PI3K-AKT信號(hào)通路在PDGF促進(jìn)人晶狀體上皮細(xì)胞增殖中的作用
[Abstract]:Objective: posterior capsular opacification (posterior capsule) is the most common complication after cataract surgery and lens trauma. Studies have shown that the residual lens epithelial cell proliferation plays a very important role in the pathogenesis of PCO, but the specific mechanism of its proliferation has not been fully clarified. In this study, we used platelet-derived growth factor (platelet derived growth factor-PDGF (PDGF) and PI3K (phosphatidylinositols-kinase) signal pathway inhibitor LY294002 to stimulate human lens epithelial cell line SRA01 / 04, respectively or simultaneously, in order to explore the effect of PI3K-AKT signaling pathway on the promotion of PDGF on human lens. The role of skin cell proliferation. Methods: human lens epithelial cell line SRA01/04 was subcultured routinely. SRA01/04 cells were treated with different concentrations of PDGF (0 ng / ml 0.1 ng / ml 10 ng / ml 10ng / ml), different concentrations of LY294002 (0 渭 M 5 渭 M 10 渭 M ~ 10 渭 M) and different concentrations of PDGF (10ng/ml) LY294002 (0 渭 M 5 渭 M 5 渭 M 10 渭 M 10 渭 M 10 渭 M 40 渭 M) for 24 hours respectively to detect the proliferation rate or inhibition rate of SRA01/04 cells. Each drug group was selected and divided into four groups: the blank control group (10ng/ml) (LY294002 40 渭 M) and PDGF LY294002 (40 渭 M). The cells were cultured for 24 hours. The proliferation and inhibition of lens epithelial cells in each group were observed under inverted microscope. The protein expression of total AKT and P-AKT was detected by Western blot. Results the proliferation rate of SRA01/04 in human lens epithelial cells was positively correlated with the concentration of PDGF (P0.001) and the inhibitory rate of SRA01/04 proliferation in human lens epithelial cells was positively correlated with the concentration of LY294002 (P0.001). There was no significant difference (P0. 066) in the total AKT expression of human lens epithelial cells (P0. 066). P0. 066 (P0. 066) P0. 05 PDGF alone interfered with the increase of p-AKT expression in SRA01/ 04:00 cells of human lens epithelial cells. The difference was statistically significant (P0.001). LY294002 alone interfered with the decrease of p-AKT expression in SRA01/ 04:00 cells of human lens epithelial cells. The difference was statistically significant (P0. 001). The p-AKT expression of SRA01/ 04:00 cells in human lens epithelial cells treated with P0. 001 PDGF and LY294002 at the same time was lower than that with PDGF alone and increased compared with LY294002 alone. The difference was statistically significant (P0. 001). Conclusion LY294002 can inhibit the proliferation of human lens epithelial cells by activating PI3K/AKT signaling pathway.
【學(xué)位授予單位】:桂林醫(yī)學(xué)院
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2017
【分類號(hào)】:R776.1
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