本文選題：IFN-γ　切入點：基因多態(tài)性　出處：《青海大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的：通過青海地區(qū)藏族人群中IFN-γ基因多態(tài)性的2個位點與慢性乙肝易感性之間的關系，篩選出主要相關位點，為預測青海地區(qū)藏族人群的HBV易感性的風險奠定基礎。進而為乙肝的臨床診斷及預防提供新的理論和依據(jù)。方法：（1）研究對象：擬選自2012年6月-2013年9月期間在青海省傳染病醫(yī)院和青海大學附屬醫(yī)院經(jīng)臨床及實驗室檢查確診的青海地區(qū)慢性乙肝藏族患者250例（病例組）及健康志愿者134例(對照組)為研究對象。（2）基因多態(tài)性分析：采用試劑盒提取外周血基因組DNA，采用飛行質(zhì)譜技術分析檢測基因γ-干擾素的+874A/A、+874A/T、+874T/T、+2109C/C、+2109C/T、+2109T/T的基因型。（3）數(shù)據(jù)分析：計算出各位點基因型及等位基因頻率，，分析組間各基因型的差異性，如若P＜0.05，還需應用logistic多元回歸，進一步分析各基因型與慢性乙肝易感性之間的關系,若P＞0.05，則基因位點的多態(tài)性與乙肝易感性則無關。結果：（1）+2109位點在病例組以及對照組之間的頻率分布：①+2109C/C在病例組和對照組分別為10.5%和14.3%，經(jīng)統(tǒng)計學分析結果表明,各組間的分布頻率未見顯著性差異；②+2109C/T在病例組和對照組分別為47.3%和43.7%，經(jīng)統(tǒng)計學分析結果表明,各組間的分布頻率未見顯著性差異；③+2109T/T在病例組和對照組分別為42.2%和42.1%，經(jīng)統(tǒng)計學分析結果表明,各組間的分布頻率未見顯著性差異；（2）+874位點在病例組以及對照組之間的頻率分布:①+874A/A在病例組和對照組分別為3.1%和2.4%，經(jīng)統(tǒng)計學分析結果表明,各組間的分布頻率未見顯著性差異；②+874A/T在病例組和對照組分別為30.6%和19.8%，經(jīng)統(tǒng)計學分析結果表明,各組間的分布頻率未見顯著性差異；③+874T/T在病例組和對照組分別為66.3%和77.8%，經(jīng)統(tǒng)計學分析結果表明,各組間的分布頻率未見顯著性差異；（3）+874等位基因T在病例組和對照組基因頻率分別為81.6%和87.7%，經(jīng)統(tǒng)計學分析攜帶等位基因T的頻率升高表明乙肝的易感性越低。結論：(1)IFN-γ+874基因位點多態(tài)性與青海地區(qū)藏族人群HBV感染的易感性可能無關；(2)IFN-γ+2109基因位點多態(tài)性與青海地區(qū)藏族人群HBV感染的易感性可能無關；(3)IFN-γ+874的等位基因T可能是青海藏族乙肝的保護性基因。
[Abstract]:Objective: to study the relationship between the two loci of IFN- 緯 gene polymorphism and the susceptibility to chronic hepatitis B in Tibetan population in Qinghai area, and to screen out the main related sites. In order to predict the risk of HBV susceptibility of Tibetan population in Qinghai area, and provide a new theory and basis for clinical diagnosis and prevention of hepatitis B. methods: selected from June 2012 to September 2013 in Qinghai Province. Genetic Polymorphism of 250 Tibetan patients with chronic Hepatitis B (case Group) and 134 healthy volunteers (Control Group) confirmed by Clinical and Laboratory examination in Hospital of Infectious Diseases and affiliated Hospital of Qinghai University. Sex analysis: genomic DNA was extracted from peripheral blood by kit, and 874 A / A, 874 A / T, 874 T / T, 2109 C / T, 2109 C / T, 2109T/T genotypes of interferon 緯 were analyzed by flight mass spectrometry (FMS). If P < 0.05, logistic multiple regression should be used to further analyze the relationship between genotype and chronic hepatitis B susceptibility. If P > 0.05, the polymorphism of the gene locus was not related to the susceptibility to hepatitis B. results the frequency distribution of 2109 2109C/C in the case group and the control group was 10.5% and 14.33%, respectively. There was no significant difference in the frequency of distribution between the two groups (47.3% and 43.7% in the case group and the control group, respectively). The results of statistical analysis showed that there was no significant difference between the two groups. There was no significant difference in the distribution frequency among the three groups (42.2% and 42.1% in the case group and the control group, respectively). There was no significant difference in the distribution frequency between each group and control group. The frequency distribution of 1: 1 874A/A in case group and control group was 3.1% and 2.4%, respectively. The results of statistical analysis showed that there was no significant difference between the two groups. There was no significant difference in the frequency of distribution between each group (30.6%) and control group (19.8%). The results of statistical analysis showed that the frequency of the two groups was 30.6% and 19.8%, respectively. There was no significant difference in the distribution frequency among the three groups (66.3% and 77.8% in the case group and the control group, respectively). There was no significant difference in the distribution of allele T among the three groups. The frequency of allele T was 81.6% in the case group and 87.7% in the control group. The higher the frequency of allele T was, the lower the susceptibility of hepatitis B was. It is suggested that the polymorphism of IFN- 緯 874 gene locus is not related to the susceptibility to HBV infection in Tibetan population in Qinghai area. The polymorphism of IFN- 緯 2109 gene locus may not be related to the susceptibility to HBV infection of Tibetan population in Qinghai area, and the allele T of IFN- 緯 874 may be as follows:. The protective gene of hepatitis B in Qinghai Tibetan nationality.
【學位授予單位】：青海大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R512.62

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