本文選題：視網(wǎng)膜新生血管 + 酪氨酸激酶抑制劑�。� 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:研究酪氨酸激酶抑制劑(Genistein)在高氧誘導(dǎo)小鼠視網(wǎng)膜新生血管形成中的作用。方法:應(yīng)用隨機(jī)數(shù)字表法將36只7日齡清潔級(jí)C57BL/6J小鼠隨機(jī)分為:模型對(duì)照組、Gen組、DMSO組、正常組,共四組,每組9只,18眼。前三組小鼠為7日齡小鼠在氧體積分?jǐn)?shù)為(75±2)%的密閉氧箱內(nèi)生長(zhǎng)5天后,返回到正常環(huán)境;正常組為自然環(huán)境中生長(zhǎng)的小鼠。模型對(duì)照組和正常組小鼠不給藥物處理,Gen組和DMSO組的12天小鼠,玻璃體腔內(nèi)分別注射Genistein(溶于DMSO,400mg/L)和DMSO各1μl。繼續(xù)在正常環(huán)境下培養(yǎng)小鼠5天后,即小鼠17日齡時(shí),每組兩只小鼠4眼行視網(wǎng)膜血管熒光造影鋪片觀察視網(wǎng)膜血管的形態(tài),各組剩余小鼠全部處死,獲取視網(wǎng)膜,應(yīng)用real-time PCR法,檢測(cè)小鼠視網(wǎng)膜中VEGF和b FGFm RNA的表達(dá)量(2-ΔΔCt);Western blot法,檢測(cè)各組小鼠視網(wǎng)膜中VEGF和b FGF蛋白的相對(duì)表達(dá)量(VEGF和b FGF/β-actin)。結(jié)果:在模型對(duì)照組和DMSO組中,小鼠視網(wǎng)膜中VEGF和b FGFm RNA的表達(dá)水平分別為(0.64±0.25和21.40±3.07,0.37±0.23和17.22±2.63),明顯高于正常組和Gen組(0.04±0.02和0.67±0.23,0.03±0.02和0.52±0.25),差異有統(tǒng)計(jì)學(xué)意義(P0.05)。經(jīng)兩兩比較,模型對(duì)照組和DMSO組、正常組和Gen組比較差異無統(tǒng)計(jì)學(xué)意義(P0.05),其余各組兩兩比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。模型對(duì)照組和DMSO組小鼠視網(wǎng)膜中,VEGF和b FGF蛋白的表達(dá)水平分別為(1.01±0.05和0.97±0.06,1.06±0.07和1.03±0.08),明顯高于正常組和Gen組(0.52±0.05和0.56±0.05,0.73±0.05和0.76±0.07),差異有統(tǒng)計(jì)學(xué)意義(P0.05)。經(jīng)兩兩比較,VEGF,b FGF蛋白在模型對(duì)照組和DMSO組中,差異無統(tǒng)計(jì)學(xué)意義(P0.05),其余兩兩相比,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。視網(wǎng)膜熒光造影鋪片顯示:模型對(duì)照組和DMSO組血管分層和分支較多,血管走行紊亂,新生血管簇明顯;Gen組小鼠視網(wǎng)膜血管分層、分支較少,視網(wǎng)膜血管走行相對(duì)清晰,未見明顯新生血管;正常組視網(wǎng)膜血管走形整齊,清晰,未見新生血管簇。結(jié)論:1.高氧誘導(dǎo)小鼠視網(wǎng)膜新生血管中VEGF和b FGF高表達(dá);2.Genistein能抑制視網(wǎng)膜新生血管中VEGF和b FGFm RNA及蛋白的生成;3.Genistein可以抑制視網(wǎng)膜新生血管的形成。
[Abstract]:Aim: to study the role of tyrosine kinase inhibitor Genistein in retinal neovascularization induced by hyperoxia in mice. Methods: Thirty-six C57BL / 6J mice of 7 days old were randomly divided into four groups: model control group (Gen group), normal group (n = 9, n = 18) and control group (n = 9, n = 18). The first three groups were 7-day-old mice, which grew in a closed oxygen box with 75 鹵2% oxygen volume fraction for 5 days, then returned to normal environment, while the normal group was the mice growing in the natural environment. The mice in the model control group and the normal group were not treated with drug treatment for 12 days. Genistein (400 mg / L dissolved in DMSOO) and DMSO were injected intravitreally into the vitreous body of mice for 12 days, respectively. After continuing to culture mice in normal environment for 5 days, that is, at the age of 17 days, four eyes of two mice in each group underwent retinal angiography to observe the morphology of retinal vessels, and all the remaining mice in each group were killed to obtain retina. The expression of VEGF and bFGFm mRNA in the retina of mice was detected by real-time polymerase chain reaction. The relative expression of VEGF and bFGF protein in the retina of each group was detected by Western blot method. Results: in the model control group and DMSO group, the expression levels of VEGF and bFGFm mRNA in the retina of mice were 0.64 鹵0.25 and 21.40 鹵3.07 鹵0.37 鹵0.23 and 17.22 鹵2.63, respectively, which were significantly higher than those in the normal group and Gen group (0.04 鹵0.02 and 0.67 鹵0.230.03 鹵0.02 and 0.52 鹵0.25, respectively). There was no significant difference between model control group and DMSO group, normal group and Gen group. The expression levels of VEGF and bFGF in the retina of model control group and DMSO group were 1.01 鹵0.05 and 0.97 鹵0.06 鹵0.07 鹵0.07 and 1.03 鹵0.08, respectively, which were significantly higher than those in normal group and Gen group, 0.52 鹵0.05 and 0.56 鹵0.05 鹵0.73 鹵0.05 and 0.76 鹵0.07, respectively. There was no significant difference in VEGFb FGF protein between model control group and DMSO group, but there was significant difference between the other two groups. Fluorescein angiography showed that in the model control group and DMSO group, there were more blood vessel layers and branches, and the blood vessels in the neovascularization group were disordered, the retinal blood vessels in the neovascularization group were obviously stratified, the branches were less, and the retinal blood vessels were relatively clear. There was no obvious neovascularization in the normal group, and no neovascularization was found in the normal group. Conclusion 1. High expression of VEGF and bFGF in retinal neovascularization induced by hyperoxia. 2. Genistein can inhibit the production of VEGF and bFGFm RNA and protein in retinal neovascularization. 3. Genistein can inhibit the formation of retinal neovascularization.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R774.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 ;Effects of phytoestrogen genistein on myocardial ischemia/reperfusion injury and apoptosis in rabbits[J];Acta Pharmacologica Sinica;2004年03期

2 ;Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo[J];Acta Pharmacologica Sinica;2004年03期

3 ;Blocking effects of genistein on cell proliferation and possible mechanism in human gastric carcinoma[J];World Journal of Gastroenterology;2005年01期

4 ;Effect of genistein on mouse blastocyst development in vitro[J];Acta Pharmacologica Sinica;2007年02期

5 ;Apoptosis of human primary gastric carcinoma cells induced by genistein[J];World Journal of Gastroenterology;2004年12期

6 ;Genistein stimulates osteoblastic differentiation via p38 MAPK-Cbfa1 pathway in bone marrow culture[J];Acta Pharmacologica Sinica;2007年10期

7 凌航;張文敏;;genistein增強(qiáng)腫瘤化療藥敏感性以及分子機(jī)制的研究進(jìn)展[J];醫(yī)學(xué)綜述;2010年01期

8 ;siRNA of ADAM17 gene induces apoptosis,proliferation inhibition and enhances the effects of genistein on HepG2 cells[J];Journal of Nanjing Medical University;2009年02期

9 ;Inhibitory effects of genistein on metastasis of human hepatocellular carcinoma[J];World Journal of Gastroenterology;2009年39期

10 WANG Ying;ZHANG Joe;GENG Xing-chao;LI Bo;WANG Xue;;A high-throughput screening method of cell transformation assay in Bhas 42 cells and the research on genistein[J];中國(guó)藥理學(xué)與毒理學(xué)雜志;2013年03期

相關(guān)會(huì)議論文 前10條

1 曹川;李世榮;戴霞;陳艷清;馮智;覃霞;趙云;吳軍;;Genistein對(duì)增生性瘢痕成纖維細(xì)胞TPK-Ras-MAPK信號(hào)通路的影響[A];第六屆全國(guó)燒傷救治專題研討會(huì)論文匯編[C];2009年

2 ;The role of caveolin1 and sprouty1 in genistein's regulation of vascular smooth muscle cell proliferation[A];中國(guó)生理學(xué)會(huì)第23屆全國(guó)會(huì)員代表大會(huì)暨生理學(xué)學(xué)術(shù)大會(huì)論文摘要文集[C];2010年

3 ;A novel anti-cancer effect of genistein:reversal of epithelial mesenchymal transition in prostate cancer cells[A];第十五屆全國(guó)泌尿外科學(xué)術(shù)會(huì)議論文集[C];2008年

4 ;Neuroprotective effects of genistein against 6-OHDA-induced neurotoxicity in SK-N-SH cells[A];Proceedings of the 7th Biennial Meeting and the 5th Congress of the Chinese Society for Neuroscience[C];2007年

5 ;IGF-I receptor signaling pathway is involved in the neuroprotective effect of genistein in the mice model of Parkinson's disease[A];Proceedings of the 8th Biennial Conference of the Chinese Society for Neuroscience[C];2009年

6 王斌;鄒穎;袁志蘭;肖繼皋;;Genistein對(duì)缺氧和氯化鈷誘導(dǎo)的兔視網(wǎng)膜色素上皮細(xì)胞血管內(nèi)皮生長(zhǎng)因子表達(dá)的影響[A];中國(guó)藥理學(xué)會(huì)第八次全國(guó)代表大會(huì)暨全國(guó)藥理學(xué)術(shù)會(huì)議論文摘要匯編[C];2002年

7 WANG Ying;ZHANG Joe;GENG Xing-chao;LI Bo;WANG Xue;;A high-throughput screening method of cell transformation assay in Bhas 42 cells and the research on genistein[A];2013年（第三屆）中國(guó)藥物毒理學(xué)年會(huì)暨藥物非臨床安全性評(píng)價(jià)研究論壇論文摘要[C];2013年

8 WANG Ying;ZHANG Joe;GENG Xing-chao;LI Bo;WANG Xue;;A high-throughput screening method of cell transformation assay in Bhas 42 cells and the research on genistein[A];中國(guó)藥理學(xué)與毒理學(xué)雜志(2013年6月第27卷第3期)[C];2013年

9 潘琪琦;周容;劉曉玲;;改良的可定量氧誘導(dǎo)法建立視網(wǎng)膜新生血管小鼠模型[A];中國(guó)眼底病論壇·全國(guó)眼底病專題學(xué)術(shù)研討會(huì)論文匯編[C];2008年

10 劉軍;曾平;賴銘瑩;廖明怡;呂娟;;Vogt-Koyanagi-Harada綜合征繼發(fā)視網(wǎng)膜新生血管的中西醫(yī)結(jié)合治療[A];全國(guó)第九次中醫(yī)、中西醫(yī)結(jié)合眼科學(xué)術(shù)年會(huì)論文匯編[C];2010年

相關(guān)重要報(bào)紙文章 前2條

1 新華;視網(wǎng)膜自我修復(fù)研究有突破[N];福建科技報(bào);2007年

2 新訊;視網(wǎng)膜自我修復(fù)研究有突破[N];醫(yī)藥經(jīng)濟(jì)報(bào);2007年

相關(guān)博士學(xué)位論文 前10條

1 Asghar Ali Kamboh;[D];南京農(nóng)業(yè)大學(xué);2012年

2 趙慶霞;CIP2A在genistein和lapatinib抗乳腺癌中的作用[D];鄭州大學(xué);2016年

3 段佳良;硫氧還蛋白相互作用蛋白在增殖性糖尿病視網(wǎng)膜病變中的作用及機(jī)制研究[D];河北醫(yī)科大學(xué);2017年

4 鄒賀;整合素連接激酶與視網(wǎng)膜新生血管的實(shí)驗(yàn)性研究[D];吉林大學(xué);2007年

5 顏春洪;Genistein抗惡性腫瘤作用及其機(jī)理研究[D];中國(guó)協(xié)和醫(yī)科大學(xué);1997年

6 王偉;內(nèi)皮抑素基因轉(zhuǎn)移抑制視網(wǎng)膜新生血管的實(shí)驗(yàn)研究[D];青島大學(xué);2003年

7 胡曼;1-磷酸鞘氨醇受體2抑制劑對(duì)小鼠視網(wǎng)膜新生血管抑制作用的研究[D];中南大學(xué);2012年

8 白熠洲;重組人血管內(nèi)皮抑素對(duì)小鼠氧致視網(wǎng)膜新生血管的抑制作用[D];北京協(xié)和醫(yī)學(xué)院;2010年

9 MUHAMMAD FAROOQ IQBAL;[D];南京農(nóng)業(yè)大學(xué);2009年

10 晏穎;15-脂氧合酶-1在缺氧導(dǎo)致的視網(wǎng)膜新生血管性疾病中的作用機(jī)制研究[D];武漢大學(xué);2012年

相關(guān)碩士學(xué)位論文 前10條

1 賀榮華;酪氨酸激酶抑制劑（Genistein）在小鼠視網(wǎng)膜新生血管形成中的作用[D];山西醫(yī)科大學(xué);2017年

2 阮露;Ang/Tie2與VEGF/VEGFR信號(hào)通路對(duì)視網(wǎng)膜新生血管及血管發(fā)育的作用研究[D];復(fù)旦大學(xué);2014年

3 王夢(mèng)嬌;胰島素樣生長(zhǎng)因子1對(duì)人視網(wǎng)膜血管內(nèi)皮細(xì)胞作用和機(jī)制的研究[D];蘇州大學(xué);2016年

4 劉鵬飛;促紅細(xì)胞生成素受體抗體抑制小鼠視網(wǎng)膜新生血管的實(shí)驗(yàn)研究[D];第四軍醫(yī)大學(xué);2008年

5 種澤龍;2型糖尿病視網(wǎng)膜新生血管的危險(xiǎn)因素研究[D];天津醫(yī)科大學(xué);2009年

6 萬磊;乙酰肝素酶與鼠視網(wǎng)膜新生血管的實(shí)驗(yàn)研究[D];青島大學(xué);2009年

7 劉鶴南;腎素—血管緊張素系統(tǒng)阻滯劑抑制視網(wǎng)膜新生血管的實(shí)驗(yàn)研究[D];中國(guó)醫(yī)科大學(xué);2010年

8 楊柳;視網(wǎng)膜新生血管動(dòng)物模型的制作及血管內(nèi)皮生長(zhǎng)因子的表達(dá)[D];青島大學(xué);2003年

9 解孝鋒;血管抑素對(duì)視網(wǎng)膜新生血管抑制作用的實(shí)驗(yàn)研究[D];山東中醫(yī)藥大學(xué);2004年

10 凌航;genistein聯(lián)合5-Fu促人結(jié)腸癌細(xì)胞凋亡及相關(guān)機(jī)制的研究[D];福建醫(yī)科大學(xué);2010年

，

本文編號(hào)：2010504