本文選題：潰瘍性結(jié)腸炎 + IL-12B��； 參考：《青島大學》2017年碩士論文

【摘要】：研究背景:潰瘍性結(jié)腸炎(ulcerative colitis-UC)是炎癥性腸病的一種,為非特異性慢性炎癥性疾病,目前病因尚未明確。雖然目前一些無創(chuàng)、方便的生物標志物檢測UC具有一定價值,但敏感性和特異性有限,亦取代不了內(nèi)鏡評價的需要。內(nèi)鏡因其經(jīng)濟因素及操作的風險性,尚不能成為潰瘍性結(jié)腸炎的普查手段。有研究表明血清IL-12B的表達與UC的活動性具有相關性,血清IL-12B有望成為預測UC活動性及預后的有效標志物。研究目的:測定UC患者臨床及內(nèi)鏡下不同活動期的血清IL-12B的表達水平,并進行比較,確定血清IL-12B表達與UC活動性及其黏膜愈合的相關性,評價血清IL-12B作為一種診斷潰瘍性結(jié)腸炎的生物標記物的可能性。研究方法:收集80例潰瘍性結(jié)腸炎住院患者及20例健康對照者的臨床信息、血清及結(jié)腸鏡檢查結(jié)果,均來自青島市市立醫(yī)院,血清IL-12B濃度采用酶聯(lián)免疫吸附法(ELISA)測定,潰瘍性結(jié)腸炎臨床活動度由炎癥性腸病Montreal分類評估,潰瘍性結(jié)腸炎內(nèi)鏡下疾病活動度以Mayo內(nèi)鏡評分為標準。并將潰瘍性結(jié)腸炎患者臨床和內(nèi)鏡下血清IL-12B濃度聯(lián)合進行評估。P0.05認為有統(tǒng)計學意義。研究結(jié)果:潰瘍性結(jié)腸炎患者血清IL-12B水平明顯高于對照組(2.75pg/ml,1.43pg/ml,P0.05)。臨床活動期明顯高于臨床緩解期(3.0pg/ml,1.05pg/ml,P0.05),差異有統(tǒng)計學意義;內(nèi)鏡下活動期高于緩解期(1.88pg/ml,1.74pg/ml,P0.05),差異有統(tǒng)計學意義;疾病變化病程中IL-12B水平變化采用配對t檢驗,Spearman相關分析分別檢測IL-12B表達水平與臨床及內(nèi)鏡下疾病嚴重程度,均呈正相關(r=0.90,r=0.95,P0.05)。研究結(jié)論:血清IL-12B水平與潰瘍性結(jié)腸炎疾病活動性及黏膜愈合具有相關性(P0.05),血清IL-12B水平隨潰瘍性結(jié)腸炎嚴重程度增加而增加(P0.05)。表明血清IL-12B可以成為UC患者疾病活動性及黏膜愈合評估的潛在生物標志物。
[Abstract]:Background: ulcerative colitis-UCis is a kind of inflammatory bowel disease, which is nonspecific chronic inflammatory disease. Although some noninvasive and convenient biomarker detection of UC has some value, but the sensitivity and specificity is limited, it can not replace the need of endoscopic evaluation. Because of its economic factors and operational risk, endoscopy can not be used as a general method for ulcerative colitis. Some studies have shown that the expression of serum IL-12B is correlated with the activity of UC, and serum IL-12B may be an effective marker for predicting UC activity and prognosis. Objective: to determine the relationship between serum IL-12B expression and UC activity and mucosal healing in patients with UC. To evaluate the possibility of serum IL-12 B as a biomarker for diagnosis of ulcerative colitis. Methods: the clinical information of 80 patients with ulcerative colitis and 20 healthy controls were collected. The results of serum and colonoscopy were collected from Qingdao City Hospital. The serum IL-12B concentration was determined by enzyme linked immunosorbent assay (Elisa). The clinical activity of ulcerative colitis was evaluated by Montreal classification of inflammatory bowel disease. The clinical and endoscopic serum IL-12B levels in patients with ulcerative colitis were evaluated. Results: the serum levels of IL-12B in patients with ulcerative colitis were significantly higher than those in the control group (2.75 PG / ml 1.43 PG / ml P 0.05). The difference was statistically significant in the active stage than that in the remission stage (3.0pg / ml, 1.05pg / ml, P 0.05N), and in the active stage under endoscopy was 1.88pg / ml 1.74pg / ml, the difference was statistically significant, compared with that in the remission stage (1.88pg / ml, 1.74pg / ml, P 0.05g / L). The expression of IL-12B was detected by paired t-test and Spearman correlation analysis. The expression of IL-12B was positively correlated with the clinical and endoscopic disease severity. Conclusion: the level of serum IL-12B is correlated with the activity of ulcerative colitis and mucosal healing. The level of serum IL-12B increases with the severity of ulcerative colitis. Serum IL-12 B may be a potential biomarker for the assessment of disease activity and mucosal healing in UC patients.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R574.62

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