【摘要】：目的探討姜黃素對(duì)紫外線A(ultraviolet A,UVA)照射小鼠角膜損傷的抑制作用。方法取C57 BL/6小鼠30只,隨機(jī)分為3組,每組各10只;UVA照射組雙眼接受位于前上方的UVA照射,波長(zhǎng)為320~400 nm,照射強(qiáng)度為0.05 W·cm~(-2),照射距離固定,照射時(shí)間24 h。姜黃素干預(yù)組在UVA照射前3 d給予小鼠姜黃素30 mg·kg~(-1)腹腔內(nèi)注射,并持續(xù)至照射后48 h。對(duì)照組不做任何處理。利用裂隙燈觀察角膜病變并對(duì)角膜混濁進(jìn)行分級(jí);照射后48 h取小鼠角膜組織,使用電泳遷移率實(shí)驗(yàn)檢測(cè)核轉(zhuǎn)錄因子-κB(nuclear factor-κB,NF-κB)的表達(dá)。結(jié)果照射后48 h,UVA照射組角膜混濁臨床評(píng)分(3.10±0.74)均高于對(duì)照組(0)及姜黃素干預(yù)組(0.35±0.24)。兩兩比較發(fā)現(xiàn)對(duì)照組與姜黃素干預(yù)組間角膜混濁臨床評(píng)分差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);UVA照射組與對(duì)照組以及姜黃素干預(yù)組間差異均有統(tǒng)計(jì)學(xué)意義(均為P0.05)。照射后48 h,角膜組織NF-κB表達(dá)水平對(duì)照組(1.25±0.13)與姜黃素干預(yù)組(1.58±0.34)均較低,UVA照射組(3.98±0.58)明顯升高。兩兩比較發(fā)現(xiàn)對(duì)照組與姜黃素干預(yù)組角膜組織NF-κB表達(dá)水平差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);UVA照射組與對(duì)照組以及姜黃素干預(yù)組間差異均有統(tǒng)計(jì)學(xué)意義(均為P0.05)。結(jié)論姜黃素可以減輕UVA照射對(duì)小鼠角膜造成的損傷,其作用可能是通過(guò)抑制角膜組織內(nèi)NF-κB表達(dá)實(shí)現(xiàn)的。
[Abstract]:Objective to investigate the inhibitory effect of curcumin on corneal injury induced by ultraviolet A (UVA) irradiation in mice. Methods Thirty C57 BL/6 mice were randomly divided into three groups with 10 mice in each group. In the UVA group, the eyes were exposed to the anterior-superior UVA irradiation. The irradiation intensity was 0.05 W cm~ (- 2) at the wavelength of 320 渭 400 nm,. The irradiation distance was fixed and the irradiation time was 24 h. Curcumin intervention group was injected intraperitoneally with curcumin 30 mg kg~ (- 1) 3 days before UVA irradiation and lasted until 48 hours after irradiation. The control group did not do any treatment. The keratopathy was observed by slit lamp and the corneal opacity was graded, and the expression of nuclear factor-魏 B (nuclear factor- kappa B (NF- 魏 B) was detected by electrophoretic mobility shift assay (EMSA) 48 h after irradiation. Results 48 hours after irradiation, the clinical score of corneal opacification in UVA irradiation group (3.10 鹵0.74) was higher than that in control group (0) and curcumin intervention group (0.35 鹵0.24). There was no significant difference in the clinical score of corneal opacification between the control group and curcumin intervention group (P0.05) between the); UVA irradiation group and the control group and curcumin intervention group (P0.05). 48 hours after irradiation, the expression level of NF- 魏 B in corneal tissue was lower in control group (1.25 鹵0.13) and curcumin intervention group (1.58 鹵0.34), while that in UVA group was significantly higher than that in control group (3.98 鹵0.58). Pairwise comparison showed that there was no significant difference in the expression of NF- 魏 B between the control group and curcumin intervention group (P0.05) between the); UVA irradiation group and the control group and curcumin intervention group (P0.05). Conclusion curcumin can reduce the corneal damage induced by UVA irradiation in mice, which may be achieved by inhibiting the expression of NF- 魏 B in corneal tissue.
【作者單位】： 南京大學(xué)醫(yī)學(xué)院臨床學(xué)院南京總醫(yī)院眼科;
【基金】：南京總醫(yī)院課題(編號(hào):2016007)~~
【分類(lèi)號(hào)】：R77

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