飽和氫氣生理鹽水對大鼠視網(wǎng)膜藍(lán)光損傷的保護(hù)作用
發(fā)布時間：2018-10-13 17:02
【摘要】：目的：視網(wǎng)膜光損傷,主要與活性氧(ROS)的堆積有關(guān),是ARMD、RP及其他多種視網(wǎng)膜疾病重要機(jī)制。氫氣是一種選擇性的抗氧化劑,氫氣溶解在液體中可選擇性中和羥自由基和過氧亞硝酸陰離子,而后兩者是氧化損傷最重要的介質(zhì)。本實驗中,我們探討飽和氫氣生理鹽水溶液對大鼠視網(wǎng)膜藍(lán)光損傷的作用。 方法：取100只健康雌性大鼠(160g-180g),隨機(jī)分為四組。四組大鼠飼養(yǎng)于12H明12H暗環(huán)境中,并可自由攝取飲食。造模期間,一組不做處理,二組及三組按照大鼠體重分別予以1ml/100g生理鹽水和飽和氫氣生理鹽水腹腔注射,四組作為對照組。二十天后,以藍(lán)光照射6小時建立大鼠視網(wǎng)膜光損傷模型。于光照后8小時,16小時和24小時檢測視網(wǎng)膜相關(guān)指標(biāo)。樣本取材后,通過H-E染色,超微結(jié)構(gòu)觀察,生化指標(biāo)檢測,觀察視網(wǎng)膜損傷程度及飽和氫氣生理鹽水的作用。 結(jié)果：通過H-E檢測發(fā)現(xiàn),一組和二組視網(wǎng)膜結(jié)構(gòu)損傷重,三組損傷輕。一組與二組間無明顯差異。與一組和二組相比,三組的視網(wǎng)膜結(jié)構(gòu)層次清晰,細(xì)胞排列整齊。400倍鏡下可見,除了一組與二組之間,不同時間點各組之間的外核層厚度比較有明顯差異(p0.05)。三個時間點,一組的外核層厚度為30.41±4.04μm,26.11±2.82μm和20.63±1.06μm,二組為31.62±4.5μm,25.08±3.63μm和19.07±3.86μm,三組為29.75±3.62μm,28.83±1.97μm和27.61±1.813μm,四組的平均厚度為37.35±1.37μmm。由此得出,隨著時間延長,損傷不斷加重。在24小時時,損傷的差別最大。與四組相比,一組薄46.23%,二組薄50.29%,三組薄28.04%。電鏡下可見,三組損傷明顯較一組和二組輕。在各時間點與四組相比,一組內(nèi)二醛的含量明顯增高(p0.05)。三組丙二醛含量明顯低于一組(p0.05)和二組(p0.05)。在一組和二組之間比較,各時間點無明顯差異(p0.05)。 結(jié)論：檢測的結(jié)果顯示,飽和氫氣生理鹽水能有效的保護(hù)視網(wǎng)膜,減輕光損傷,這種保護(hù)作用跟氫氣的抗氧化作用有關(guān)。這些發(fā)現(xiàn),使氫氣成為治療ARMD等光損傷導(dǎo)致的氧化應(yīng)激相關(guān)視網(wǎng)膜病變的潛在藥物。
[Abstract]:Objective: retinal light injury, mainly related to the accumulation of reactive oxygen species (ROS), is an important mechanism of ARMD,RP and many other retinal diseases. Hydrogen is a selective antioxidant. Hydrogen dissolved in liquid can selectively neutralize hydroxyl radical and peroxynitrite anion which are the most important medium for oxidative damage. In this experiment, we investigated the effects of saturated hydrogen saline solution on retinal blue light injury in rats. Methods: 100 healthy female rats (160g-180g) were randomly divided into four groups. The rats of the four groups were fed in 12 H light 12 H dark environment and could eat freely. During the modeling period, one group was not treated, two and three groups were given 1ml/100g saline and saturated hydrogen saline intraperitoneally according to the weight of the rats, and the four groups were taken as the control group. After 20 days, the rat model of retinal light injury was established after 6 hours of blue light irradiation. Retinal parameters were measured at 8 hours, 16 hours and 24 hours after illumination. After sampling, the degree of retinal injury and the effect of saturated hydrogen saline were observed by H-E staining, ultrastructure observation and biochemical index detection. Results: the results of H-E test showed that the damage of retinal structure was severe in one group and two groups, and light in three groups. There was no significant difference between one group and two groups. Compared with one group and two groups, the retinal structure in the three groups was clear and the cells were arranged neatly. The thickness of the outer nuclear layer was significantly different between each group except one group and two groups (p0.05). At three time points, the thickness of outer nuclear layer was 30.41 鹵4.04 渭 m, 26.11 鹵2.82 渭 m and 20.63 鹵1.06 渭 m in one group, 31.62 鹵4.5 渭 m, 25.08 鹵3.63 渭 m and 19.07 鹵3.86 渭 m in group two, 29.75 鹵3.62 渭 m, 28.83 鹵1.97 渭 m and 27.61 鹵1.813 渭 m in group 3, and 37.35 鹵1.37 渭 mm. in group 4, respectively. From this, it is concluded that as time goes on, the damage becomes more and more serious. At 24 hours, the difference was greatest. Compared with the four groups, one group was 46.23, the second group was 50.29, and the third group was 28.04 thin. Electron microscope showed that the injury in the three groups was significantly lighter than that in the first and second groups. Compared with the four groups, the content of dialdehyde in one group was significantly higher than that in the four groups at each time point (p 0.05). The malondialdehyde content in the three groups was significantly lower than that in the first group (p 0.05) and the second group (p 0.05). There was no significant difference between one group and two groups at each time point (p0.05). Conclusion: the results show that saturated hydrogen saline can effectively protect retina and reduce light damage, which is related to the antioxidant effect of hydrogen. These findings make hydrogen a potential drug in the treatment of oxidative stress-related retinopathy caused by light damage such as ARMD.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2012
【分類號】：R774.1
本文編號：2269330
[Abstract]:Objective: retinal light injury, mainly related to the accumulation of reactive oxygen species (ROS), is an important mechanism of ARMD,RP and many other retinal diseases. Hydrogen is a selective antioxidant. Hydrogen dissolved in liquid can selectively neutralize hydroxyl radical and peroxynitrite anion which are the most important medium for oxidative damage. In this experiment, we investigated the effects of saturated hydrogen saline solution on retinal blue light injury in rats. Methods: 100 healthy female rats (160g-180g) were randomly divided into four groups. The rats of the four groups were fed in 12 H light 12 H dark environment and could eat freely. During the modeling period, one group was not treated, two and three groups were given 1ml/100g saline and saturated hydrogen saline intraperitoneally according to the weight of the rats, and the four groups were taken as the control group. After 20 days, the rat model of retinal light injury was established after 6 hours of blue light irradiation. Retinal parameters were measured at 8 hours, 16 hours and 24 hours after illumination. After sampling, the degree of retinal injury and the effect of saturated hydrogen saline were observed by H-E staining, ultrastructure observation and biochemical index detection. Results: the results of H-E test showed that the damage of retinal structure was severe in one group and two groups, and light in three groups. There was no significant difference between one group and two groups. Compared with one group and two groups, the retinal structure in the three groups was clear and the cells were arranged neatly. The thickness of the outer nuclear layer was significantly different between each group except one group and two groups (p0.05). At three time points, the thickness of outer nuclear layer was 30.41 鹵4.04 渭 m, 26.11 鹵2.82 渭 m and 20.63 鹵1.06 渭 m in one group, 31.62 鹵4.5 渭 m, 25.08 鹵3.63 渭 m and 19.07 鹵3.86 渭 m in group two, 29.75 鹵3.62 渭 m, 28.83 鹵1.97 渭 m and 27.61 鹵1.813 渭 m in group 3, and 37.35 鹵1.37 渭 mm. in group 4, respectively. From this, it is concluded that as time goes on, the damage becomes more and more serious. At 24 hours, the difference was greatest. Compared with the four groups, one group was 46.23, the second group was 50.29, and the third group was 28.04 thin. Electron microscope showed that the injury in the three groups was significantly lighter than that in the first and second groups. Compared with the four groups, the content of dialdehyde in one group was significantly higher than that in the four groups at each time point (p 0.05). The malondialdehyde content in the three groups was significantly lower than that in the first group (p 0.05) and the second group (p 0.05). There was no significant difference between one group and two groups at each time point (p0.05). Conclusion: the results show that saturated hydrogen saline can effectively protect retina and reduce light damage, which is related to the antioxidant effect of hydrogen. These findings make hydrogen a potential drug in the treatment of oxidative stress-related retinopathy caused by light damage such as ARMD.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2012
【分類號】：R774.1
【參考文獻(xiàn)】
相關(guān)期刊論文 前2條
1 周建維,任國良,張曉明,朱f^,林海燕,周吉林;維生素C對視網(wǎng)膜色素上皮藍(lán)光損傷保護(hù)作用的研究(簡報)[J];實驗生物學(xué)報;2003年05期
2 安美霞,張效房,張金嵩;挫傷性視網(wǎng)膜病變中光感受器細(xì)胞凋亡與氧化損傷的實驗研究[J];中華眼科雜志;2004年02期
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