發(fā)布時(shí)間：2018-07-04 22:35

  本文選題：芪菊袋泡茶 + EB病毒感染　； 參考：《廣州中醫(yī)藥大學(xué)》2012年碩士論文

【摘要】：背景 EB病毒是一種具有復(fù)雜基因組的人類(lèi)皰疹病毒,全球90%以上的人受到過(guò)該病毒的感染。EB病毒對(duì)人類(lèi)具有普遍易感性且與多種人類(lèi)疾病尤其是惡性腫瘤疾病密切相關(guān)。EB病毒在人體內(nèi)感染情況對(duì)于鼻咽癌(NPC)的診斷、臨床分期、預(yù)后判斷和監(jiān)測(cè)放療后轉(zhuǎn)移復(fù)發(fā)等均具有重要意義。有研究者提出EB病毒是鼻咽癌的主要致病因素之一。EB病毒與鼻咽炎也有密切的相關(guān)性,研究發(fā)現(xiàn)EB病毒感染者較EB病毒陰性者有較高的鼻咽炎患病率。 研究目的 本研究通過(guò)免疫試驗(yàn),客觀評(píng)價(jià)芪菊袋泡茶藥效學(xué)上增強(qiáng)小鼠非特異性免疫功能。通過(guò)對(duì)EB病毒感染的臨床療效觀察,與對(duì)照組即未使用任何臨床干預(yù)組作對(duì)比,觀察芪菊袋泡茶對(duì)EB病毒感染的治療效果,以期為中醫(yī)藥治療EB病毒感染提供一種療效肯定又確切可行的治療手段EB病毒,對(duì)EB病毒感染的預(yù)防和治療及提高中醫(yī)藥治療在EB病毒感染治療中的地位均具有重要意義。 研究方法 1.藥效學(xué)實(shí)驗(yàn)：將小鼠稱(chēng)重、編號(hào)后,隨機(jī)分為實(shí)驗(yàn)組,標(biāo)準(zhǔn)對(duì)照組及空白對(duì)照組,其中實(shí)驗(yàn)組分為高劑量組,中劑量組,低劑量組。按實(shí)驗(yàn)方案灌胃給藥,注射雞紅細(xì)胞懸液,處死小鼠,抽取腹腔液0.5ml,滴于干凈的載玻片上,每片0.5ml,每只小白鼠2片。然后放在墊有濕紗布的搪瓷盒中,37℃孵育半小時(shí)。取出玻片自來(lái)水沖去貼壁的細(xì)胞,自然涼干。用丙酮-甲醇溶液(1：1)固定5分鐘后取出。Giemsa-W right混合染色3～5分鐘,自來(lái)水沖洗,晾干后,油鏡觀查計(jì)數(shù)200個(gè)巨噬細(xì)胞,計(jì)算吞噬指數(shù)(吞噬指數(shù)=被吞噬的雞紅細(xì)胞總數(shù)/200個(gè)巨噬細(xì)胞)和吞噬百分率(吞噬百分率=吞噬雞紅細(xì)胞的巨噬細(xì)胞數(shù)/200個(gè)巨噬細(xì)胞)。記錄整理全部數(shù)據(jù),用方差分析進(jìn)行統(tǒng)計(jì)分析。 2.臨床療效觀察：制定病例納入標(biāo)準(zhǔn)和排除標(biāo)準(zhǔn),將EB病毒感染或EB病毒感染合并氣虛熱毒鼻咽炎的患者采用查隨機(jī)數(shù)字表后隨機(jī)分配成兩組,一組為芪菊袋泡茶治療組,口服袋泡茶,一次2包,開(kāi)水浸泡15分鐘后飲服,一天2次；一組為空白對(duì)照組,即未進(jìn)行任何臨床干預(yù)組。分別在3周后復(fù)查EB病毒,觀察EB病毒轉(zhuǎn)陰情況。 3.統(tǒng)計(jì)分析：計(jì)數(shù)資料采用卡方檢驗(yàn),計(jì)量資料采用t檢驗(yàn),全部數(shù)據(jù)均輸入計(jì)算機(jī)數(shù)據(jù)庫(kù),應(yīng)用SPSS13.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)分析,取檢驗(yàn)水準(zhǔn)為a=0.05。分別對(duì)兩組患者前后的EB病毒轉(zhuǎn)陰率進(jìn)行對(duì)比,組間對(duì)比,比較兩組間療效。 研究結(jié)果 1.藥效學(xué)實(shí)驗(yàn) 統(tǒng)計(jì)吞噬率(吞噬百分率=吞噬雞紅細(xì)胞的巨噬細(xì)胞數(shù)/200個(gè)巨噬細(xì)胞)及吞噬指數(shù)(吞噬指數(shù)=被吞噬的雞紅細(xì)胞總數(shù)/200個(gè)巨噬細(xì)胞),5組間通過(guò)方差分析,方差齊性檢驗(yàn)P0.05,方差齊性,各組間有可比性。組間比較,P0.05,實(shí)驗(yàn)組、標(biāo)準(zhǔn)對(duì)照組與空白對(duì)照組之間吞噬率及吞噬指數(shù)的對(duì)比均有差別,實(shí)驗(yàn)組與標(biāo)準(zhǔn)對(duì)照組及空白對(duì)照組對(duì)比具有差異,中劑量組與高劑量組、低劑量組、標(biāo)準(zhǔn)對(duì)照組及空白對(duì)照組之間均有差異,具有統(tǒng)計(jì)學(xué)意義,表明芪菊袋泡茶有增強(qiáng)小鼠非特異性免疫功能。 2.臨床療效觀察 所有病例來(lái)源于2011年3月至2012年4月廣州中醫(yī)藥大學(xué)第一附屬醫(yī)院耳鼻喉科門(mén)診,共56例,隨機(jī)分為兩組,其中芪菊袋泡茶治療組26例,18例合并有鼻咽炎相關(guān)癥狀,8例無(wú)合并癥狀。空白對(duì)照組30例,合并有鼻咽炎相關(guān)癥狀者為22例,無(wú)合并癥狀者為8例。按要求服藥后隨訪復(fù)查。經(jīng)統(tǒng)計(jì)學(xué)方法統(tǒng)計(jì)分析,對(duì)照組與治療組在治療前總積分、性別,年齡,煙酒嗜好等方面無(wú)差異,P0.05,兩組間基本信息平衡,具有可比性。本次研究中治療組26例患者服用芪菊袋泡茶期間未見(jiàn)明顯不良反應(yīng),3周后復(fù)查血清EB病毒,其中有12例轉(zhuǎn)陰,轉(zhuǎn)陰率為46.2%,對(duì)照組復(fù)查血清EB病毒轉(zhuǎn)陰的有5例,轉(zhuǎn)陰率為16.7%,經(jīng)卡方檢驗(yàn),比較兩組間EB病毒感染轉(zhuǎn)陰率,P0.05,具有統(tǒng)計(jì)學(xué)意義。治療組18例合并有鼻咽炎相關(guān)癥狀者復(fù)查血清EB病毒9例轉(zhuǎn)陰,轉(zhuǎn)陰率為50%。8例無(wú)合并癥狀者轉(zhuǎn)陰的有3例,轉(zhuǎn)陰率為37.5%。對(duì)照組中合并有鼻咽炎相關(guān)癥狀的22例中3例轉(zhuǎn)陰,轉(zhuǎn)陰率為16.7%。8例無(wú)合并癥狀者轉(zhuǎn)陰的有2例,轉(zhuǎn)陰率為25.0%。經(jīng)卡方檢驗(yàn),比較治療組及對(duì)照組有癥狀及無(wú)癥狀者轉(zhuǎn)陰情況,P0.05,無(wú)統(tǒng)計(jì)學(xué)意義。經(jīng)t檢驗(yàn),兩組間治療后總積分差值、主癥差值、次癥差值及體征差值比較,均P0.01,具有顯著性差異。 研究結(jié)論 芪菊袋泡茶在藥效上有增強(qiáng)小鼠非特異性免疫功能的作用,能促進(jìn)EB病毒轉(zhuǎn)陰,并能改善EB病毒感染患者的臨床癥狀。芪菊袋泡茶為純中藥制劑,安全性高,服藥方便且易攜帶,可廣泛推廣于臨床。
[Abstract]:background
EB virus is a human herpesvirus with a complex genome. More than 90% of the world has been infected with the virus, and the.EB virus has universal susceptibility to human beings and is closely related to a variety of human diseases, especially malignant tumor diseases. The diagnosis of nasopharyngeal carcinoma (NPC), clinical stage and prognosis of.EB virus infection in human body (NPC) Some researchers suggest that EB virus is one of the main pathogenic factors of nasopharyngeal carcinoma,.EB virus is also closely related to nasopharyngitis. The study found that EB virus infected people have higher incidence of nasopharyngitis than those with EB virus negative.
research objective
In this study, the non specific immune function of the mice was objectively evaluated through the immunological test. Through the observation of the clinical efficacy of EB virus infection and compared with the control group, that is, the treatment effect of Qi chrysanthemum tea bag tea to EB virus infection was observed in order to provide the treatment of EB virus infection by traditional Chinese medicine. It is of great significance for the prevention and treatment of EB virus infection and the improvement of the status of the treatment of Chinese medicine in the treatment of EB virus infection, which is an effective and feasible therapeutic method for the treatment of EB virus.
research method
1. pharmacodynamic experiments: the mice were weighed and numbered. The mice were randomly divided into experimental group, standard control group and blank control group. The experimental group was divided into high dose group, medium dose group and low dose group. The experimental group was administered by the experimental scheme, the chicken red cell suspension was injected, the mice were killed and the abdominal cavity liquid 0.5ml was extracted, dripping on the clean slides, each piece of 0.5ml, and each small tablet. 2 slices of rat. Then put in the enamel box with wet gauze, hatch for half an hour at 37. Take the glass tap water to wash the wall cells and dry. After 5 minutes of acetone methanol solution (1:1),.Giemsa-W right is taken out for 3~5 minutes, and the tap water is washed and dried, and 200 macrophages are counted and counted to calculate the phagocytic finger. The number (phagocytic index = /200 macrophage of the total number of chicken red cells being phagocytosis) and the percentage of phagocytosis (phagocytic percentage = /200 macrophages, macrophages phagocytosis of chicken red cells). All data were recorded and analyzed by variance analysis.
2. clinical effect observation: the cases were taken into the standard and exclusion criteria, and the patients with EB virus infection or EB virus infection combined with Qi deficiency and fever nasopharyngitis were randomly assigned into two groups, one group was Qi Ju tea bag treatment group, oral tea bag tea, one time 2 packs, 15 minutes after water immersion, 2 times a day; one group was empty. White control group, that is, no clinical intervention group. After 3 weeks, the EB virus was re examined and the EB virus negative condition was observed.
3. statistical analysis: counting data using chi square test, measurement data using t test, all the data are input to the computer database, SPSS13.0 statistical software is used for statistical analysis, the test level is a=0.05. for the two groups of patients before and after the EB virus conversion rate is compared, the comparison between groups, compare the two groups of curative effect.
Research results
1. pharmacodynamic experiments
Statistical phagocytosis rate (phagocytic percentage = /200 macrophage macrophages phagocytosis of chicken red cells) and phagocytic index (phagocytic index = /200 macrophages of the total number of chicken red cells being phagocytic). The 5 groups were analyzed by variance, Fang Chaqi test P0.05, homogeneity of variance, the comparability among the groups. P0.05, experimental group, standard control group There were differences in the comparison of phagocytic rate and phagocytic index between the control group and the blank control group. The comparison between the experimental group and the standard control group and the blank control group was different. There were differences between the middle dose group and the high dose group, the low dose group, the standard control group and the blank control group, with statistical meaning, indicating that the Qi chrysanthemum tea bag had the enhancement of the non specificity of the mice. Immunity.
2. clinical curative effect observation
All cases were from the Department of ENT of the First Affiliated Hospital of Guangzhou University of Chinese Medicine from March 2011 to April 2012. 56 cases were randomly divided into two groups, including 26 cases of Qi chrysanthemum tea bag treatment group, 18 cases with rhinopharyngitis related symptoms, 8 cases without amalgamative symptoms. 30 cases in the blank control group, 22 cases with nasopharyngitis related symptoms, without complication. According to the statistical analysis, there was no difference between the control group and the treatment group before the treatment. There was no difference between the control group and the treatment group before the treatment. P0.05, the basic information of the two groups was balanced and comparable. In this study, 26 patients in the treatment group had no obvious adverse reaction during the period of taking Qi chrysanthemum tea bags. 3 weeks after the reexamination of the serum EB virus, 12 of them turned Yin and the conversion rate was 46.2%. 5 cases of the control group rechecked the sera EB virus turned negative, and the negative rate was 16.7%. The negative rate of the EB virus infection between the two groups was compared by the chi square test, and the statistical significance was compared. 18 cases in the treatment group were combined with the nasopharyngitis related symptoms to recheck the serum EB virus 9 cases turn Yin and turn to the negative. There were 3 cases of negative rate of 50%.8 without symptomatic patients. The negative rate was 3 of the 22 cases in the 37.5%. control group with nasopharyngitis related symptoms in 3 cases, and the negative rate was 2 cases in 16.7%.8 without symptomatic patients, and the negative rate was 25.0%. through the chi square test. Statistical significance. After t test, the difference between the two groups in total integral difference, main difference, secondary difference and sign difference were all P0.01, with significant difference.
research conclusion
The Qi chrysanthemum tea bag can enhance the non specific immune function of the mice, can promote the EB virus to turn to the Yin, and can improve the clinical symptoms of the EB virus infected patients.
【學(xué)位授予單位】：廣州中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R273;R739.63

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