發(fā)布時間：2018-09-14 10:10

【摘要】：目的 益生菌DNA滴鼻干預(yù)對卵清蛋白(OVA)誘發(fā)的變應(yīng)性鼻炎療效的動物模型研究，探討小鼠鼻黏膜中調(diào)節(jié)性T細(xì)胞（Treg細(xì)胞）及血清Th1/ Th2細(xì)胞因子水平。 方法 培養(yǎng)益生菌，提取DNA，取6-8周Balb/c小鼠隨機(jī)分為益生菌DNA滴鼻組及生理鹽水對照組，每組15只。益生菌滴鼻組：30％的卵清蛋白（OVA）生理鹽水溶液，每毫升加入AL(OH)330 mg腹腔注射大鼠，隔日1次，每次1 ml，7次后用5％的OVA液滴鼻，每側(cè)l0ul，每天1次，共7次，致敏的同時取已提取的DNA液滴鼻，每側(cè)5ul（25ug/ml）,每天一次。對照組：OVA造模方法同益生菌滴鼻組，但以生理鹽水代替益生菌DNA液滴鼻。采用ELISA法檢測各組血清IgE及IL-4，IL-10，IFN-γ，IFN-γ/ IL-4水平。分析鼻黏膜中Foxp3陽性細(xì)胞數(shù)，并對鼻黏膜行組織形態(tài)學(xué)觀察。組間用t檢驗。 結(jié)果 1.益生菌DNA滴鼻組IgE顯著低于對照組（p0.05）， 2.益生菌DNA滴鼻組IL-10，IFN-γ，IL-4均低于對照組（p0.05），而IFN-γ/IL-4高于對照組（p0.05）。 3.益生菌DNA滴鼻組鼻粘膜中Foxp-3陽性細(xì)胞數(shù)顯著高于對照組（p0.05）。 4.益生菌DNA滴鼻組鼻粘膜較對照組出現(xiàn)明顯的形態(tài)學(xué)改變。 結(jié)論 1.益生菌DNA滴鼻干預(yù)通過降低動物模型IgE水平，緩解Ⅰ型變態(tài)反應(yīng)性。 2.益生菌DNA滴鼻通過調(diào)整模型動物Th1/Th2細(xì)胞因子平衡，，抑制Th2細(xì)胞反應(yīng)，對變應(yīng)性鼻炎起一定防治作用。 3.益生菌DNA滴鼻早期干預(yù)可提高動物模型鼻粘膜Foxp3陽性細(xì)胞數(shù)抑制小鼠AR的進(jìn)展。
[Abstract]:Objective to investigate the effects of probiotic DNA nasal drip on allergic rhinitis induced by ovalbumin (OVA) and to investigate the levels of regulatory T cells (Treg cells) and serum Th1/ Th2 cytokines in mouse nasal mucosa. Methods probiotics were cultured and Balb/c mice were randomly divided into probiotic DNA nasal dropping group and normal saline control group for 6 to 8 weeks with 15 mice in each group. Probiotic nasal drip group: 30% ovalbumin (OVA) saline solution was injected intraperitoneally into rats with AL (OH) 330 mg per milliliter. Once a second day, 1 ml,7 per day, 5% OVA solution was used on each side of the nose, 10% per side, once a day, for 7 times. At the same time, the extracted DNA solution was nasal drops, 5ul (25ug/ml) on each side, once a day. The control group was treated with probiotic nasal drip, but normal saline was used instead of probiotic DNA solution. Serum IgE and IL-4,IL-10,IFN- 緯 / IL-4 levels were detected by ELISA assay. The number of Foxp3 positive cells in nasal mucosa was analyzed and the histomorphology of nasal mucosa was observed. T test was used between groups. Result 1. The IgE of probiotic DNA nasal drip group was significantly lower than that of control group (p0.05). The levels of IL-10,IFN- 緯 -IL-4 in probiotic DNA nasal drip group were lower than those in control group (p0.05), while IFN- 緯 -IL-4 was higher than that in control group (p0.05). The number of Foxp-3 positive cells in nasal mucosa of probiotic DNA dropping group was significantly higher than that of control group (p0.05). The nasal mucosa of the probiotic DNA nasal drip group showed obvious morphologic changes compared with the control group. Conclusion 1. Probiotic DNA nasal drip alleviates type 鈪

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