本文選題：白族　切入點：腸道菌群　出處：《微生物學(xué)通報》2015年03期

【摘要】：【目的】通過研究云南白族成年人腸道菌群群落結(jié)構(gòu),探索腸道菌群結(jié)構(gòu)與云南白族人健康長壽的相互關(guān)系�！痉椒ā恳�43份采自云南省昆明市(Urban)和大理白族自治州洱源縣農(nóng)村(Rural)成年白族志愿者的糞便樣品為研究對象,通過種屬特異性聚合酶鏈?zhǔn)椒磻?yīng)(定性PCR)和基于聚合酶鏈?zhǔn)椒磻?yīng)的變性梯度凝膠電泳(PCR-DGGE)對其中的優(yōu)勢菌群進(jìn)行了分析,并測定樣品中短鏈脂肪酸(SCFAs)含量�！窘Y(jié)果】短鏈脂肪酸(SCFAs)含量測定結(jié)果顯示,Rural和Urban志愿者的短鏈脂肪酸含量差異不顯著;定性PCR結(jié)果顯示Rural志愿者腸道內(nèi)的乳桿菌屬和雙歧桿菌屬的多樣性明顯區(qū)別于Urban志愿者;依據(jù)泳道軌跡光密度的不同對16S r RNA基因V3區(qū)PCR-DGGE圖譜進(jìn)行基于非加權(quán)平均距離聚類分析(Unweighted pair-group method with arithmetic means,UPGMA),顯示兩組樣品按照組別明顯地聚為兩簇,多樣性指數(shù)分析也呈現(xiàn)Rural志愿者腸道菌群多樣性大于Urban的趨勢;進(jìn)一步對Rural譜帶回收克隆測序,并構(gòu)建系統(tǒng)發(fā)育樹圖,結(jié)果顯示樣品中雙歧桿菌屬、腸桿菌屬和腸球菌屬具有較高的多樣性,是Rural健康成年志愿者腸道中的優(yōu)勢菌群�！窘Y(jié)論】綜合上述分析,昆明城市志愿者和大理白族自治州洱源縣農(nóng)村志愿者腸道菌群群落結(jié)構(gòu)呈現(xiàn)區(qū)分趨勢,多樣性差異不顯著。該研究為后續(xù)探索宿主腸道菌群與健康長壽的相互關(guān)系提供了數(shù)據(jù)基礎(chǔ)。
[Abstract]:[objective] to study the intestinal microflora community structure of Bai nationality adults in Yunnan Province.To explore the relationship between intestinal flora structure and the health and longevity of Bai people in Yunnan Province. [methods] the feces samples of 43 adult Bai volunteers from Urbane, Kunming, Yunnan Province, and Ruralin County, Eryuan County, Dali Bai Autonomous Prefecture, were studied.Species specific polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) based on polymerase chain reaction (PCR) were used to analyze the dominant microflora.The short chain fatty acid (SCFASS) content in the samples was determined. [results] the results showed that there was no significant difference in SCFASs between Urban volunteers and rural volunteers.Qualitative PCR results showed that the diversity of Lactobacillus and Bifidobacterium in the intestinal tract of Rural volunteers was significantly different from that of Urban volunteers.Based on the unweighted pair-group method with arithmetic meansman, the PCR-DGGE map of the V3 region of the 16s r RNA gene was analyzed according to the different optical density of the swimming track. The results showed that the two groups of samples were obviously clustered into two clusters according to the group.The analysis of diversity index also showed that the diversity of intestinal flora of Rural volunteers was greater than that of Urban, and the Rural bands were sequenced and phylogenetic tree map was constructed. The results showed that Bifidobacterium in the sample was bifidobacterium.Enterobacter and Enterococcus have a high diversity and are the dominant flora in the intestinal tract of healthy adult Rural volunteers. [conclusion] the above analysis is comprehensive.The community structure of intestinal flora of volunteers from Kunming city and Eryuan County of Dali Bai Autonomous Prefecture showed a distinguishing trend, but the diversity was not significant.This study provides a data basis for further exploring the relationship between intestinal flora and health and longevity.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)乳品生物技術(shù)與工程教育部重點實驗室;
【基金】：國家自然科學(xué)基金項目(No.30660135)
【分類號】：R573

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