天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

PI3K-AKT信號(hào)通路在PDGF促進(jìn)人晶狀體上皮細(xì)胞增殖中的作用

發(fā)布時(shí)間:2018-08-03 21:46
【摘要】:目的:后發(fā)性白內(nèi)障(posterior capsule opacification,PCO)也稱后囊膜混濁,是白內(nèi)障術(shù)后及晶狀體外傷后最常見(jiàn)的并發(fā)癥。研究表明殘留的晶狀體上皮細(xì)胞增殖在PCO的發(fā)病機(jī)制中發(fā)揮著十分重要的作用,但其增殖的具體原理尚未完全闡明,本研究探討體外應(yīng)用血小板源性生長(zhǎng)因子(platelet derived growth factor,PDGF)及PI3K(phosphatidylinositols-kinase)信號(hào)通路抑制劑LY294002分別或同時(shí)刺激人源晶狀體上皮細(xì)胞(lens epithelial cells,LECs)株SRA01/04,以探討PI3K-AKT信號(hào)通路在PDGF促進(jìn)人晶狀體上皮細(xì)胞增殖中的作用。方法:人晶狀體上皮細(xì)胞株SRA01/04常規(guī)傳代培養(yǎng),用不同濃度的PDGF(0ng/ml,0.1ng/ml,1ng/ml,10ng/ml,100ng/ml),不同濃度LY294002(0μM,5μM,10μM,20μM,40μM)以及不同濃度的PDGF(10ng/ml)+LY294002(0μM,5μM,10μM,20μM,40μM)分別作用于SRA01/04細(xì)胞24小時(shí),MTT法檢測(cè)各組細(xì)胞的增殖率或增殖抑制率;根據(jù)MTT結(jié)果,選定各藥物組濃度,將實(shí)驗(yàn)分為四組,即空白對(duì)照組、PDGF組(PDGF濃度為10ng/ml)、LY294002組(LY294002濃度為40μM)、PDGF+LY294002組(10ng/ml的PDGF+40μM的LY294002),各組細(xì)胞培養(yǎng)24h,倒置顯微鏡下觀察各組晶狀體上皮細(xì)胞增殖、抑制的情況;蛋白印跡法檢測(cè)各組細(xì)胞中總AKT及P-AKT的蛋白表達(dá)量差異。結(jié)果:1、人晶狀體上皮細(xì)胞SRA01/04的增殖率與PDGF的濃度呈正相關(guān)(P0.001);2、人晶狀體上皮細(xì)胞SRA01/04的增殖的抑制率與LY294002的濃度呈正相關(guān)(P0.001);3、PDGF促人晶狀體上皮細(xì)胞SRA01/04的增殖作用與LY294002的濃度呈負(fù)相關(guān)(P0.001);4、PDGF、LY294002分別或同時(shí)干預(yù)人晶狀體上皮細(xì)胞SRA01/04時(shí)細(xì)胞總AKT表達(dá)無(wú)明顯差異(P=0.066);5、PDGF單獨(dú)干預(yù)人晶狀體上皮細(xì)胞SRA01/04時(shí)細(xì)胞p-AKT表達(dá)量增加,差異有統(tǒng)計(jì)學(xué)意義(P0.001);6、LY294002單獨(dú)干預(yù)人晶狀體上皮細(xì)胞SRA01/04時(shí)細(xì)胞p-AKT表達(dá)量減少,差異有統(tǒng)計(jì)學(xué)意義(P0.001);7、PDGF和LY294002同時(shí)干預(yù)人晶狀體上皮細(xì)胞SRA01/04時(shí)細(xì)胞p-AKT表達(dá)量較PDGF單獨(dú)干預(yù)時(shí)減少而較LY294002單獨(dú)干預(yù)時(shí)增加,差異有統(tǒng)計(jì)學(xué)意義(P0.001)。結(jié)論:1、PDGF能促進(jìn)人晶狀體上皮細(xì)胞的增殖;2、LY294002能抑制人晶狀體上皮細(xì)胞的增殖;3、PDGF促進(jìn)人晶狀體上皮細(xì)胞的增殖作用能被LY294002抑制;4、PDGF通過(guò)激活PI3K/AKT信號(hào)通路促進(jìn)人晶狀體上皮細(xì)胞的增殖。
[Abstract]:Objective: posterior capsular opacification (posterior capsule) is the most common complication after cataract surgery and lens trauma. Studies have shown that the residual lens epithelial cell proliferation plays a very important role in the pathogenesis of PCO, but the specific mechanism of its proliferation has not been fully clarified. In this study, we used platelet-derived growth factor (platelet derived growth factor-PDGF (PDGF) and PI3K (phosphatidylinositols-kinase) signal pathway inhibitor LY294002 to stimulate human lens epithelial cell line SRA01 / 04, respectively or simultaneously, in order to explore the effect of PI3K-AKT signaling pathway on the promotion of PDGF on human lens. The role of skin cell proliferation. Methods: human lens epithelial cell line SRA01/04 was subcultured routinely. SRA01/04 cells were treated with different concentrations of PDGF (0 ng / ml 0.1 ng / ml 10 ng / ml 10ng / ml), different concentrations of LY294002 (0 渭 M 5 渭 M 10 渭 M ~ 10 渭 M) and different concentrations of PDGF (10ng/ml) LY294002 (0 渭 M 5 渭 M 5 渭 M 10 渭 M 10 渭 M 10 渭 M 40 渭 M) for 24 hours respectively to detect the proliferation rate or inhibition rate of SRA01/04 cells. Each drug group was selected and divided into four groups: the blank control group (10ng/ml) (LY294002 40 渭 M) and PDGF LY294002 (40 渭 M). The cells were cultured for 24 hours. The proliferation and inhibition of lens epithelial cells in each group were observed under inverted microscope. The protein expression of total AKT and P-AKT was detected by Western blot. Results the proliferation rate of SRA01/04 in human lens epithelial cells was positively correlated with the concentration of PDGF (P0.001) and the inhibitory rate of SRA01/04 proliferation in human lens epithelial cells was positively correlated with the concentration of LY294002 (P0.001). There was no significant difference (P0. 066) in the total AKT expression of human lens epithelial cells (P0. 066). P0. 066 (P0. 066) P0. 05 PDGF alone interfered with the increase of p-AKT expression in SRA01/ 04:00 cells of human lens epithelial cells. The difference was statistically significant (P0.001). LY294002 alone interfered with the decrease of p-AKT expression in SRA01/ 04:00 cells of human lens epithelial cells. The difference was statistically significant (P0. 001). The p-AKT expression of SRA01/ 04:00 cells in human lens epithelial cells treated with P0. 001 PDGF and LY294002 at the same time was lower than that with PDGF alone and increased compared with LY294002 alone. The difference was statistically significant (P0. 001). Conclusion LY294002 can inhibit the proliferation of human lens epithelial cells by activating PI3K/AKT signaling pathway.
【學(xué)位授予單位】:桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2017
【分類號(hào)】:R776.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張春梅;劉紅玲;;PI3K-AKT-mTOR信號(hào)通路在后發(fā)性白內(nèi)障中作用的研究進(jìn)展[J];中華實(shí)用診斷與治療雜志;2017年03期

2 杜文文;張鳳妍;;后發(fā)性白內(nèi)障與相關(guān)信號(hào)通路的研究進(jìn)展[J];國(guó)際眼科縱覽;2016年03期

3 郭彬;周艷峰;;蕓香苷通過(guò)PI3K/AKT信號(hào)通路抑制H_2O_2誘導(dǎo)人晶狀體上皮細(xì)胞凋亡[J];安徽醫(yī)科大學(xué)學(xué)報(bào);2015年08期

4 陳瑤;艾明;;苦參堿對(duì)視網(wǎng)膜母細(xì)胞瘤細(xì)胞增殖、凋亡及PI3K/Akt信號(hào)通路的影響[J];臨床腫瘤學(xué)雜志;2015年07期

5 趙寧;張瑞君;鐘一凡;劉磊;李佳;;環(huán)孢素A對(duì)兔晶狀體上皮細(xì)胞增殖過(guò)程中磷酸肌醇-3激酶途徑的影響[J];國(guó)際眼科雜志;2014年12期

6 諶金金;王平;;藥物防治后發(fā)性白內(nèi)障的研究進(jìn)展[J];海南醫(yī)學(xué);2014年22期

7 宋慶磊;張海江;霍鳴;;生長(zhǎng)因子/信號(hào)轉(zhuǎn)導(dǎo)通路與后發(fā)性白內(nèi)障[J];廣東醫(yī)學(xué);2014年08期

8 趙寧;張瑞君;劉磊;李佳;劉寧寧;;PTEN基因?qū)ν镁铙w上皮細(xì)胞增殖抑制作用的實(shí)驗(yàn)研究[J];眼科新進(jìn)展;2014年01期

9 郭瑞;郭?;孟倩麗;;LY294002對(duì)人晶狀體上皮細(xì)胞蛋白激酶B活化的影響[J];中華實(shí)驗(yàn)眼科雜志;2013年11期

10 李華;湯欣;;后發(fā)性白內(nèi)障與相關(guān)信號(hào)轉(zhuǎn)導(dǎo)通路研究進(jìn)展[J];中國(guó)實(shí)用眼科雜志;2013年11期



本文編號(hào):2163087

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/wuguanyixuelunwen/2163087.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶b441c***提供,本站僅收錄摘要或目錄,作者需要?jiǎng)h除請(qǐng)E-mail郵箱bigeng88@qq.com
中文字幕久久精品亚洲乱码| 国产成人av在线免播放观看av | 国产美女精品午夜福利视频| 日韩免费国产91在线| 黄色片一区二区在线观看| 国产精品自拍杆香蕉视频| 国产精品人妻熟女毛片av久| 国产精品美女午夜视频| 欧美成人精品国产成人综合| 久七久精品视频黄色的| 亚洲高清欧美中文字幕| 日本熟妇五十一区二区三区| 一本色道久久综合狠狠躁| 欧美一区二区不卡专区| 九九热这里只有精品哦| 久久三级国外久久久三级| 99精品国产自在现线观看| 91免费一区二区三区| 91熟女大屁股偷偷对白| 国产一级特黄在线观看| 日韩黄片大全免费在线看 | 国产美女精品午夜福利视频| 男人的天堂的视频东京热| 好吊一区二区三区在线看| 久草国产精品一区二区| 国产精品制服丝袜美腿丝袜| 成年男女午夜久久久精品| 精品偷拍一区二区三区| 日韩少妇人妻中文字幕| 亚洲精品一区二区三区免 | 亚洲欧美国产精品一区二区| 亚洲成人免费天堂诱惑| 欧美大黄片在线免费观看| 好吊日成人免费视频公开| 日本东京热视频一区二区三区| 亚洲超碰成人天堂涩涩| 亚洲中文字幕人妻系列| 欧美精品激情视频一区| 亚洲欧美日韩国产成人| 日韩一区二区三区嘿嘿| 人妻一区二区三区多毛女|