本文關鍵詞：MicroRNA-21抑制淫羊藿素誘導肝星狀細胞凋亡的研究，由筆耕文化傳播整理發(fā)布。

        目的探討microRNA-21在淫羊藿素誘導人肝星狀LX-2細胞凋亡中的作用和機理。方法1、DAPI染色后熒光顯微鏡及Annexin V/PI雙染色后行流式細胞術檢測淫羊藿素刺激LX-2細胞后的凋亡。2、定量PCR檢測microRNA-21的表達。3、瞬時細胞轉染pre-microRNA-21和anti-microRNA-21調節(jié)microRNA-21的表達。4、Western blot觀察凋亡相關蛋白Bcl-2、Bax的表達。結果1、淫羊藿素劑量依賴性誘導LX-2細胞的凋亡(P<0.05)2、淫羊藿素劑量依賴性減低LX-2細胞microRNA-21的表達(P<0.05)3、上調microRNA-21的表達減少淫羊藿素誘導的LX-2細胞的凋亡(P<0.05),而下調microRNA-21的表達則增加細胞凋亡(P<0.05)4、淫羊藿素下調LX-2細胞Bcl-2蛋白的表達,上調Bax蛋白的表達。而microRNA-21部分阻斷了這種作用。結論MicroRNA-21介導了淫羊藿素誘導的肝星狀細胞凋亡。

    ObjectiveTo investigate the role of microRNA-21in icaritin-induced apoptosis of hepatic stellate LX-2cell and possible mechanism involved.Methods1.Detection of the apoptotic cells by4’,6-diamidino-2-phenylindole and flow cytometry.2.Real-time polymerase chain reaction was used to detect microRNA-21expression changes.3.Transient transfection with pre-microRNA-21and anti-microRNA-21to regulate microRNA-21expression.4. Western blot was used to examine the protein expression of Bcl-2and Bax.Results1.Icaritin dose-dependently increased the apoptotic of LX-2cells (P<0.05)2.Icaritin dose-dependently decreased the expression of microRNA-21in LX-2cells (P<0.05)3.Overexpression of microRNA-21reduced icaritin-induced LX-2cells apoptosis (P<0.05),but inhibition of microRNA-21expression increased icaritin-induced LX-2cells apoptosis(P<0.05).4.Icaritin downregulated the protein expression of Bcl-2and upregulated the protein expression of Bax in LX-2cells.MicroRNA-21partly reversed the protein expression of Bcl-2and Bax induced by icaritin.ConclusionsMicroRNA-21mediates icaritin-induced LX-2cells apoptosis.

MicroRNA-21抑制淫羊藿素誘導肝星狀細胞凋亡的研究

英文縮略詞表5-7中文摘要7-8Abstract8引言9-15前言15材料和方法15-25結果25-32討論32-34小結34-35結論35-36參考文獻36-43綜述43-53    參考文獻50-53致謝53-54

  本文關鍵詞：MicroRNA-21抑制淫羊藿素誘導肝星狀細胞凋亡的研究，由筆耕文化傳播整理發(fā)布。