本文選題：腸道菌群失調　切入點：內(nèi)臟高敏感　出處：《西安交通大學學報(醫(yī)學版)》2015年06期

【摘要】：目的檢測腸道菌群失調大鼠的內(nèi)臟敏感性變化及緊密連接蛋白(ZO-1)、Toll樣4受體(TLR4)的表達,探討腸道菌群失調對內(nèi)臟敏感性的影響及其機制。方法 SPF級SD大鼠30只,隨機分為正常對照組(12只)、菌群失調組(18只)。菌群失調組采用鹽酸林可霉素(300mg/mL)灌胃,1mL/(次·只),1次/d,連續(xù)給藥7d;正常對照組采用等量生理鹽水灌胃。第8天隨機抽取菌群失調組和正常對照組大鼠各6只,檢測模型是否成功。模型建立成功后,將其余12只菌群失調組大鼠隨機分組:陰性對照組、益生菌干預組,每組6只。益生菌干預組給予雙歧桿菌三聯(lián)活菌膠囊(培菲康)灌胃,1粒/3mL生理鹽水,1mL/(次·只),1次/d,連續(xù)給藥7d;陰性對照組給予同等量生理鹽水灌胃。第8天進行糞便菌群培養(yǎng)、檢測內(nèi)臟敏感性、檢測結腸組織中ZO-1及TLR4的mRNA和蛋白表達、檢測血清中炎性因子IL-10、TNFα的表達。結果與正常對照組比較,菌群失調組大鼠結腸ZO-1的mRNA含量及蛋白表達顯著降低,而結腸TLR4的含量及蛋白表達顯著升高,且血清中促炎因子TNFα表達顯著升高,而抑炎因子IL-10的表達顯著降低(P0.05)。與菌群失調組相比,益生菌干預組結腸ZO-1的mRNA含量及蛋白表達顯著升高,而結腸TLR4的mRNA含量及蛋白表達顯著降低,且血清中促炎因子TNFα表達顯著降低而抑炎因子IL-10的表達顯著升高(P0.05)。結論通過抑制結腸中ZO-1的表達及升高TLR4的表達,進而導致慢性低度炎癥的發(fā)生可能是腸道菌群失調導致內(nèi)臟高敏感的作用機制之一,同時益生菌制劑可能通過恢復腸道菌群變化進而改善內(nèi)臟高敏感。
[Abstract]:Objective to detect the changes of visceral sensitivity and the expression of TLR4, a TLR4 receptor, in rats with intestinal dysbacteriosis, and to explore the effect of intestinal dysbacteriosis on visceral sensitivity and its mechanism.Methods Thirty SPF SD rats were randomly divided into normal control group (n = 12) and dysbacteriosis group (n = 18).The patients in the dysbacteriosis group were treated with lincomycin hydrochloride (300mg / mL) and the control group were given the same amount of normal saline for 7 days.On the 8th day, 6 rats in the dysbacteriosis group and 6 rats in the normal control group were randomly selected to detect the success of the model.After the establishment of the model, the remaining 12 rats in the dysbacteriosis group were randomly divided into negative control group and probiotic intervention group with 6 rats in each group.The probiotics intervention group was given bifidobacterium triple live bacilli capsule (Peifeikang) 1 / 3 mL normal saline 1 mL / L / 1 (once a day for 7 consecutive days), while the negative control group was given the same amount of normal saline intragastrically.The fecal flora was cultured on the 8th day to detect the visceral sensitivity, the expression of mRNA and protein of ZO-1 and TLR4 in colonic tissue, and the expression of IL-10 TNF- 偽 in serum.Results compared with the normal control group, the expression of ZO-1 mRNA and protein in colonic tissue of rats with dysbacteriosis decreased significantly, while the expression of TLR4 and protein in colon increased significantly, and the expression of proinflammatory factor TNF 偽 increased significantly in serum of rats with dysbacteriosis.However, the expression of anti-inflammatory factor IL-10 was significantly lower than that of P0.05.Compared with the dysbacteriosis group, the mRNA content and protein expression of colon ZO-1 in probiotic intervention group increased significantly, while the mRNA content and protein expression of colon TLR4 decreased significantly in probiotic intervention group.Moreover, the expression of proinflammatory factor TNF 偽 in serum decreased significantly, while the expression of anti inflammatory factor IL-10 increased significantly (P 0.05).Conclusion by inhibiting the expression of ZO-1 and increasing the expression of TLR4 in colon, the occurrence of chronic low-grade inflammation may be one of the mechanisms of intestinal flora imbalance and visceral hypersensitivity.At the same time, probiotics may improve visceral hypersensitivity by restoring intestinal flora changes.
【作者單位】： 西安交通大學醫(yī)學部第二附屬醫(yī)院消化內(nèi)科陜西省腸胃動力疾病研究重點實驗室陜西省胃腸疾病臨床研究中心;西安交通大學醫(yī)學部第二附屬醫(yī)院核醫(yī)學科;
【分類號】：R574.4

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