本文關(guān)鍵詞： 丙型肝炎病毒 ELISA FQ-PCR ALT AST　出處：《廣東醫(yī)學(xué)》2015年24期 　論文類型：期刊論文

【摘要】：目的探討酶聯(lián)免疫吸附試驗(yàn)(ELISA)法檢測抗-HCV-IgG抗體以及實(shí)時(shí)熒光定量聚合酶鏈反應(yīng)(FQ-PCR)檢測血清HCV-RNA在丙型肝炎診斷中的應(yīng)用價(jià)值。方法對70例肝病科及腎病科丙型肝炎病毒(HCV)待查者血清標(biāo)本,同時(shí)采用ELISA法檢測抗-HCV-IgG抗體滴度,FQ-PCR法檢測HCV-RNA載量,全自動生化分析儀檢測ALT、AST,分析不同檢測結(jié)果組合類型的HCV-RNA載量或抗-HCV-IgG抗體滴度與ALT、AST、AST/ALT值的關(guān)系。結(jié)果在70例血清標(biāo)本中,用ELISA法檢測到抗-HCV-IgG(+)(S/C.O.值≥1)有49例,使用FQ-PCR法檢測到HCV-RNA(+)(載量≥5.0×102IU/m L)有51例。男女之間感染率差異無統(tǒng)計(jì)學(xué)意義(P0.05),但不同年齡組感染率差異有統(tǒng)計(jì)學(xué)意義(P0.05)。單陽性型患者與雙陽性型患者的血清ALT異常率、AST異常率或AST/ALT倒置率(AST/ALT1)差異均無統(tǒng)計(jì)學(xué)意義(均P0.05)。HCV-RNA(+)且抗-HCV-IgG(+)患者的lgHCV-RNA載量與抗-HCV-IgG抗體S/C.O.值、ALT水平均呈線性正相關(guān)(r值分別為0.586、0.521,均P0.05),但是與AST/ALT值不呈線性相關(guān)(r=-0.076,P0.05)。HCV-RNA(+)且抗-HCV-IgG(-)患者的lg HCV-RNA載量與ALT水平、AST/ALT值均無直線相關(guān)關(guān)系存在(r值分別為-0.333、0.611,均P0.05)。HCV-RNA(-)且抗-HCV-Ig G(+)患者的抗-HCV-IgG抗體S/C.O.值與ALT水平、AST/ALT值也無直線相關(guān)關(guān)系存在(r值分別為0.485、0.563,均P0.05)。結(jié)論單一使用ELISA法檢測抗-HCV-IgG抗體或FQ-PCR法檢測HCV-RNA,對于丙型肝炎的診斷都有一定局限性。同時(shí)應(yīng)用ELISA法和FQ-PCR法進(jìn)行檢測時(shí),不同結(jié)果組合類型的患者,其HCV-RNA載量或抗-HCV-IgG抗體S/C.O.值與ALT水平、AST/ALT值的相關(guān)性并不一致。
[Abstract]:Objective to study the detection of anti-HCV-IgG antibody and real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) by Elisa. Methods the serum samples of 70 patients with hepatitis C virus in hepatology and nephrology were studied. At the same time, ELISA method was used to detect the titer of anti-HCV-IgG antibody and FQ-PCR method was used to detect HCV-RNA load, and automatic biochemical analyzer was used to detect alt. The relationship between the HCV-RNA load or anti-HCV-IgG antibody titer and the alt value of alt was analyzed. The results were obtained in 70 serum samples. There were 49 cases of anti-HCV-IgG (S / C. O. Value 鈮，

本文編號：1490378