本文選題：MiR-a + 鼻咽腫瘤�。� 參考：《臨床耳鼻咽喉頭頸外科雜志》2016年15期

【摘要】：目的:探討MiR-148a在鼻咽癌細(xì)胞中的表達(dá)水平變化及其對(duì)鼻咽癌腫瘤細(xì)胞生物學(xué)功能的影響。方法:采用Real-time PCR方法檢測(cè)MiR-148a在鼻咽癌細(xì)胞系CNE2中的表達(dá)水平變化,并通過(guò)MTT實(shí)驗(yàn)、克隆形成實(shí)驗(yàn)和流式細(xì)胞術(shù)檢測(cè)MiR-148a表達(dá)變化對(duì)鼻咽癌細(xì)胞的增殖和凋亡影響。通過(guò)生物信息學(xué)軟件分析預(yù)測(cè)MiR-148a的下游靶向調(diào)控基因表皮生長(zhǎng)因子受體(EGFR),Real-time PCR和Western blot檢測(cè)EGFR表達(dá)水平變化。結(jié)果:相對(duì)于正常鼻咽癌上皮組織細(xì)胞,MiR-148a在鼻咽癌細(xì)胞系CNE2中的表達(dá)水平顯著降低。MTT實(shí)驗(yàn)、克隆形成實(shí)驗(yàn)和流式細(xì)胞術(shù)檢測(cè)發(fā)現(xiàn)MiR-148a能夠抑制細(xì)胞增殖,并促進(jìn)細(xì)胞的凋亡。Real-time PCR和Western blot檢測(cè)結(jié)果表明MiR-148a可降低EGFR的表達(dá)水平。結(jié)論:MiR-148a能夠影響鼻咽癌細(xì)胞的增殖和凋亡,很有可能參與了鼻咽癌的發(fā)生發(fā)展過(guò)程。
[Abstract]:Aim: to investigate the expression of MiR-148a in nasopharyngeal carcinoma (NPC) cells and its effect on the biological function of nasopharyngeal carcinoma (NPC) cells. Methods: Real-time PCR was used to detect the expression of MiR-148a in nasopharyngeal carcinoma cell line CNE2. MTT assay, colony formation assay and flow cytometry were used to detect the effect of MiR-148a expression on the proliferation and apoptosis of nasopharyngeal carcinoma cells. The changes of EGFR expression level were predicted by bioinformatics software analysis, and EGFR expression level was detected by EGFR real-time PCR and Western blot, which was the downstream target regulatory gene of MiR-148a. Results: compared with normal nasopharyngeal carcinoma epithelial cells, the expression of MiR-148a in nasopharyngeal carcinoma cell line CNE2 was significantly decreased by MTT assay. Clone formation assay and flow cytometry showed that MiR-148a could inhibit the proliferation of nasopharyngeal carcinoma cell line CNE2. The results of cell apoptosis. Real-time PCR and Western blot analysis showed that MiR-148a could decrease the expression of EGFR. ConclusionMiR-148a can affect the proliferation and apoptosis of nasopharyngeal carcinoma cells and may be involved in the genesis and development of nasopharyngeal carcinoma.
【作者單位】： 陸軍總醫(yī)院263臨床部耳鼻咽喉頭頸外科;
【分類(lèi)號(hào)】：R739.63

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