本文選題：幼年型喉乳頭狀瘤 + 人乳頭狀瘤病毒�。� 參考：《廣西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：研究5-氨基酮戊酸光動力學(xué)(ALA-PDT)對裸鼠幼年型喉乳頭狀瘤移植瘤的殺傷作用。 方法：建立裸鼠幼年型喉乳頭狀瘤移植瘤模型,在腫瘤局部應(yīng)用5～氨基酮戊酸溶液,3小時后用波長為630nm的氮氖激光照射腫瘤10min,照射劑量為90J/Cm2。輻照后,每天觀察腫瘤變化情況,記錄測量腫瘤體積并計算相對腫瘤增殖率T/C,并設(shè)單用5-ALA,單用PDT,陰性對照組,觀察處理后的腫瘤的組織形態(tài)學(xué)變化。采用原位雜交、TUNEL檢測、透射電鏡的方法,研究5-氨基酮戊酸光動力對喉乳頭狀瘤組織細胞凋亡情況的影響,應(yīng)用熒光定量聚合酶鏈反應(yīng)(polymerase chain reaction,PCR)法檢測腫瘤組織中的HPV6/11含量。 結(jié)果：成功建立幼年型喉乳頭狀瘤手術(shù)標(biāo)本組織塊移植瘤模型。5-ALA-PDT治療組的腫瘤組織均可使腫瘤顯著縮小,腫瘤表面可見壞死,TUNEL檢測及透射電鏡均見腫瘤組織有廣泛凋亡及壞死,24h及48h的相對腫瘤增值率分別是11.6%和17.2%,p0.05,熒光定量PCR檢測病毒含量明顯減少。生理鹽水對照組、5-ALA組、PDT組對腫瘤無顯著作用。 結(jié)論：5-ALA-PDT對幼年型喉乳頭狀瘤的生長有顯著的抑制作用。
[Abstract]:Aim: to study the killing effect of 5 aminolevulinic acid (ALA-PDT) on juvenile laryngeal papilloma in nude mice. Methods: the transplanted tumor model of juvenile laryngeal papilloma in nude mice was established. After 3 hours of local application of 5-aminolevulinic acid solution, the tumor was irradiated with 630nm laser for 10 min at a dose of 90 J / cm ~ 2. After irradiation, tumor changes were observed daily, tumor volume was measured and relative tumor proliferation rate (T / C) was calculated. The histomorphologic changes of the treated tumors were observed by 5-ALA-PDT alone and PDT-negative control group. The effect of 5-aminolevulinic acid on apoptosis of human laryngeal papilloma tissue was studied by in situ hybridization Tunel and transmission electron microscopy. The HPV6 / 11 content in tumor tissue was detected by fluorescence quantitative polymerase chain reaction (FQ-PCR) and (polymerase chain reaction-polymerase chain reaction (FQ-PCR). Results: the tumor tissue of the group treated with 5-ALA-PDT could significantly reduce the tumor size, and the model of transplanted tumor was established successfully in the operation specimen of juvenile laryngeal papilloma. TUNEL-mediated dUTP Nick end labeling (Tunel) and transmission electron microscopy (TEM) showed that the relative tumor proliferation rates of 24 h and 48 h were 11.6% and 17.2%, respectively, and the amount of virus detected by fluorescence quantitative PCR was significantly decreased. Normal saline control group, 5-ALA group, PDT group had no significant effect on tumor. Conclusion the growth of juvenile laryngeal papilloma was significantly inhibited by 5-ALA-PDT.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R739.65

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