined at 500nm and 570nm, respectively, by adding 0.021ka-2-naphthoquinone-4-sulfonate sodium 1% hexadecyltrimethylammonium bromide and 2sodium hydroxide (or 3Na2CO3) (1: 6: 1: 2 or 3). The absorptivity was determined at 500nm and 570nm, respectively. The resolution and repeatability of determination of phenacetin and paracetamol in plasma samples by this technique are comparable to those of HPLC. Conclusion the content of phenacetin and paracetamol in blood was determined by spectrophotometry. The ratio of phenacetin and paracetamol has the value of evaluating liver reserve function.
【作者單位】： 山東省千佛山醫(yī)院;
【基金】：山東省科技攻關(guān)項目(2009GG10002005) 山東省醫(yī)藥衛(wèi)生科研項目(2007HW116)
【分類號】：R575

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本文編號：2095355