發(fā)布時間：2019-05-27 14:15

【摘要】：目的：構建BBF在耳蝸植入術后感染動物體內(nèi)模型，及形態(tài)學觀察；觀察表皮葡萄球菌的體內(nèi)外BBF的形成、結構形態(tài)模型；探討臨床上清除及抑制細菌生物膜形成的相關治療措施。 方法：建立表皮葡萄球菌生物膜的體外模型，利用掃描電鏡觀察新西蘭兔CI術后體內(nèi)BBF感染模型。 結果：①成功建立了表皮葡萄球菌的體外生物膜，用剛果紅培養(yǎng)基對BBF檢測進行初篩；②成功構建耳蝸植入術后感染新西蘭兔體內(nèi)模型；③掃描電鏡下成功觀察到典型的BBF形態(tài)結構，，細菌間纖維樣物質(zhì)相互粘連，細菌形態(tài)，球形的表皮葡萄球菌成團分布、聚集，呈多層細胞結構，纖維樣粘多糖和菌體間的連結，菌絲、細胞外基質(zhì)（ECM）相互交錯，形成致密的膜網(wǎng)狀空間結構；④臨床上，經(jīng)過綜合處理，采用2步驟的方法，使用化學制劑如H2O2、濃碘伏可有效清除和抑制細菌生物膜的形成。 結論：①本實驗研究結果觀察到了表皮葡萄球菌生物膜體外模型的結構，對其表型進行初步檢測，了解其生長動力學，證明了表皮葡萄球菌生物膜具有強大的致病性；②新西蘭兔體內(nèi)生物膜的形態(tài)得到證實，通過掃描電鏡下可以直觀的觀察到人工耳蝸植入術后感染新西蘭兔植入體周圍S.E.生物膜的結構，為進一步研究人工耳蝸植入術后感染提供了實驗的模型和支持；③臨床上利用細菌生物膜清除劑有3%過氧化氫溶液、碘伏等，可有效清除和抑制細菌生物膜的形成。
[Abstract]:Objective: to construct the in vivo model and morphological observation of BBF in infected animals after cochlear implantation, and to observe the formation and structural morphology model of BBF in vitro and in vivo of Staphylococcus epidermidis. To explore the clinical treatment measures to clear and inhibit the formation of bacterial biofilm. Methods: the in vitro model of Staphylococcus epidermidis biofilm was established. The model of BBF infection in New Zealand rabbits after CI was observed by scanning electron microscope (SEM). Results: (1) the biofilm of Staphylococcus epidermidis in vitro was successfully established and screened for BBF with Congo red culture medium, and (2) the model of infected New Zealand rabbits after cochlear implantation was successfully constructed. (3) the typical morphology and structure of BBF were successfully observed under scanning electron microscope (SEM). The fiber-like substances adhered to each other, the bacterial morphology, the cluster distribution and aggregation of spherical Staphylococcus epidermidis, and the connection between fibroid mucopolysaccharide and bacteria. The hyphae and extracellular matrix (ECM) interlaced with each other to form a dense membrane reticular spatial structure. (4) after comprehensive treatment and two-step method, concentrated iodine volt can effectively eliminate and inhibit the formation of bacterial biofilm by using chemical preparations such as H _ 2O _ 2. Conclusion: 1 the structure of Staphylococcus epidermidis biofilm model in vitro was observed, the phenotype of Staphylococcus epidermidis biofilm was detected, the growth kinetics of Staphylococcus epidermidis biofilm was understood, and it was proved that Staphylococcus epidermidis biofilm had strong pathogenicity. 2 the morphology of biofilm in New Zealand rabbits was confirmed. The infection around the implantation of New Zealand rabbits after cochlear implantation could be observed intuitively by scanning electron microscope. The structure of biofilm provides experimental model and support for further study of infection after cochlear implantation. (3) there are 3% hydrogen peroxide solution, iodophor and so on, which can effectively eliminate and inhibit the formation of bacterial biofilm by using bacterial biofilm remover.
【學位授予單位】：福建醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R764.93

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