【摘要】：目的研究纖維蛋白膠羊膜棒對去勢雄兔干眼癥的預(yù)防和治療作用。方法選取雄性新西蘭大白兔60只(60眼,均為右眼),行雙側(cè)睪丸切除術(shù)后隨機(jī)分為A、B、C、D、E及F 6組(各組10眼)。A組手術(shù)后不進(jìn)行任何處理觀察6周;B組手術(shù)當(dāng)日將纖維蛋白膠羊膜棒植入淚道5 s后取出觀察6周;C組手術(shù)當(dāng)日植入纖維蛋白膠羊膜棒6周;D組造模(手術(shù)2個月后形成干眼模型)后不進(jìn)行任何處理觀察6周;E組造模當(dāng)日將纖維蛋白膠羊膜棒植入淚道5 s后取出觀察6周;F組造模當(dāng)日植入纖維蛋白膠羊膜棒6周。分別于植入前和植入后2、4及6周行Schirmer I試驗(SIT)、角膜熒光素染色(FL)、角膜共聚焦顯微鏡掃描和淚道黏膜K16的Western blot檢查。結(jié)果 C組在植入2、4及6周后SIT較植入之前好轉(zhuǎn),差異有統(tǒng)計學(xué)意義(P0.05);C組與A、B組間的SIT在各時間比較,差異有統(tǒng)計學(xué)意義(P0.05);在植入2周后,C組FL指標(biāo)評分較植入之前無明顯變化,差異無統(tǒng)計學(xué)意義(P0.05);在植入4和6周后C組與A、B組FL指標(biāo)評分較植入之前輕度好轉(zhuǎn),差異有統(tǒng)計學(xué)意義(P0.05)。在植入2、4及6周后,F組SIT較植入前好轉(zhuǎn),差異有統(tǒng)計學(xué)意義(P0.05),F組FL指標(biāo)評分較植入之前好轉(zhuǎn),差異有統(tǒng)計學(xué)意義(P0.05);植入后C、F組上皮基底細(xì)胞和炎癥細(xì)胞均減少,差異有統(tǒng)計學(xué)意義(P0.05)。免疫熒光染色及Western blot結(jié)果顯示C、F組淚道黏膜K16陰性表達(dá)。結(jié)論纖維蛋白膠羊膜棒能夠有效地預(yù)防和治療去勢雄兔干眼癥,延緩干眼癥的進(jìn)展。
[Abstract]:Objective to study the preventive and therapeutic effects of fibrin gel amnion rod on dry eye disease in castrated male rabbits. Methods 60 male New Zealand white rabbits (60 eyes, all right eyes) were randomly divided into A, B, C, D, E and F 6 groups after bilateral orchiectomy (10 eyes in each group were treated with). A without any treatment for 6 weeks). The fibrin glue amniotic rod was implanted into the lacrimal duct for 6 weeks, and the fibrin glue amniotic rod was implanted for 6 weeks on the day of operation in group B, and the fibrin glue amniotic rod was implanted in group C for 6 weeks. The model of group D (dry eye model was formed 2 months after operation) was observed without any treatment for 6 weeks, and the fibrin glue amniotic rod was implanted into lacrimal duct for 6 weeks on the same day of modeling in group E. The rats in group E were taken out for 6 weeks after implantation of fibrin gel into lacrimal duct. Group F was implanted with fibrin gel amnion rod for 6 weeks. Before and 2, 4 and 6 weeks after implantation, Schirmer I test (SIT), fluorescein stained (FL), corneal confocal microscope and lacrimal duct mucosa K16 Western blot were performed. Results after 2 weeks, 4 weeks and 6 weeks after implantation, SIT in group C was better than that before implantation, the difference was statistically significant (P0.05) between); C group and A, B group in each time comparison, the difference was statistically significant (P0.05); 2 weeks after implantation, there was no significant difference in FL score between group C and group C (P0.05). After 4 weeks and 6 weeks after implantation, the FL scores of group C and group A, B were slightly improved compared with those before implantation, the difference was statistically significant (P0.05). After 2 weeks, 4 weeks and 6 weeks after implantation, the SIT in group F was better than that before implantation, the difference was statistically significant (P0.05), the FL score in group), F was better than that before implantation, the difference was statistically significant (P0.05). After implantation C, F group epithelial basal cells and inflammatory cells decreased, the difference was statistically significant (P0.05). Immunofluorescence staining and Western blot showed negative expression of K16 in lacrimal duct mucosa of C and F groups. Conclusion fibrin gel amnion rod can effectively prevent and treat dry eye disease in ovariectomized male rabbits, and delay the progress of dry eye disease in ovariectomized male rabbits.
【作者單位】： 南昌大學(xué)第一附屬醫(yī)院眼科;南華大學(xué)第一附屬醫(yī)院眼科;廈門大學(xué)眼科研究所;
【基金】：國家自然科學(xué)基金(No:81160118,81400372,81400424,81660158) 江西省遠(yuǎn)航工程(No:2014022) 江西省自然科學(xué)基金重大項目(No:2016ACB21017) 江西省青年科學(xué)基金(No:20151BAB215016) 江西省科技支撐計劃項目(No:20151BBG70223)
【分類號】：R777.34

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