本文關(guān)鍵詞：MicroRNA-21抑制淫羊藿素誘導(dǎo)肝星狀細(xì)胞凋亡的研究，由筆耕文化傳播整理發(fā)布。

        目的探討microRNA-21在淫羊藿素誘導(dǎo)人肝星狀LX-2細(xì)胞凋亡中的作用和機理。方法1、DAPI染色后熒光顯微鏡及Annexin V/PI雙染色后行流式細(xì)胞術(shù)檢測淫羊藿素刺激LX-2細(xì)胞后的凋亡。2、定量PCR檢測microRNA-21的表達(dá)。3、瞬時細(xì)胞轉(zhuǎn)染pre-microRNA-21和anti-microRNA-21調(diào)節(jié)microRNA-21的表達(dá)。4、Western blot觀察凋亡相關(guān)蛋白Bcl-2、Bax的表達(dá)。結(jié)果1、淫羊藿素劑量依賴性誘導(dǎo)LX-2細(xì)胞的凋亡(P<0.05)2、淫羊藿素劑量依賴性減低LX-2細(xì)胞microRNA-21的表達(dá)(P<0.05)3、上調(diào)microRNA-21的表達(dá)減少淫羊藿素誘導(dǎo)的LX-2細(xì)胞的凋亡(P<0.05),而下調(diào)microRNA-21的表達(dá)則增加細(xì)胞凋亡(P<0.05)4、淫羊藿素下調(diào)LX-2細(xì)胞Bcl-2蛋白的表達(dá),上調(diào)Bax蛋白的表達(dá)。而microRNA-21部分阻斷了這種作用。結(jié)論MicroRNA-21介導(dǎo)了淫羊藿素誘導(dǎo)的肝星狀細(xì)胞凋亡。

    ObjectiveTo investigate the role of microRNA-21in icaritin-induced apoptosis of hepatic stellate LX-2cell and possible mechanism involved.Methods1.Detection of the apoptotic cells by4’,6-diamidino-2-phenylindole and flow cytometry.2.Real-time polymerase chain reaction was used to detect microRNA-21expression changes.3.Transient transfection with pre-microRNA-21and anti-microRNA-21to regulate microRNA-21expression.4. Western blot was used to examine the protein expression of Bcl-2and Bax.Results1.Icaritin dose-dependently increased the apoptotic of LX-2cells (P<0.05)2.Icaritin dose-dependently decreased the expression of microRNA-21in LX-2cells (P<0.05)3.Overexpression of microRNA-21reduced icaritin-induced LX-2cells apoptosis (P<0.05),but inhibition of microRNA-21expression increased icaritin-induced LX-2cells apoptosis(P<0.05).4.Icaritin downregulated the protein expression of Bcl-2and upregulated the protein expression of Bax in LX-2cells.MicroRNA-21partly reversed the protein expression of Bcl-2and Bax induced by icaritin.ConclusionsMicroRNA-21mediates icaritin-induced LX-2cells apoptosis.

MicroRNA-21抑制淫羊藿素誘導(dǎo)肝星狀細(xì)胞凋亡的研究

英文縮略詞表5-7中文摘要7-8Abstract8引言9-15前言15材料和方法15-25結(jié)果25-32討論32-34小結(jié)34-35結(jié)論35-36參考文獻(xiàn)36-43綜述43-53    參考文獻(xiàn)50-53致謝53-54

  本文關(guān)鍵詞：MicroRNA-21抑制淫羊藿素誘導(dǎo)肝星狀細(xì)胞凋亡的研究，由筆耕文化傳播整理發(fā)布。