【摘要】：目的:近年來(lái),大量研究證實(shí)阻塞性睡眠呼吸暫停綜合征(obstructive sleep apnea syndrome;OSAS)能夠?qū)е赂喂δ艿膿p害。慢性間歇低氧(chronic intermittent hypoxia;CIH)作為OSAS主要的病理生理學(xué)特征,可誘發(fā)非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)。本研究主要目的是探討 OS AS 模式CIH對(duì)大鼠肝功能的影響及脂聯(lián)素的保護(hù)機(jī)制。方法:60只健康雄性6周齡Wistar大鼠(SPF級(jí)別,體重180-200g)按隨機(jī)數(shù)字表法分為正常對(duì)照組、正常脂聯(lián)素組、CIH組和CIH +脂聯(lián)素組各15只。對(duì)CIH組和CIH+脂聯(lián)素組給予每天8小時(shí)的CIH,持續(xù)處理4個(gè)月。同時(shí)正常對(duì)照組和正常脂聯(lián)素組大鼠接受正常氣壓空氣處理。正常脂聯(lián)素組和CIH+脂聯(lián)素組大鼠接受每周2次脂聯(lián)素尾靜脈注射,每次注射量10μg,為期4個(gè)月。造模完成后比較組間血漿天冬氨酸轉(zhuǎn)氨酶(AST)、丙氨酸轉(zhuǎn)氨酶(ALT)水平以及內(nèi)質(zhì)網(wǎng)應(yīng)激(endoplasmic reticulum stress,ERS)和線粒體相關(guān)的細(xì)胞凋亡狀況。結(jié)果:正常對(duì)照組和正常脂聯(lián)素組各項(xiàng)觀察指標(biāo)間差異均無(wú)統(tǒng)計(jì)學(xué)意義(均P0.05)。CIH 組 AST 和 ALT 水平[(319±21)和(113±9)U/L]均顯著高于正常對(duì)照組[(178±19)和(51±9)U/L)]和正常脂聯(lián)素組[(175±16)和(52±8)U/L](均P0.01)。與正常對(duì)照組和正常脂聯(lián)素組相比,CIH組大鼠肝組織出現(xiàn)較明顯的ERS及線粒體相關(guān)的細(xì)胞凋亡改變。而上述改變?cè)贑IH +脂聯(lián)素組均較CIH組顯著減輕(均P0.05)。結(jié)論:CIH可以引起大鼠肝功能損傷,脂聯(lián)素可能通過(guò)抑制ERS及線粒體相關(guān)凋亡通路而起到保護(hù)作用。
[Abstract]:Objective: in recent years, a large number of studies have proved that obstructive sleep apnea syndrome (obstructive sleep apnea syndrome;OSAS) can lead to liver function damage. Chronic intermittent hypoxia (chronic intermittent hypoxia;CIH), as the main pathophysiological feature of OSAS, can induce non-alcoholic fatty liver disease (non-alcoholic fatty liver disease,NAFLD). The aim of this study was to investigate the effects of OS AS CIH on liver function and the protective mechanism of adiponectin in rats. Methods Sixty healthy male 6-week-old Wistar rats (SPF grade, body weight 180-200g) were randomly divided into normal control group, normal adiponectin group (n = 15) and CIH adiponectin group (n = 15). CIH group and CIH adiponectin group were treated with 8 hours CIH, for 4 months. At the same time, normal control group and normal adiponectin group were treated with normal air pressure. Rats in normal adiponectin group and CIH adiponectin group received adiponectin caudal injection twice a week for 4 months. The plasma aspartate aminotransferase (AST),) alanine aminotransferase (ALT) levels, endoplasmic reticulum stress (endoplasmic reticulum stress,ERS) and mitochondrial apoptosis were compared. Results: there was no significant difference between normal adiponectin group and normal adiponectin group (P0.05). The levels of AST and ALT in CIH group [(319 鹵21) and (113 鹵9) U / L] were significantly higher than those in normal control group [(178 鹵19) and (51 鹵9) U / L] and normal adiponectin group [(175 鹵16) and (52 鹵8) U / L] (P0.01). Compared with the normal control group and the normal adiponectin group, there were obvious changes of ERS and mitochondria related apoptosis in the liver tissue of the rats. These changes were significantly reduced in CIH adiponectin group than in CIH group (P0.05). ConclusionCIH can induce liver function injury in rats. Adiponectin may play a protective role by inhibiting ERS and mitochondria related apoptosis pathway.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R766

【參考文獻(xiàn)】

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