發(fā)布時(shí)間：2018-06-12 07:26

  本文選題：潰瘍性結(jié)腸炎 + IL-12B�。� 參考：《青島大學(xué)》2017年碩士論文

【摘要】：研究背景:潰瘍性結(jié)腸炎(ulcerative colitis-UC)是炎癥性腸病的一種,為非特異性慢性炎癥性疾病,目前病因尚未明確。雖然目前一些無創(chuàng)、方便的生物標(biāo)志物檢測(cè)UC具有一定價(jià)值,但敏感性和特異性有限,亦取代不了內(nèi)鏡評(píng)價(jià)的需要。內(nèi)鏡因其經(jīng)濟(jì)因素及操作的風(fēng)險(xiǎn)性,尚不能成為潰瘍性結(jié)腸炎的普查手段。有研究表明血清IL-12B的表達(dá)與UC的活動(dòng)性具有相關(guān)性,血清IL-12B有望成為預(yù)測(cè)UC活動(dòng)性及預(yù)后的有效標(biāo)志物。研究目的:測(cè)定UC患者臨床及內(nèi)鏡下不同活動(dòng)期的血清IL-12B的表達(dá)水平,并進(jìn)行比較,確定血清IL-12B表達(dá)與UC活動(dòng)性及其黏膜愈合的相關(guān)性,評(píng)價(jià)血清IL-12B作為一種診斷潰瘍性結(jié)腸炎的生物標(biāo)記物的可能性。研究方法:收集80例潰瘍性結(jié)腸炎住院患者及20例健康對(duì)照者的臨床信息、血清及結(jié)腸鏡檢查結(jié)果,均來自青島市市立醫(yī)院,血清IL-12B濃度采用酶聯(lián)免疫吸附法(ELISA)測(cè)定,潰瘍性結(jié)腸炎臨床活動(dòng)度由炎癥性腸病Montreal分類評(píng)估,潰瘍性結(jié)腸炎內(nèi)鏡下疾病活動(dòng)度以Mayo內(nèi)鏡評(píng)分為標(biāo)準(zhǔn)。并將潰瘍性結(jié)腸炎患者臨床和內(nèi)鏡下血清IL-12B濃度聯(lián)合進(jìn)行評(píng)估。P0.05認(rèn)為有統(tǒng)計(jì)學(xué)意義。研究結(jié)果:潰瘍性結(jié)腸炎患者血清IL-12B水平明顯高于對(duì)照組(2.75pg/ml,1.43pg/ml,P0.05)。臨床活動(dòng)期明顯高于臨床緩解期(3.0pg/ml,1.05pg/ml,P0.05),差異有統(tǒng)計(jì)學(xué)意義;內(nèi)鏡下活動(dòng)期高于緩解期(1.88pg/ml,1.74pg/ml,P0.05),差異有統(tǒng)計(jì)學(xué)意義;疾病變化病程中IL-12B水平變化采用配對(duì)t檢驗(yàn),Spearman相關(guān)分析分別檢測(cè)IL-12B表達(dá)水平與臨床及內(nèi)鏡下疾病嚴(yán)重程度,均呈正相關(guān)(r=0.90,r=0.95,P0.05)。研究結(jié)論:血清IL-12B水平與潰瘍性結(jié)腸炎疾病活動(dòng)性及黏膜愈合具有相關(guān)性(P0.05),血清IL-12B水平隨潰瘍性結(jié)腸炎嚴(yán)重程度增加而增加(P0.05)。表明血清IL-12B可以成為UC患者疾病活動(dòng)性及黏膜愈合評(píng)估的潛在生物標(biāo)志物。
[Abstract]:Background: ulcerative colitis-UCis is a kind of inflammatory bowel disease, which is nonspecific chronic inflammatory disease. Although some noninvasive and convenient biomarker detection of UC has some value, but the sensitivity and specificity is limited, it can not replace the need of endoscopic evaluation. Because of its economic factors and operational risk, endoscopy can not be used as a general method for ulcerative colitis. Some studies have shown that the expression of serum IL-12B is correlated with the activity of UC, and serum IL-12B may be an effective marker for predicting UC activity and prognosis. Objective: to determine the relationship between serum IL-12B expression and UC activity and mucosal healing in patients with UC. To evaluate the possibility of serum IL-12 B as a biomarker for diagnosis of ulcerative colitis. Methods: the clinical information of 80 patients with ulcerative colitis and 20 healthy controls were collected. The results of serum and colonoscopy were collected from Qingdao City Hospital. The serum IL-12B concentration was determined by enzyme linked immunosorbent assay (Elisa). The clinical activity of ulcerative colitis was evaluated by Montreal classification of inflammatory bowel disease. The clinical and endoscopic serum IL-12B levels in patients with ulcerative colitis were evaluated. Results: the serum levels of IL-12B in patients with ulcerative colitis were significantly higher than those in the control group (2.75 PG / ml 1.43 PG / ml P 0.05). The difference was statistically significant in the active stage than that in the remission stage (3.0pg / ml, 1.05pg / ml, P 0.05N), and in the active stage under endoscopy was 1.88pg / ml 1.74pg / ml, the difference was statistically significant, compared with that in the remission stage (1.88pg / ml, 1.74pg / ml, P 0.05g / L). The expression of IL-12B was detected by paired t-test and Spearman correlation analysis. The expression of IL-12B was positively correlated with the clinical and endoscopic disease severity. Conclusion: the level of serum IL-12B is correlated with the activity of ulcerative colitis and mucosal healing. The level of serum IL-12B increases with the severity of ulcerative colitis. Serum IL-12 B may be a potential biomarker for the assessment of disease activity and mucosal healing in UC patients.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R574.62

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張玲玲;許鐘;黃國(guó)美;白班俊;梁麗;李宇;;血清抗體檢測(cè)在炎癥性腸病中的臨床應(yīng)用研究[J];中國(guó)實(shí)用醫(yī)藥;2016年26期

2 王璋;吳泉峰;;血清自身抗體P-ANCA及GAB檢測(cè)在潰瘍性結(jié)腸炎中的臨床診斷價(jià)值探討[J];檢驗(yàn)醫(yī)學(xué)與臨床;2016年16期

3 袁柏思;金鑫鑫;路又可;劉炯;王震凱;吳琳;汪芳裕;;檢測(cè)6種血清學(xué)抗體在120例炎癥性腸病中的臨床應(yīng)用價(jià)值[J];中華消化雜志;2015年04期

4 黃麗娟;高敏;曹倩;;血清抗釀酒酵母菌抗體檢測(cè)對(duì)克羅恩病的臨床價(jià)值[J];中華消化雜志;2014年10期

5 楊梅;許翠萍;李倩;;抗中性粒細(xì)胞和釀酒酵母細(xì)胞抗體測(cè)定在炎癥性腸病中的應(yīng)用[J];當(dāng)代醫(yī)學(xué);2014年11期

6 李曉軍;劉陽;;炎癥性腸病的血清標(biāo)志物研究進(jìn)展[J];臨床檢驗(yàn)雜志;2014年03期

7 謝文;汪付兵;余曉萍;龍行華;楊桂;李瑞炎;;潰瘍性結(jié)腸炎患者血清自身抗體檢測(cè)的臨床價(jià)值[J];國(guó)際檢驗(yàn)醫(yī)學(xué)雜志;2013年15期

8 李慕然;劉艷迪;鄭曉莉;張慶瑜;;血清學(xué)抗體檢測(cè)對(duì)炎癥性腸病的診斷價(jià)值[J];中華臨床醫(yī)師雜志(電子版);2013年06期

9 梁潔;周林;沙素梅;雷少妮;羅貫虹;吳開春;;炎癥性腸病診斷與治療的共識(shí)意見(2012年·廣州)潰瘍性結(jié)腸炎診斷部分解讀[J];胃腸病學(xué);2012年12期

10 李文克;李文倩;馮建明;艾國(guó);;多發(fā)性骨髓瘤不同分期C-反應(yīng)蛋白、血紅蛋白、紅細(xì)胞沉降率檢測(cè)的臨床意義[J];白血病.淋巴瘤;2012年03期

，

本文編號(hào)：2008872