本文選題：表沒食子兒茶素沒食子酸酯 + 豚鼠�。� 參考：《聽力學(xué)及言語疾病雜志》2017年03期

【摘要】：目的探討表沒食子兒茶素沒食子酸酯(epigallocatechin-3-gallate,EGCG)對噪聲性耳蝸損傷的影響。方法 45只豚鼠隨機(jī)分為EGCG+噪聲組、生理鹽水+噪聲組、正常對照組,每組15只。EGCG+噪聲組和生理鹽水+噪聲組豚鼠在接受噪聲暴露(120dB SPL,4h)前一日及每次暴露前1h分別腹腔注射EGCG(25mg/1 000g)和等量生理鹽水,正常對照組不予任何處理。噪聲暴露后即刻和第1、3、7、14天檢測三組豚鼠聽性腦干反應(yīng)(ABR),第14天分離各組豚鼠耳蝸,行耳蝸基底膜、血管紋鋪片及免疫組化染色,觀察各組豚鼠耳蝸基底膜、血管紋細(xì)胞形態(tài)及外毛細(xì)胞運(yùn)動蛋白(Prestin)、3-硝基酪氨酸(3-NT)分布的變化。結(jié)果噪聲暴露后,EGCG+噪聲組各時間點(diǎn)的ABR反應(yīng)閾均高于對照組,但低于生理鹽水+噪聲組,從第3天開始,與兩組間ABR反應(yīng)閾差異縮小,但差異仍有統(tǒng)計學(xué)意義(均為P0.05)。免疫組織化學(xué)染色顯示,正常對照組Prestin蛋白顯綠染的耳蝸三排外毛細(xì)胞排列整齊,無細(xì)胞缺失,3-NT主要分布于毛細(xì)胞胞質(zhì)及表皮板;與生理鹽水+噪聲組相比,噪聲暴露后,EGCG+噪聲組豚鼠外毛細(xì)胞形態(tài)較好,Prestin染色清晰;基底膜、血管紋處損傷輕,細(xì)胞排列規(guī)則,3-NT分布減少。結(jié)論預(yù)防性腹腔注射EGCG可減輕噪聲引起的耳蝸損傷,對噪聲性聽力損傷有一定的防護(hù)作用。
[Abstract]:Objective to investigate the effect of epigallocatechin-3-gallate EGCG on noise-induced cochlear injury. Methods Forty-five guinea pigs were randomly divided into EGCG noise group, normal saline noise group and normal control group. 15 guinea pigs in each group were given intraperitoneal injection of EGCG (25mg/1 000g) and the same amount of normal saline (25mg/1 000g) one day before and one hour before each exposure to noise exposure (120dB SPLA), while the normal control group did not receive any treatment. Auditory brainstem response (ABR) was detected immediately after noise exposure and 714 days after noise exposure. Cochlea were isolated from each group on the 14th day, and cochlear basement membrane, stria vascularis and immunohistochemical staining were performed to observe the basal membrane of cochlea in each group. Morphological changes of stria vascularis cells and distribution of 3-nitrotyrosine (3-NT) in outer hair cell motility protein (Prestin). Results the ABR response threshold of EGCG noise group was higher than that of control group, but lower than that of normal saline noise group. From the 3rd day, the difference of ABR reaction threshold between EGCG noise group and two groups was reduced, but the difference was still statistically significant (P0.05). Immunohistochemical staining showed that the outer hair cells of the cochlea with prestin protein green staining in the normal control group were arranged neatly, and the 3-NT was mainly distributed in the cytoplasm and epidermis of the hair cells, compared with the normal saline noise group. After noise exposure, the outer hair cells of guinea pigs in EGCG noise group were clearly stained with prestin, the basal membrane and stria vascularis were slightly damaged, and the regular distribution of cells was decreased. Conclusion prophylactic intraperitoneal injection of EGCG can attenuate the cochlear injury induced by noise and has a protective effect on noise-induced hearing impairment.
【作者單位】： 中國人民解放軍總醫(yī)院耳鼻咽喉頭頸外科;中國人民解放軍第163醫(yī)院耳鼻咽喉頭頸外科;
【基金】：國家自然科學(xué)基金面上項目(81170908,81470683)資助
【分類號】：R764

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