本文選題：桃仁提取物 + 急性胰腺炎�。� 參考：《中國(guó)免疫學(xué)雜志》2015年03期

【摘要】：目的:研究不同劑量桃仁提取物對(duì)急性胰腺炎大鼠腸道黏膜屏障功能及免疫功能的作用。方法:48只大鼠制備SAP模型后隨機(jī)分為模型對(duì)照組、桃仁提取物低劑量、中劑量和高劑量組,每組12只。另取12只大鼠作為假手術(shù)組,造模麻醉蘇醒即開始干預(yù),桃仁提取物低劑量組、中劑量組和高劑量組分別灌胃給予桃仁提取物0.12、0.248和0.36 g/kg,假手術(shù)組及模型組給予等體積蒸餾水灌胃,各組灌胃均1次/6 h,連續(xù)4次。給藥后24 h應(yīng)用10%水合氯醛麻醉各組大鼠,打開胸腔和腹腔,腹主動(dòng)脈分別抽取5 ml血樣于EDTA抗凝管和非抗凝管內(nèi),分別應(yīng)用熒光直接標(biāo)記法和流式細(xì)胞儀進(jìn)行CD4+、CD8+和Treg細(xì)胞測(cè)定,采用免疫比濁法測(cè)定Ig A、Ig G和Ig M,采用EPS-G7底物法測(cè)定血清淀粉酶水平,采用酶學(xué)分光光度法檢測(cè)血清D-乳酸水平,采用活性比色法測(cè)定血清二胺氧化酶;取小腸組織HE染色后采用光學(xué)顯微鏡進(jìn)行病理學(xué)檢查;取小腸組織采用放射免疫法進(jìn)行s Ig A測(cè)定以及采用RT-PCR法進(jìn)行TLR4和NF-κBp65 mRNA測(cè)定。結(jié)果:(1)中劑量和高劑量組大鼠血清淀粉酶、D-乳酸和二胺氧化酶水平均較低劑量組大鼠顯著降低(P0.01),小腸黏膜s Ig A較低劑量組顯著升高(P0.01),并且高劑量組和中劑量組差異具有統(tǒng)計(jì)學(xué)意義(P0.01);(2)桃仁提取物中劑量和高劑量組血液CD4+、CD4+/CD8+較低劑量組大鼠顯著升高(P0.01),CD8+、Treg細(xì)胞較低劑量組大鼠顯著降低(P0.01),并且高劑量組和中劑量組差異均具有統(tǒng)計(jì)學(xué)意義(P0.01);(3)桃仁提取物中劑量和高劑量組血清Ig A、Ig G、Ig M較低劑量組大鼠顯著升高(P0.01),并且高劑量組和中劑量組差異均具有統(tǒng)計(jì)學(xué)意義(P0.01);(4)假手術(shù)組大鼠小腸黏膜無(wú)顯著損傷,模型對(duì)照組大鼠小腸黏膜顯著損傷,低劑量組大鼠小腸黏膜病理情況與模型組基本相似,中劑量和大劑量組大鼠小腸黏膜損傷顯著降低;(5)桃仁提取物中劑量和高劑量組小腸組織TLR4和NF-κBp65 mRNA較低劑量組大鼠顯著降低(P0.01),并且高劑量組和中劑量組差異均具有統(tǒng)計(jì)學(xué)意義(P0.01)。結(jié)論:桃仁提取物對(duì)急性胰腺炎大鼠腸道屏障功能具有保護(hù)作用,并且顯著改善急性胰腺炎大鼠的免疫功能。
[Abstract]:Aim: to study the effects of different doses of peach kernel extract on intestinal mucosal barrier function and immune function in rats with acute pancreatitis.Methods SAP model was established in 48 rats. The rats were randomly divided into control group, low dose group, middle dose group and high dose group with 12 rats in each group.Another 12 rats were taken as sham-operation group, the anaesthesia recovery of model group began to intervene, the low dose group of peach kernel extract,The middle dose group and the high dose group were given 0.12g / kg and 0.36 g / kg of peach kernel extract respectively. The sham operation group and the model group were given the same volume distilled water. The rats in each group were given intragastric perfusion for 1 / 6 h for 4 consecutive times.24 hours after administration, 10% chloral hydrate was used to anesthetize the rats in each group. The thoracic cavity and abdominal cavity were opened, and 5 ml blood samples were taken from abdominal aorta in EDTA anticoagulant tube and non-anticoagulant tube respectively.CD4 CD8 and Treg cells were detected by fluorescence direct labeling method and flow cytometry respectively. The levels of serum amylase were measured by immunoturbidimetric assay (Ig G) and IgM (IgM). The serum amylase levels were measured by EPS-G7 substrate method.The level of serum D-lactic acid was detected by enzymatic spectrophotometry, the serum diamine oxidase was determined by active colorimetry, the tissues of small intestine were stained by HE and the pathology was examined by optical microscope.Small intestinal tissues were detected by radioimmunoassay (RIA) and TLR4 and NF- 魏 Bp65 mRNA (NF- 魏 Bp65 mRNA) by RT-PCR method.Results the levels of serum amylase D-lactic acid and diamine oxidase in the middle and high dose groups were significantly lower than those in the low dose group, and the levels of SIG A in small intestine mucosa were significantly higher than those in the low dose group, and the levels of high and medium dose groups were significantly higher than those in the low dose group.The difference was statistically significant (P 0.01)) the blood CD4 CD 4 / CD 8 in the middle dose group and the high dose group were significantly higher than those in the low dose group. The number of P0.01T 8 / CD 8 Treg cells in the low dose group was significantly lower than that in the low dose group, and there was a significant difference between the high dose group and the middle dose group, and the difference between the high dose group and the middle dose group was also found in the difference between the high dose group and the middle dose group.Compared with the low dose group, the serum Ig G G G M increased significantly, and the difference between the high dose group and the middle dose group was statistically significant (P 0.01). There was no significant damage to the intestinal mucosa in the sham operation group, and the difference between the high dose group and the middle dose group was statistically significant (P 0.01), and the difference between the high dose group and the middle dose group was statistically significant (P 0.01), and there was no significant damage to the intestinal mucosa in the sham operation group.The pathological changes of intestinal mucosa in the low dose group were similar to those in the model group.The TLR4 and NF- 魏 Bp65 mRNA of small intestine tissue in the middle and high dose groups were significantly lower than those in the low dose group, and the difference between the high dose group and the middle dose group was statistically significant (P 0.01).Conclusion: Peach kernel extract has protective effect on intestinal barrier function in rats with acute pancreatitis and improves the immune function of acute pancreatitis rats.
【作者單位】： 河北省滄州市人民醫(yī)院普外二科;
【基金】：河北省中醫(yī)藥管理局科研計(jì)劃資助項(xiàng)目《桃仁提取物在急性胰腺炎致腸道微循環(huán)障礙中的作用及機(jī)制研究》(No.2013141)
【分類號(hào)】：R576

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