發(fā)布時(shí)間：2018-03-17 20:44

  本文選題：肝纖維化　切入點(diǎn)：Smurf　出處：《廣西醫(yī)科大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:觀察Smurf1、Smurf2、Smad3和Smad7蛋白在人肝纖維化組織中的表達(dá)，探討四者的相互關(guān)系及介導(dǎo)的信號(hào)傳導(dǎo)在肝纖維化發(fā)生中的作用機(jī)制。 方法:采用免疫組織化學(xué)法測(cè)定9例正常肝組織和38例慢性HBV感染者肝組織中Smurf1、Smurf2、Smad3和Smad7的表達(dá)情況。 結(jié)果:Smurf1、Smurf2、Smad3和Smad7在肝內(nèi)實(shí)質(zhì)細(xì)胞及非實(shí)質(zhì)細(xì)胞均可見廣泛表達(dá)。與正常肝相比，肝纖維化組Smad3表達(dá)顯著增加（Z=-3.110，P=0.002），Smurf2表達(dá)亦明顯增加（Z=-2.716，P=0.007），Smad7表達(dá)顯著降低（Z=-2.899，P=0.004）；而Smurf1表達(dá)無顯著變化（Z=-1.399，P=0.162），差異無統(tǒng)計(jì)學(xué)意義。Smad3與纖維化程度呈顯著正相關(guān)（r=0.627，P=0.000）；Smad7與纖維化程度呈顯著負(fù)相關(guān)（r=-0.488，P=0.000）；Smurf1與肝纖維化無顯著相關(guān)性（r=-0.064，P=0.670），Smurf2與纖維化程度呈顯著正相關(guān)（r=0.652，P=0.000）。Smurf2與Smad3呈顯著正相關(guān)（r=0.523，，P=0.000），與Smad7呈顯著負(fù)相關(guān)（r=-0.447，P=0.002）；Smurf1與Smurf2、Smad3、Smad7無相關(guān)性（r=-0.008、r=-0.053、r=0.219，P＞0.05）；Smad3與Smad7呈負(fù)直線相關(guān)（r=-0.389，P=0.007）。 結(jié)論：Smad3信號(hào)增強(qiáng)及Smad7信號(hào)缺失可能導(dǎo)致肝纖維化發(fā)展,Smurf1在肝纖維化的發(fā)展過程中能發(fā)揮的調(diào)控作用有限，Smurf2在肝纖維化進(jìn)展中發(fā)揮雙向調(diào)節(jié)作用,但對(duì)TGF-β1/Smad信號(hào)通路的促進(jìn)作用大于其抑制作用。
[Abstract]:Aim: to investigate the expression of Smurf1, Smurf2, Smad3 and Smad7 proteins in human liver fibrosis, and to explore the relationship between the four proteins and the mechanism of signal transduction in the pathogenesis of liver fibrosis. Methods: the expression of Smurf1 Smurf2 Smad3 and Smad7 in 9 cases of normal liver tissue and 38 cases of chronic HBV infection were detected by immunohistochemical method. Results both Smurf2 Smad3 and Smad7 were widely expressed in parenchymal cells and non-parenchymal cells. In liver fibrosis group, the expression of Smad3 increased significantly, and the expression of Smurf2 increased significantly in the liver fibrosis group. The expression of Smad7 decreased significantly, while the expression of Smurf1 did not change significantly. There was no significant difference between Smad3 and the degree of fibrosis. There was a significant negative correlation between Smad7 and the degree of fibrosis. There was no significant correlation between Smurf1 and liver fibrosis. There was a significant positive correlation between Smurf2 and the degree of fibrosis. There was a significant positive correlation between Smurf2 and Smad3. There was a significant negative correlation between Smurf1 and Smurf1 and Smurf1, Smurf1 and Smurf2Smad3Smad7. There was no significant correlation between Smurf1 and Smurf2 and Smurf2Smad3Smad7. There was no significant correlation between Smurf1 and Smurf2 and Smurf2Smad3Smad7. There was no significant positive correlation between Smurf1 and Smurf2 and the degree of fibrosis. There was a significant positive correlation between Smurf2 and Smad3. There was no significant correlation between Smurf1 and Smurf1 and Smurf2Smad3Smad7. There was no significant positive correlation between Smurf1 and Smurf2 and Smurf2 were positively correlated with Smad3, and there was no significant correlation between Smurf1 and Smurf1 with Smurf2Smad3Smad7. Conclusion the enhancement of Smad3 signal and the absence of Smad7 signal may lead to the development of hepatic fibrosis. Smurf1 may play a limited regulatory role in the development of liver fibrosis. Smurf2 may play a bidirectional regulatory role in the progression of liver fibrosis. However, the promotion of TGF- 尾 1 / Smad signaling pathway was greater than its inhibitory effect.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R575

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 張國,張法燦,王天才,梁擴(kuò)寰;活血軟堅(jiān)方對(duì)肝星狀細(xì)胞Smad信號(hào)的影響及意義[J];中華肝臟病雜志;2004年04期

2 陳偉 ,付小兵 ,盛志勇;Review of current progress in the structure and function of Smad proteins[J];Chinese Medical Journal;2002年03期

本文編號(hào)：1626402