本文關鍵詞：柴芍胃炎顆粒對功能性消化不良模型大鼠胃竇Cajal間質(zhì)細胞表達的影響，由筆耕文化傳播整理發(fā)布。

        目的:建立大鼠功能性消化不良模型，通過柴芍胃炎顆粒對功能性消化不良(functional dyspepsia FD)模型大鼠療效觀察及胃竇部Cajal間質(zhì)細胞（Interstitial cells of Cajal ICC）表達的影響，探討柴芍胃炎顆粒治療FD的作用機制。方法:將60只SD大鼠按隨機化原則分成6組：空白對照組、模型對照組、柴芍胃炎顆粒高、中、低劑量組、多潘立酮組，每組10只。分籠飼養(yǎng)于鋼絲網(wǎng)底塑料籠內(nèi)，每籠5只，每組2籠。將同籠老鼠使用“夾尾刺激法”和“不規(guī)則喂養(yǎng)”造模14天，誘導FD的動物模型，造模期間觀察大鼠飲水量和飲食量的變化。造模結束后用生理鹽水、柴芍胃炎顆粒、多潘立酮干預FD模型大鼠，觀察飲水量和飲食量的變化，14天后，采取腹主動脈取血觀察大鼠血漿胃動素(Motilin MTL)、血清胃泌素(Gastrin GAS)水平，檢測胃排空率，用免疫組化法檢測c-kit，觀察ICC的數(shù)量變化，以探討柴芍胃炎顆粒治療FD的作用機理。結果：1.一般情況的觀察：造模結束后，空白對照組大鼠飲水、飲食量較穩(wěn)定，無明顯變化，體重較造模前明顯增加；毛色潔白、柔軟，有光澤，糞便性狀、量和氣味無特殊改變；精神狀態(tài)良好。模型對照組大鼠飲水、飲食量逐漸減少，體重呈下降趨勢；毛發(fā)污黃，干澀無光澤，糞便質(zhì)軟，含水份較多，氣味穢臭刺鼻；性情暴躁，易被激惹，甚至相互撕咬，易驚恐，并互相聚集于鼠籠一角；中藥組、多潘立酮組：大鼠治療前一般情況與模型對照組大鼠無明顯差別，治療后，進食量、飲水量，體重較前明顯增加；毛色無明顯改變；糞便干燥含水量減少，氣味穢臭；性情稍溫順，活潑，撕打明顯減少。2、解剖大鼠觀察胃及十二指腸有無器質(zhì)性病變：造模結束后，6個實驗組中，各組隨機取大鼠2只，行腹部解剖剪開胃、十二指腸觀察到胃及十二指腸粘膜色澤正常，無充血水腫，糜爛，，出血，無潰瘍、隆起等器質(zhì)性病變，符合FD的鏡下特征。3、各組大鼠日均飲水量的比較：①與空白對照組比較：模型對照組、低、中、高劑量組、多潘立酮組日均飲水量明顯低于空白對照組（P<0.01）。②與模型對照組比較：低、中、高劑量組、多潘立酮組日均飲水量明顯高于模型對照組（P<0.01）。③與低劑量組比較：中、高劑量組、多潘立酮組日均飲水量明顯高于低劑量組（P<0.01）。④與中劑量組比較：高劑量組日均飲水量明顯高于中劑量組，多潘立酮組日均飲水量明顯小于中劑量組（P<0.01）。⑤與高劑量組比較：多潘立酮組日均飲水量明顯低于高劑量組（P<0.01）。4、各組大鼠日均飲食量的比較：①與空白對照組比較：模型對照組、低、中、高劑量組、多潘立酮組日均飲食量明顯低于空白對照組（P<0.01）。②與模型對照組比較：低、中、高劑量組、多潘立酮組日均飲食量明顯高于模型對照組（P<0.01）。③與低劑量組比較：中、高劑量組、多潘立酮組日均飲食量明顯高于低劑量組（P<0.01）。④與中劑量組比較：高劑量組、多潘立酮組日均飲食量明顯高于中劑量組（P<0.01）。⑤與高劑量組比較：多潘立酮組日均飲食量明顯低于高劑量組（P<0.01）。5、各組大鼠體重的比較：①與空白對照組比較：模型對照組、低、中、高劑量組、多潘立酮組體重明顯低于空白對照組（P<0.01）。②與模型對照組比較：高劑量組、多潘立酮組體重明顯高于模型對照組（P<0.01）。③與低劑量組比較：高劑量組、多潘立酮組體重明顯高于低劑量組（P<0.01）。④與中劑量組比較：高劑量組、多潘立酮組體重明顯高于中劑量組（P<0.01）。⑤與高劑量組比較：多潘立酮組體重明顯低于高劑量組（P<0.01）。6、各組大鼠胃排空率的比較：①與空白對照組比較：模型組、低、中劑量組、多潘立酮組胃排空率明顯低于空白對照組（P<0.01），高劑量組與空白對照組胃排空率比較無統(tǒng)計學意義（P>0.05）。②與模型對照組比較：低、中、高劑量組、多潘立酮組胃排空率明顯高于模型對照組（P<0.01）。③與低劑量組比較：中、高劑量組、多潘立酮組胃排空率明顯高于低劑量組（P<0.01）。④與中劑量組比較：高劑量組、多潘立酮組胃排空率明顯高于中劑量組（P<0.01）。⑤與高劑量組比較：多潘立酮組胃排空率明顯低于高劑量組（P<0.01）。7、各組大鼠胃動素（MTL）的比較：①與空白對照組比較：模型對照組胃動素明顯低于空白對照組（P<0.01），高劑量組胃動素明顯高于空白對照組（P<0.01）。②與模型對照組比較：低、中、高劑量組、多潘立酮組胃動素明顯高于模型對照組（P<0.01）。③與低劑量組比較：高劑量組胃動素明顯高于低劑量組（P<0.01），中劑量組、多潘立酮組與低劑量組胃動素比較無統(tǒng)計學意義（P>0.05）。④與中劑量組比較：高劑量組、多潘立酮組與中劑量組胃動素比較無統(tǒng)計學意義（P>0.05）。⑤與高劑量組比較：多潘立酮組明顯低于高劑量組（P<0.01）。8、各組大鼠胃泌素（GAS）的比較：①與空白對照組比較：模型對照組、低、中劑量組、多潘立酮組胃泌素明顯低于空白對照組（P<0.01），高劑量組與空白組胃泌素比較無統(tǒng)計學意義（P>0.05）。②與模型對照組比較：低、中、高劑量組、多潘立酮組胃泌素明顯高于模型對照組（P<0.01）。③與低劑量組比較：高劑量組胃泌素高于低劑量組（P<0.05），中劑量組、多潘立酮組與低劑量組胃泌素比較無統(tǒng)計學意義（P>0.05）。④與中劑量組比較：高劑量組胃泌素高于中劑量組（P<0.05），多潘立酮組與中劑量組胃泌素比較無統(tǒng)計學意義（P>0.05）；⑤與高劑量組比較：多潘立酮組胃泌素明顯低于高劑量組（P<0.01）。9、各組大鼠胃竇組織中ICC陽性細胞的灰度值的比較：①與空白對照組比較：模型對照組、低、中、高劑量組、多潘立酮組胃竇ICC陽性細胞灰度值明顯低于空白對照組（P<0.01）。②與模型對照組比較：低、中、高劑量組、多潘立酮組胃竇ICC陽性細胞灰度值明顯高于模型對照組（P<0.01）。③與低劑量組比較：高劑量組胃竇ICC陽性細胞灰度值高于低劑量組（P<0.05），中劑量組、多潘立酮組胃竇ICC陽性細胞灰度值與低劑量組比較無統(tǒng)計學意義（P>0.05）。④與中劑量組比較：高劑量組胃竇ICC陽性細胞灰度值高于中劑量組（P<0.05），多潘立酮組胃竇ICC陽性細胞灰度值與中劑量組比較無統(tǒng)計學意義（P>0.05）。⑤與高劑量組比較：多潘立酮組胃竇ICC陽性細胞灰度值明顯低于高劑量組（P<0.01）。結論:1、應用“夾尾刺激法”和“不規(guī)則喂養(yǎng)”聯(lián)合造模，是單一采用“夾尾刺激法”或“不規(guī)則喂養(yǎng)”制備FD大鼠模型的改良，能復制較理想的FD大鼠模型。2、柴芍胃炎顆粒高劑量組胃竇ICC陽性細胞灰度值高于模型對照組、低、中劑量組、多潘立酮組，這說明高劑量組增加胃竇ICC的數(shù)量較模型對照組、低、中劑量組、多潘立酮組好。3、柴芍胃炎顆粒高劑量組對FD大鼠模型胃動素、胃泌素、胃排空率的影響優(yōu)于模型對照組、低、中劑量組、多潘立酮組，這說明高劑量組能較好的促進胃腸動力。4、柴芍胃炎顆粒治療FD大鼠的可能機制是：其影響了FD大鼠胃竇中的ICC，增加了胃竇ICC的數(shù)量，從而使胃動素、胃泌素的分泌增加，增強了胃腸道的動力，從而促進了胃排空。

    Objective： To establish on rats model of functionaldyspepsia,study the action mechanism of functional dyspepsia withChaishao gastritis particle therapy by observing on efficacy of Chaishaogastritis particles of functional dyspepsia rat model, and affecting onexpression of interstitial cells of Cajal in the rat antrum of Chaishaogastritis particles to functional dyspepsia model. Methods:60SD ratswere divided into6groups by the principle of randomization: blankcontrol group, model control group, high dose group Chaishao gastritisparticles, middle dose group Chaishao gastritis particles, low does groupChaishao gastritis particles,domperidone group (n=10). At the end ofsteel mesh plastic of cage,five per cage (n=2cage), feeding ratsseparately. The same cage of rats using the clip tail stimulation and madeirregular feeding mode14days,induced animal models of functionaldyspepsia modeling,rats were observed during the water intake and foodintake changes. After the modeling,with saline,Chaishao gastritis particles,domperidone interven the functional dyspepsia rat model to observechanges in water intake and food intake.14days later, to take theabdominal aorta blood to observe in rat plasma motilin levels, serumgastrin level, detection of gastric emptying rate,observed c-kit with immun ohistochemical assay, the ICC number of changes. To explore themechanism of functional dyspepsia with Chaishao gastritis particletherapies. Results:1.General observation: after the end of modeling, theblank control rats, water intake and food intake is more stable, nosignificant changes,the body weight compared with before modelingsignificantly increased; hair white, soft, luster, stool volume and odor nospecial change; good state of spirit. Model control rats, water intake andfood intake is gradually reduced, the weight gradually reduced trend;dirty yellow hair dry and dull, stool soft, more moisture, full of smellpungent; irascible, susceptible to irritation, and even biting each other,easily frightened, and each other gathered in the squirrel cage corner of;Chaishao gastritis particle group, domperidone group: before therapy, ratsgeneral situation with the model group rats no significant difference, aftertherapy, food intake, water intake, body weight significantly increasedcompared with the previous; hair no obvious change; Fecal dry，moisturecontent to reduce,full of the smell; temperament slightly docile,livelytussle significantly reduced.2. To observe rats with anatomy of have orwithout organic lesions of the stomach and duodenum: after the end ofmodel,the six experimental groups, each group were randomly selectedrats2, cut appetizer and duodenum,no observed stomach and duodenalmucosa, congestion and edema, erosion, bleeding, ulcer, uplift, and otherorganic disease, conform with endoscopic features of functional dyspepsia.3. Comparision of average daily water intake of rats in eachgroup:①Compared with the blank control group: average daily waterintake of model group, low dose group, middle dose group,high dosegroup and domperidone group was significantly lower than the blankcontrol group（P<0.01).②Compared with the model control group:average daily water intake of low dose group, middle dose group, highdose group and the group domperidone was significantly higher than themodel control group （P<0.01).③Compared with the low dosegroup:average daily water intake of middle dose group, high dose groupand the group domperidone was significantly higher than the low dosegroup（P<0.01).④Compared with the middle dose group:average dailywater intake of high dose group was significantly higher than themiddle dose group,the group domperidone was significantly lower thanthe middle dose group（P<0.01).⑤Compared with the high dosegroup:average daily water intake of the group domperidone wassignificantly lower than the high dose group（P<0.01).4. Comparisionof average daily food intake of rats in each group:①Compared with theblank control group: average daily food intake of model group, low dosegroup, middle dose group,high dose group and domperidone group wassignificantly lower than the blank control group（P<0.01).②Comparedwith the model control group: average daily food intake of low dosegroup, middle dose group, high dose group and the group domperidone was significantly higher than the model control group（P<0.01).③Compared with the low dose group:average daily food intake of middledose group, high dose group and the group domperidone wassignificantly higher than the low dose group（P<0.01).④Compared withthe middle dose group:average daily food intake of high dose group andthe group domperidone was significantly higher than the middle dosegroup（P<0.01).⑤Compared with the high dose group:average dailyfood intake of the group domperidone was significantly lower than thehigh dose group（P<0.01).5.Comparision of weight of rats in eachgroup:①Compared with the blank control group:weight of model group,low dose group, middle dose group,high dose group and domperidonegroup was significantly lower than the blank control group（P<0.01).②Compared with the model control group: weight of high dose group anddomperidone group was significantly higher than the model group（P<0.01).③Compared with the low dose group:weight of high dosegroup and domperidone group was significantly higher than the low dosegroup（P<0.01).④Compared with the middle dose group:weight of highdose group and the group domperidone was significantly higher than themiddle dose group （P<0.01).⑤Compared with the high dosegroup:weight of the group domperidone was significantly lower than thehigh dose group（P<0.01).6.Comparision of gastric emptying rate ofrats in each group:①Compared with the blank control group: gastric emptying rate of model group,low dose group, middle dose group anddomperidone group was significantly lower than the blank control group（P<0.01).the high dose group and the blank control group of gastricemptying rate was no significant difference（P>0.05）.②Compared withthe model control group: gastric emptying rate of low dose group, middledose group, high dose group and the group domperidone wassignificantly higher than the model control group（P<0.01).③Comparedwith the low dose group:gastric emptying rate of middle dose group, highdose group and the group domperidone was significantly higher than thelow dose group （P<0.01).④Compared with the middle dosegroup:gastric emptying rate of high dose group and the groupdomperidone was significantly higher than the middle dose group（P<0.01).⑤Compared with the high dose group:gastric emptying rateof the group domperidone was significantly lower than the high dosegroup（P<0.01).7.Comparision of Motilin of rats in each group:①Compared with the blank control group: Motilin of model group wassignificantly lower than the blank control group（P<0.01),Motilin ofhigh dose group significantly higher than the blank control group（P<0.01).②Compared with the model control group: Motilin of lowdose group, middle dose group, high dose group and the groupdomperidone was significantly higher than the model control group（P<0.01).③Compared with the low dose group:Motilin of high dose group was significantly higher than the low dose group（P<0.01),themiddle dose group, the domperidone group and the low dose groupMotilin was no significant difference（P>0.05）.④Compared with themiddle dose group:the high dose group, the domperidone group and themiddle dose group Motilin was no significant difference（P>0.05）.⑤Compared with the high dose group:Motilin of the group domperidonewas significantly lower than the high dose group （P<0.01).8.Comparision of Gastrin of rats in each group:①Compared with the blankcontrol group: Gastrin of model group, low dose group, middle dosegroup and domperidone group was significantly lower than the blankcontrol group（P<0.01),the high dose group and the blank control groupMotilin was no significant difference（P>0.05）.②Compared with themodel control group: Gastrin of low dose group, middle dose group, highdose group and the group domperidone was significantly higher than themodel control group （P<0.01).③Compared with the low dosegroup:Gastrin of high dose group was higher than the low dose group（P<0.05),the middle dose group, the domperidone group and the lowdose group Gastrin was no significant difference（P>0.05）.④Comparedwith the middle dose group:Gastrin of high dose group was higher thanthe middle dose group（P<0.05),the domperidone group and the middledose group Gastrin was no significant difference（P>0.05）.⑤Comparedwith the high dose group:Gastrin of the group domperidone was significantly lower than the high dose group（P<0.01).9.Comparision ofthe gray value of the ICC-positive cells in antral organization of rats ineach group:①Compared with the blank control group: the gray value ofthe ICC-positive cells in antral organization of model group, low dosegroup, middle dose group,high dose group and domperidone group wassignificantly lower than the blank control group（P<0.01).②Comparedwith the model control group: the gray value of the ICC-positive cells inantral organization of low dose group, middle dose group, high dosegroup and the group domperidone was significantly higher than the modelcontrol group（P<0.01).③Compared with the low dose group:the grayvalue of the ICC-positive cells in antral organization of high dose groupwas higher than the low dose group（P<0.05),the middle dose group, thedomperidone group and the low dose group of the gray value of theICC-positive cells in antral organization was no significant difference（P>0.05）.④Compared with the middle dose group:the gray value ofthe ICC-positive cells in antral organization of high dose group washigher than the middle dose group（P<0.05).the domperidone group andthe middle dose group of the gray value of the ICC-positive cells in antralorganization was no significant difference（P>0.05）.⑤Compared withthe high dose group:the gray value of the ICC-positive cells in antralorganization of the group domperidone was significantly lower than thehigh dose group（P<0.01).Conclusion:1." Tail clamp stimulation "and " irregular feeding " joint model is just only " tail clamp stimulation "or"irregular feeding " of FD rat model of improvement, able to replicate theideal FD rat model.2. The gray value of the ICC-positive cells in antralorganization of model group, low dose group, middle dose group anddomperidone group was lower than the high dose group，it’s means thehigh dose group, it’s means high dose group to increase the number ofthe gray value of the ICC-positive cells in antral organization comparedwith the mode control group, the low dose group, middle dose group anddomperidone group is better.3.High dose group of the Chaishao gastritisparticles of functional dyspepsia rat model’s motilin, gastrin and gastricemptying rate compared to the model control group, low dose group,middle dose group, domperidone group is better, it shows that the highdose group can be better to promote gastrointestinal motility.4.Thepossible mechanism of the treatment of Chaishao gastritis particles ofFD rats is，it influences the FD rat gastric antrum’s ICC, increasesthe number of antral the number of ICC, so improve in motilin andgastrin secretion, enhanced gastrointestinal motility, thereby promotinggastric emptying.

          柴芍胃炎顆粒對功能性消化不良模型大鼠胃竇Cajal間質(zhì)細胞表達的影響

摘要4-9Abstract9-16前言17-19材料與方法19-25結果25-36討論36-43結論43-44參考文獻44-47英文縮略詞表47-48致謝48-49綜述49-61    參考文獻55-61

  本文關鍵詞：柴芍胃炎顆粒對功能性消化不良模型大鼠胃竇Cajal間質(zhì)細胞表達的影響，由筆耕文化傳播整理發(fā)布。