甲狀腺相關(guān)眼病患者外周血單個(gè)核細(xì)胞中微小RNA-146a的異常表達(dá)及意義
發(fā)布時(shí)間：2019-03-16 10:27
【摘要】：目的探討微小RNA-146a (microRNA-146a, miRNA-146a, miR-146a)在甲狀腺相關(guān)眼病(TAO)患者外周血單個(gè)核細(xì)胞中的表達(dá)情況及臨床意義,為TAO的治療提供新思路。 方法收集TAO患者、Gaves病(GD)不伴眼病患者和正常人對(duì)照者空腹靜脈全血標(biāo)本各20例,分離各組患者外周血單個(gè)核細(xì)胞(PBMCs),用TRIzol提取PBMCs的總miRNA,再以頸環(huán)結(jié)構(gòu)的引物逆轉(zhuǎn)錄出相應(yīng)的DNA,然后以miR-146a的PCR引物進(jìn)行實(shí)時(shí)熒光定量PCR(qRT-PCR)檢測(cè)各組標(biāo)本PBMCs中miR-146a的表達(dá)情況,電泳觀察結(jié)果. 結(jié)果與正常人相比,TAO患者和GD不伴眼病患者PBMCs中miR-146a相對(duì)表達(dá)量明顯降低(P0.05),下調(diào)率分別為98.02%和67.60%；較GD不伴眼病患者,miR-146a在TAO患者PBMCs中相對(duì)表達(dá)量更低(P0.05),下調(diào)率差值為30.40%。 結(jié)論1.基于SYBR Green Ⅰ染料熒光分析的莖環(huán)qRT-PCR方法檢測(cè)miR-146a表達(dá)的特異性良好； 2、miR-146a的表達(dá)下調(diào)可能參與了TAO的發(fā)病機(jī)制,為進(jìn)一步研究微小RNA在TAO發(fā)病機(jī)制中的作用打下基礎(chǔ)。
[Abstract]:Objective To study the expression and clinical significance of microRNA-146a (microRNA-146a, miRNA-146a, miR-146a) in peripheral blood mononuclear cells of patients with thyroid-associated ophthalmopathy (TAO) and to provide a new thought for the treatment of TAO. Methods 20 patients with TAO, Gaves disease (GD) and normal controls were collected, and the peripheral blood mononuclear cells (PBMCs) were isolated and the total miRNAs of PBMCs were extracted with TRIzol. A. The expression of miR-146a in PBMCs of each group was detected by real-time fluorescence quantitative PCR (qRT-PCR) using the PCR primer of miR-146a. Results Compared with normal subjects, the relative expression of miR-146a in patients with TAO and GD was significantly lower than that of normal subjects (P0.05). The down-regulation rate was 98.02% and 67.60%, respectively. The relative expression of miR-146a in PBMCs of patients with GD was lower (P0.05), and the difference of down-regulation was 30. 40%. Conclusion 1. The expression of miR-146a is detected by the stem-loop qRT-PCR method based on the fluorescence analysis of SYBR Green I dye. Good specificity; the down-regulation of miR-146a may be involved in the pathogenesis of TAO, in order to further study the mechanism of small RNA in the pathogenesis of TAO
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R771.3
本文編號(hào)：2441171
[Abstract]:Objective To study the expression and clinical significance of microRNA-146a (microRNA-146a, miRNA-146a, miR-146a) in peripheral blood mononuclear cells of patients with thyroid-associated ophthalmopathy (TAO) and to provide a new thought for the treatment of TAO. Methods 20 patients with TAO, Gaves disease (GD) and normal controls were collected, and the peripheral blood mononuclear cells (PBMCs) were isolated and the total miRNAs of PBMCs were extracted with TRIzol. A. The expression of miR-146a in PBMCs of each group was detected by real-time fluorescence quantitative PCR (qRT-PCR) using the PCR primer of miR-146a. Results Compared with normal subjects, the relative expression of miR-146a in patients with TAO and GD was significantly lower than that of normal subjects (P0.05). The down-regulation rate was 98.02% and 67.60%, respectively. The relative expression of miR-146a in PBMCs of patients with GD was lower (P0.05), and the difference of down-regulation was 30. 40%. Conclusion 1. The expression of miR-146a is detected by the stem-loop qRT-PCR method based on the fluorescence analysis of SYBR Green I dye. Good specificity; the down-regulation of miR-146a may be involved in the pathogenesis of TAO, in order to further study the mechanism of small RNA in the pathogenesis of TAO
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R771.3
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 崔慧娟;唐元家;羅曉兵;鄧筠;郭彥芝;黃新芳;陳順樂(lè);沈南;;系統(tǒng)性紅斑狼瘡患者中miR-146a表達(dá)缺陷與I型干擾素通路過(guò)度活化相關(guān)[J];現(xiàn)代免疫學(xué);2008年04期
，本文編號(hào)：2441171
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