【摘要】：目的： 制作常壓下慢性間歇性缺氧的大鼠模型，以探討CIH與胃食管反流病的關(guān)系及CIH對(duì)食管白細(xì)胞介素-6（Interleukin-6,IL-6）,超敏c-反應(yīng)蛋白（high-sensitivity C-reactive protein,hs-CRP）表達(dá)的影響。 方法: 將24只成年雄性SD大鼠隨機(jī)分成3組，即空白對(duì)照組(unhandledcontrol group，UC組，按夜間進(jìn)食、水，而白天禁食、水，不限制活動(dòng)，不加任何人工低氧干預(yù)的方案飼養(yǎng)4周），慢性間歇性缺氧組（chronic intermittent hypoxia group，CIH組，按UC組方法喂養(yǎng)，連續(xù)4周給予每天白天8h的間歇性缺氧）和復(fù)氧組（Removal of hypoxiagroup，RH組，按CIH方法喂養(yǎng)4周后再按UC組方法飼養(yǎng)4周），所有大鼠均在按規(guī)定完成實(shí)驗(yàn)后測(cè)定各組食管下段PH值，再在肉眼及光鏡下觀(guān)察食管病理變化，，用酶聯(lián)免疫法檢測(cè)食管組織IL-6及hs-CRP水平。 結(jié)果: 1.CIH組食管下段PH（potential of hydrogen）值較UC組明顯下降(p0.05)，RH組與UC組無(wú)明顯差異； 2. CIH組食管發(fā)生明顯炎癥改變，UC組和RH組未發(fā)現(xiàn)明顯的炎癥性改變； 3. CIH組IL-6及hs-CRP的表達(dá)明顯增高(P0.01)，RH組與UC組比較無(wú)統(tǒng)計(jì)學(xué)差異(p0.05)。 結(jié)論: CIH可使大鼠食管PH值下降，食管炎性改變明顯，IL-6及hs-CRP表達(dá)升高，復(fù)氧可使其恢復(fù)。
[Abstract]:Objective: to study the relationship between CIH and gastroesophageal reflux disease (GERD) and the effect of CIH on esophageal interleukin-6 (Interleukin-6,IL-6) and high-sensitivity C-reactive protein, in rats with chronic intermittent hypoxia under normal pressure. Hs-CRP) expression. Methods: Twenty-four adult male SD rats were randomly divided into three groups: control group (unhandledcontrol group,UC group, fed with night-time food, water, daytime fasting, water, no restriction of activity, and no artificial hypoxic intervention for 4 weeks). The chronic intermittent hypoxia group (chronic intermittent hypoxia group,CIH group) was fed with UC for 4 weeks, and the reoxygenation group (Removal of hypoxiagroup,RH group) and the reoxygenation group (Removal of hypoxiagroup,RH group) were fed with CIH method for 4 weeks and then fed with UC group for 4 weeks). The PH values of lower esophagus in all groups were measured after completion of the experiment. The pathological changes of esophagus were observed with naked eye and light microscope. The levels of IL-6 and hs-CRP in esophageal tissue were detected by enzyme-linked immunosorbent assay (Elisa). Results: the PH (potential of hydrogen) value of lower esophagus in 1.CIH group was significantly lower than that in UC group (p0.05), RH group and UC group had no significant difference; 2. In CIH group, there were obvious inflammatory changes in esophagus, but no obvious inflammatory changes were found in UC group and RH group. The expression of IL-6 and hs-CRP in CIH group was significantly higher than that in UC group (P 0.01). Conclusion: CIH can decrease the value of PH in rat esophagus, increase the expression of IL-6 and hs-CRP, and reoxygenate can make it recover.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R766

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