【摘要】：目的:研究紅芪多糖對內(nèi)毒素誘導(dǎo)的大鼠葡萄膜炎TLR-4(Toll樣受體-4)信號轉(zhuǎn)導(dǎo)通路中下游信號轉(zhuǎn)導(dǎo)通路元件TRAF6(腫瘤壞死因子受體相關(guān)因子-6)及TRIF(Toll樣受體活化相關(guān)因子)表達(dá)的影響,探討紅芪多糖抑制內(nèi)毒素誘導(dǎo)的大鼠葡萄膜炎可能的作用機(jī)制。方法:實(shí)驗(yàn)選用Wistar大鼠作為研究對象,采用霍亂弧菌內(nèi)毒素1mg/kg皮下注射的方法,建立大鼠急性前葡萄膜炎動物模型。Wistar大鼠160只被隨機(jī)分為4組,每組40只:空白對照組,EIU模型組,LPS+HPS組(內(nèi)毒素+紅芪多糖組)和HPS組(紅芪多糖組)。LPS+HPS組及空白對照組大鼠分別在內(nèi)毒素注射前1小時給予腹腔注射紅芪多糖400 mg/kg及等量磷酸鹽緩沖液。HPS組大鼠只注射紅芪多糖400 mg/kg。每2小時用裂隙燈觀察大鼠眼前節(jié)炎癥反應(yīng)并評分。組織病理學(xué)檢查觀察炎癥反應(yīng)水平。實(shí)時定量PCR檢測TRAF6及TRIF m RNA的表達(dá)。用蛋白免疫印跡法從蛋白水平檢測TRAF6及TRIF的表達(dá)。結(jié)果:成功建立急性前葡萄膜炎動物模型。LPS+HPS組大鼠眼前節(jié)炎癥反應(yīng)及組織病理學(xué)炎癥表現(xiàn)明顯減輕。實(shí)時定量PCR結(jié)果顯示紅芪多糖可抑制TRAF6m RNA的表達(dá),抑制其蛋白的降解,紅芪多糖對TRIF的作用不明顯。結(jié)論:紅芪多糖可以通過抑制TRAF6核酸的表達(dá)和蛋白的降解緩解內(nèi)毒素誘導(dǎo)的大鼠急性前葡萄膜炎眼內(nèi)炎癥反應(yīng)。紅芪多糖對EIU模型的干預(yù)途徑主要是通過阻斷TLR4-My D88依賴途徑而發(fā)揮的作用。
[Abstract]:Objective: to study the effects of hongqi polysaccharide on endotoxin-induced uveitis TLR-4 (Toll like receptor 4) signal transduction pathway element TRAF6 (tumor necrosis factor receptor related factor 6) and TRIF ( The expression of Toll like receptor activation-related factors, To explore the possible mechanism of hongqi polysaccharide inhibiting endotoxin induced uveitis in rats. Methods: Wistar rats were selected as the research object. The animal model of acute anterior uveitis was established by subcutaneous injection of vibrio cholerae endotoxin 1mg/kg. 160 Wistar rats were randomly divided into 4 groups, 40 rats in each group: blank control group. EIU model group, Rats in LPS HPS group (lipopolysaccharide group) and HPS group (Hongqi polysaccharide group). LPS HPS group and blank control group) were given intraperitoneal injection of Hongqi polysaccharide 400 mg/kg and the same amount of phosphate buffer 1 hour before endotoxin injection, respectively. Liquid. Rats in HPS group were injected with polysaccharides of Rhizoma membranaceus for 400 mg/kg.. The inflammatory reaction of rat anterior segment was observed by slit lamp every 2 hours. The level of inflammatory reaction was observed by histopathological examination. The expression of TRAF6 and TRIF m RNA was detected by real-time quantitative PCR. The expression of TRAF6 and TRIF was detected by Western blot at protein level. Results: the inflammatory reaction and histopathological inflammation of anterior uveitis in. LPS HPS group were alleviated obviously. The results of real-time quantitative PCR showed that the polysaccharides of Hongqi could inhibit the expression of TRAF6m RNA and the degradation of its protein, but the effect of polysaccharides on TRIF was not obvious. Conclusion: hongqi polysaccharide can alleviate the intraocular inflammation induced by endotoxin in rats with acute anterior uveitis by inhibiting the expression of TRAF6 nucleic acid and protein degradation. The intervention pathway of APS on EIU model was mainly by blocking the TLR4-My D88 dependent pathway.
【學(xué)位授予單位】：首都醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R773

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張曉龍;王婧;許卓再;李中秋;盧弘;;大黃多糖對內(nèi)毒素誘導(dǎo)急性前葡萄膜炎TLR4/NF-κB傳導(dǎo)通路干預(yù)作用的實(shí)驗(yàn)研究[J];眼科;2013年02期

2 朱明瓊;鄺國平;;白介素-1與眼底病變[J];現(xiàn)代醫(yī)藥衛(wèi)生;2013年02期

3 滿輝;黃旭東;黃靜;;內(nèi)毒素誘導(dǎo)葡萄膜炎中炎癥細(xì)胞凋亡與TRAIL表達(dá)的研究[J];國際眼科雜志;2013年01期

4 王少程;葛慶曼;林錦鏞;鄭曰忠;;白細(xì)胞介素-10對內(nèi)毒素誘導(dǎo)的實(shí)驗(yàn)性葡萄膜炎的治療作用[J];中華眼底病雜志;2010年05期

5 李勁;宋琳;彭偉;朱格非;胡慧麗;聶愛芹;;腫瘤壞死因子α反義寡核苷酸對內(nèi)毒素誘導(dǎo)的葡萄膜炎的抑制作用[J];華中科技大學(xué)學(xué)報(醫(yī)學(xué)版);2010年04期

6 李上;盧弘;胡小鳳;陳巍;楊培增;Aize Kijlstra;許穎知;王婧;;TLR4-MyD88在大鼠急性前葡萄膜炎虹膜中的表達(dá)[J];眼科研究;2010年02期

7 翁欣瑜;吳群;;NF-κB阻斷劑對內(nèi)毒素致大鼠葡萄膜炎中TLR-4表達(dá)的影響[J];眼科新進(jìn)展;2009年12期

8 張瑋;盧弘;華文;李學(xué)東;;內(nèi)毒素誘導(dǎo)的大鼠葡萄膜炎細(xì)胞凋亡的研究[J];眼科研究;2009年05期

9 劉端勇;趙海梅;周楓;黃小英;呂愛平;羅曉建;;黃芪多糖調(diào)節(jié)小鼠小腸黏膜淋巴細(xì)胞因子的表達(dá)[J];中國中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志;2008年09期

10 馬丹;封士蘭;趙良功;劉小花;李冰;李曉東;胡芳弟;;紅芪多糖的提取分離純化及組成分析[J];中國現(xiàn)代應(yīng)用藥學(xué);2008年03期

，

本文編號：2395529