【摘要】：目的 探討硫酸鋅（ZnSO_4）對紫外線誘導的大鼠晶狀體氧化損傷的保護作用。 方法 培養(yǎng)離體大鼠晶狀體，，將其隨機分為三組：（1）陰性對照組（正常組）；（2）陽性對照組（紫外線照射組）；（3）實驗組(紫外線照射+ZnSO_4組)。觀察晶狀體的混濁程度，取各組晶狀體標本進行蘇木素/伊紅染色（HE）染色，光鏡下觀察組織形態(tài)學變化，檢測晶狀體組織中谷胱甘肽過氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）及晶狀體中熱休克蛋白70（HSP70）表達的變化。 結果 陰性對照組各時段的晶狀體基本保持透明；陽性對照組晶狀體隨著培養(yǎng)時間的延長逐漸混濁；實驗組晶狀體隨著培養(yǎng)時間的延長逐漸混濁，但與同時段陽性對照組相比，混濁程度減輕。光學顯微鏡下見陰性對照組晶狀體組織結構基本正常；陽性對照組與實驗組晶狀體組織結構有不同程度的損傷；實驗組晶狀體損傷程度較同時段陽性對照組減輕。陽性對照組晶狀體中GSH-Px和SOD活性低于同時段陰性對照組，差異有統(tǒng)計學意義（P0.05）；實驗組晶狀體中GSH-Px和SOD活性高于同時段陽性對照組，差異有統(tǒng)計學意義（P0.05）。陽性對照組晶狀體中HSP70的表達與同時段陰性對照組相比，表達增加，差異有統(tǒng)計學意義（P0.05）；實驗組晶狀體中HSP70的表達與同時段陽性對照組相比，表達增加，差異有統(tǒng)計學意義（P0.05）。 結論 ZnSO_4可以誘導離體大鼠晶狀體中HSP70的表達，可以提高氧化損傷晶狀體的抗氧化能力，在維持晶狀體透明性方面起到了一定的作用。
[Abstract]:Objective to investigate the protective effect of zinc sulfate (ZnSO _ 4) on ultraviolet induced oxidative injury of rat lens. Methods isolated rat lens were cultured and randomly divided into three groups: (1) negative control group (normal group,); (_ 2), positive control group (ultraviolet radiation group,); (_ 3), experimental group (ultraviolet radiation); (_ 3 group). The degree of opacity of lens was observed. Hematoxylin / eosin (HE) staining and histomorphology were observed under light microscope. The changes of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and heat shock protein 70 (HSP70) in lens were detected. Results the lens of the negative control group was basically transparent at every time, the lens of the positive control group was gradually opacified with the extension of culture time, and the lens of the experimental group was gradually opacified with the extension of the culture time. But compared with the positive control group at the same time, the degree of turbidity decreased. Under the optical microscope, the structure of the lens in the negative control group was basically normal, that in the positive control group and the experimental group was different degrees, and the degree of lens injury in the experimental group was less than that in the positive control group in the same period. The activities of GSH-Px and SOD in lens of positive control group were lower than those of negative control group in the same period (P0.05); the activities of GSH-Px and SOD in lens of experimental group were higher than that of positive control group in the same period (P0.05). The expression of HSP70 in the lens of the positive control group was higher than that of the negative control group in the same period (P0.05), while the expression of HSP70 in the lens of the experimental group was higher than that of the positive control group in the same period (P0.05). Conclusion ZnSOS _ 4 can induce the expression of HSP70 in isolated rat lens, increase the anti-oxidation ability of the injured lens, and play a certain role in maintaining the transparency of the lens.
【學位授予單位】：遼寧醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R77

【參考文獻】

相關期刊論文 前10條

1 李義杰;張保國;;熱休克蛋白的生物學功能及其對細胞的輻射防護作用[J];輻射防護通訊;2007年06期

2 任寶波,王玉艷,王純凈,李士澤,楊煥民;HSP70家族的分類及基因結構與功能[J];動物醫(yī)學進展;2005年01期

3 仝佳;王勝軍;肖壽華;;熱休克蛋白及其在眼部疾病中作用的研究進展[J];國際眼科雜志;2006年01期

4 周星利;亢澤峰;李凌;馬雪英;張蓉;;熱休克蛋白與白內(nèi)障發(fā)生的研究進展[J];國際眼科雜志;2008年04期

5 劉瑜;魯建華;張文芳;李姍姍;曲來強;;谷氨酰胺體外對大鼠晶狀體上皮細胞HSP70和NF-κB表達的影響[J];國際眼科雜志;2010年09期

6 饒惠英,姚克;熱休克蛋白在眼晶狀體的表達與熱耐受[J];國外醫(yī)學(眼科學分冊);2004年03期

7 宮智勇;牛丕業(yè);譚皓;王峰;鄒云鋒;楊瑾;段燕英;袁晶;鄔堂春;;槲皮素抑制HSP70表達的體外研究[J];工業(yè)衛(wèi)生與職業(yè)病;2007年02期

8 李瑩;王玉清;曹麗輝;馮瀾;;熱休克蛋白70在老年性白內(nèi)障晶狀體上皮細胞中的表達及意義[J];黑龍江醫(yī)藥科學;2006年04期

9 楊震國;;熱休克蛋白70的生物學功能及應用的研究進展[J];中國飼料;2010年23期

10 周云;微量元素與眼病[J];微量元素與健康研究;2004年05期

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