發(fā)布時(shí)間：2018-01-08 03:29

  本文關(guān)鍵詞：七氟醚預(yù)處理對梗阻性黃疸肝缺血再灌注大鼠腎損傷的影響及機(jī)制　出處：《山東醫(yī)藥》2016年12期 　論文類型：期刊論文

  更多相關(guān)文章： 梗阻性黃疸 缺血再灌注 七氟醚 丙二醇 超氧化物歧化酶 三磷酸腺苷

【摘要】：目的觀察七氟醚對梗阻性黃疸肝缺血再灌注大鼠腎損傷的影響,探討其作用機(jī)制。方法將60只SD大鼠隨機(jī)分成三組,每組20只。模型組制備梗阻性黃疸肝缺血再灌注模型;七氟醚預(yù)處理組吸入七氟醚30 min、吸入空氣30 min,制備梗阻性黃疸肝缺血再灌注模型;正常對照組僅手術(shù)游離肝門至膽總管。分別于處理1、3、6、12、24 h每組隨機(jī)選取4只大鼠行下腔靜脈采血,檢測血清肌酐(Cr)、尿素氮(BUN)。處死大鼠,取左腎組織,光鏡下觀察腎組織病理學(xué)變化;制備腎組織勻漿,檢測腎組織丙二醛(MDA)、超氧化物歧化酶(SOD)、三磷酸腺苷(ATP)。結(jié)果對照組不同時(shí)間點(diǎn)腎小管及腎間質(zhì)結(jié)構(gòu)正常;模型組腎臟近曲小管上皮細(xì)胞濁腫,并出現(xiàn)細(xì)胞崩析、廣泛細(xì)胞管形成;七氟醚預(yù)處理組腎組織改變同模型組,但程度均減輕。與對照組比較,模型組及七氟醚預(yù)處理組各時(shí)間點(diǎn)血清Cr、BUN水平均升高(P均0.01);七氟醚預(yù)處理組各時(shí)間點(diǎn)血清Cr和BUN水平均低于模型組(P0.05或0.01)。與對照組比較,模型組及七氟醚預(yù)處理組各時(shí)間點(diǎn)腎組織MDA升高,SOD、ATP降低,組間比較P均0.01;七氟醚預(yù)處理組各時(shí)間點(diǎn)腎組織MDA低于模型組,SOD、ATP高于模型組,組間比較P0.05或0.01。結(jié)論七氟醚預(yù)處理可減輕梗阻性黃疸肝缺血再灌注大鼠腎損傷,其機(jī)制可能與減少氧自由基產(chǎn)生有關(guān)。
[Abstract]:Objective to observe the effect of sevoflurane on renal injury in rats with obstructive jaundice liver ischemia reperfusion. Methods 60 SD rats were randomly divided into three groups. 20 rats in each group. The model group was used to establish hepatic ischemia-reperfusion model of obstructive jaundice. Sevoflurane preconditioning group inhaled sevoflurane for 30 min and inhaled air for 30 min to establish hepatic ischemia-reperfusion model of obstructive jaundice. The normal control group only operated on free hepatic hilum to common bile duct. 4 rats in each group were randomly selected to collect blood from the inferior vena cava for 24 h after treatment of 1: 3, 6, 12 and 24 h, and the serum creatinine (Cr) was detected. The rats were killed and left renal tissue was taken. The pathological changes of renal tissue were observed under light microscope. The renal homogenate was prepared, and the renal tissue malondialdehyde (MDA), superoxide dismutase (SOD) and adenosine triphosphate (ATP) were detected. Results the renal tubule and interstitial structure of the control group were normal at different time points. In the model group, the epithelial cells of proximal convoluted tubules in the kidney were turbid, the cells collapsed and a wide range of cell tubes were formed. The changes of renal tissue in sevoflurane preconditioning group were similar to those in model group, but the degree was reduced. Compared with control group, the serum Cr bun levels in model group and sevoflurane pretreatment group were increased at each time point (P < 0.01). The serum Cr and BUN levels in sevoflurane pretreatment group were lower than those in model group (P0.05 or 0.01). In model group and sevoflurane preconditioning group, MDA in renal tissue increased and decreased at each time point, compared with each other (P 0.01). The MDA of renal tissue in sevoflurane pretreatment group was lower than that in model group at each time point. Conclusion Preconditioning with sevoflurane can reduce renal injury in rats with obstructive jaundice after hepatic ischemia reperfusion, and its mechanism may be related to reducing the production of oxygen free radicals.
【作者單位】： 中山大學(xué)附屬第五醫(yī)院;
【基金】：珠海市科技計(jì)劃項(xiàng)目(2014D0401990017)
【分類號】：R575
【正文快照】： 肝臟缺血再灌注損傷是梗阻性黃疸手術(shù)中常見的病理生理過程,其產(chǎn)生原因與原發(fā)疾病本身及手術(shù)方式等有關(guān),可導(dǎo)致肝臟本身及遠(yuǎn)隔臟器(如腎臟)的損傷[1~3]。氧化應(yīng)激和炎癥反應(yīng)是缺血再灌注損傷的主要發(fā)病機(jī)制。麻醉劑七氟醚具有抗炎特性,但其對缺血再灌注損傷的影響尚不清楚。20

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 施養(yǎng)德,秦劍，

本文編號：1395469