【摘要】：目的：構(gòu)建BBF在耳蝸植入術(shù)后感染動(dòng)物體內(nèi)模型，及形態(tài)學(xué)觀察；觀察表皮葡萄球菌的體內(nèi)外BBF的形成、結(jié)構(gòu)形態(tài)模型；探討臨床上清除及抑制細(xì)菌生物膜形成的相關(guān)治療措施。 方法：建立表皮葡萄球菌生物膜的體外模型，利用掃描電鏡觀察新西蘭兔CI術(shù)后體內(nèi)BBF感染模型。 結(jié)果：①成功建立了表皮葡萄球菌的體外生物膜，用剛果紅培養(yǎng)基對(duì)BBF檢測(cè)進(jìn)行初篩；②成功構(gòu)建耳蝸植入術(shù)后感染新西蘭兔體內(nèi)模型；③掃描電鏡下成功觀察到典型的BBF形態(tài)結(jié)構(gòu)，，細(xì)菌間纖維樣物質(zhì)相互粘連，細(xì)菌形態(tài)，球形的表皮葡萄球菌成團(tuán)分布、聚集，呈多層細(xì)胞結(jié)構(gòu)，纖維樣粘多糖和菌體間的連結(jié)，菌絲、細(xì)胞外基質(zhì)（ECM）相互交錯(cuò)，形成致密的膜網(wǎng)狀空間結(jié)構(gòu)；④臨床上，經(jīng)過(guò)綜合處理，采用2步驟的方法，使用化學(xué)制劑如H2O2、濃碘伏可有效清除和抑制細(xì)菌生物膜的形成。 結(jié)論：①本實(shí)驗(yàn)研究結(jié)果觀察到了表皮葡萄球菌生物膜體外模型的結(jié)構(gòu)，對(duì)其表型進(jìn)行初步檢測(cè)，了解其生長(zhǎng)動(dòng)力學(xué)，證明了表皮葡萄球菌生物膜具有強(qiáng)大的致病性；②新西蘭兔體內(nèi)生物膜的形態(tài)得到證實(shí)，通過(guò)掃描電鏡下可以直觀的觀察到人工耳蝸植入術(shù)后感染新西蘭兔植入體周圍S.E.生物膜的結(jié)構(gòu)，為進(jìn)一步研究人工耳蝸植入術(shù)后感染提供了實(shí)驗(yàn)的模型和支持；③臨床上利用細(xì)菌生物膜清除劑有3%過(guò)氧化氫溶液、碘伏等，可有效清除和抑制細(xì)菌生物膜的形成。
[Abstract]:Objective: to construct the in vivo model and morphological observation of BBF in infected animals after cochlear implantation, and to observe the formation and structural morphology model of BBF in vitro and in vivo of Staphylococcus epidermidis. To explore the clinical treatment measures to clear and inhibit the formation of bacterial biofilm. Methods: the in vitro model of Staphylococcus epidermidis biofilm was established. The model of BBF infection in New Zealand rabbits after CI was observed by scanning electron microscope (SEM). Results: (1) the biofilm of Staphylococcus epidermidis in vitro was successfully established and screened for BBF with Congo red culture medium, and (2) the model of infected New Zealand rabbits after cochlear implantation was successfully constructed. (3) the typical morphology and structure of BBF were successfully observed under scanning electron microscope (SEM). The fiber-like substances adhered to each other, the bacterial morphology, the cluster distribution and aggregation of spherical Staphylococcus epidermidis, and the connection between fibroid mucopolysaccharide and bacteria. The hyphae and extracellular matrix (ECM) interlaced with each other to form a dense membrane reticular spatial structure. (4) after comprehensive treatment and two-step method, concentrated iodine volt can effectively eliminate and inhibit the formation of bacterial biofilm by using chemical preparations such as H _ 2O _ 2. Conclusion: 1 the structure of Staphylococcus epidermidis biofilm model in vitro was observed, the phenotype of Staphylococcus epidermidis biofilm was detected, the growth kinetics of Staphylococcus epidermidis biofilm was understood, and it was proved that Staphylococcus epidermidis biofilm had strong pathogenicity. 2 the morphology of biofilm in New Zealand rabbits was confirmed. The infection around the implantation of New Zealand rabbits after cochlear implantation could be observed intuitively by scanning electron microscope. The structure of biofilm provides experimental model and support for further study of infection after cochlear implantation. (3) there are 3% hydrogen peroxide solution, iodophor and so on, which can effectively eliminate and inhibit the formation of bacterial biofilm by using bacterial biofilm remover.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R764.93

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