【摘要】：目的：探討正常以及視神經(jīng)切斷后成年大鼠視盤周圍神經(jīng)纖維層(retinal nerve fiber layer, RNFL)厚度的光學相干斷層掃描(optical coherence tomography, OCT)測量與組織學測量結(jié)果的相關(guān)性。 方法：采用OCT對36只大鼠(正常組20只；視神經(jīng)切斷模型組16只,分為1,3,5和7天存活組,每組4只)進行以視乳頭為中心直徑3.46mm圓環(huán)區(qū)域的視盤周圍RNFL的厚度掃描,然后進行同一視網(wǎng)膜NF-200的免疫組織化學染色,測量相應部位的RNFL厚度。分別對西種方法測量的正常和模型組大鼠RNFL厚度數(shù)據(jù)進行線性回歸統(tǒng)計分析。 結(jié)果：OCT檢測正常大鼠視盤周轉(zhuǎn)RNFL厚度,右眼為72.35±5.71μm、左眼為72.65±5.88μm,雙眼間差異無統(tǒng)計學意義(p0.05)；組織切片測量正常大鼠視盤周圍RNFL厚度,右眼為37.54±4.05μm、左眼為37.38±4.23μm,雙眼間差異無統(tǒng)計學意義(p0.05)；兩種測量方法結(jié)果顯示相關(guān)性較好(R2=0.8131)。在視神經(jīng)切斷模型組,兩種方法的測量結(jié)果均顯示視神經(jīng)切斷后大鼠視盤周圍RNFL厚度值具有隨著大鼠存活時間延長逐漸變小的趨勢,且兩種測量方法結(jié)果具有較好的相關(guān)性(R2=0.7964)。OCT測得的正常大鼠以及模型組大鼠視盤周圍RNFL厚度明顯高于組織學測量值。 結(jié)論：采用OCT檢測的視網(wǎng)膜神經(jīng)纖維層厚度值可以代表該部位厚度的相對值,并且該厚度值與通過組織學方法獲得的視網(wǎng)膜神經(jīng)纖維層厚度值相關(guān)性很好。
[Abstract]:Aim: to investigate the correlation between optical coherence tomography (optical coherence tomography, OCT) and histological measurements of (retinal nerve fiber layer, RNFL) thickness of perioptic nerve fiber layer in normal and adult rats after optic nerve transection. Methods: 36 rats (20 normal rats) were treated with OCT. Sixteen rats in the optic nerve transection model group were divided into 5 and 7 days survival group (4 rats in each group). The thickness of RNFL around the optic disc with the optic papilla as the central diameter of the 3.46mm circle was scanned. The thickness of RNFL was measured by immunohistochemical staining of the same retinal NF-200. The RNFL thickness data of normal and model rats measured by Western method were analyzed by linear regression. Results: the thickness of RNFL was 72.35 鹵5.71 渭 m in the right eye and 72.65 鹵5.88 渭 m in the left eye. There was no significant difference between the two eyes (p0.05). The thickness of RNFL around optic disc in normal rats was 37.54 鹵4.05 渭 m in right eye and 37.38 鹵4.23 渭 m in left eye. In the optic nerve transection model group, the results of the two methods showed that the thickness of RNFL around the optic disc of the rats after optic nerve transection had a tendency to decrease with the prolongation of the survival time of the rats. There was a good correlation between the results of the two methods (the thickness of RNFL around the optic disc of normal rats and model rats measured by R2 + 0.7964). OCT) was significantly higher than that of histological measurement. Conclusion: the thickness of retinal nerve fiber layer measured by OCT can represent the relative value of the thickness of the retinal nerve fiber layer, and it has a good correlation with the thickness of retinal nerve fiber layer obtained by histological method.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R774.6

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