本文選題：辛伐他汀 + 視神經(jīng)夾傷　； 參考：《眼科新進(jìn)展》2017年12期

【摘要】：目的探討辛伐他汀對視神經(jīng)損傷后(optic nerve crush,ONC)視網(wǎng)膜神經(jīng)節(jié)細(xì)胞(retinal ganglion cell,RGC)的保護(hù)作用。方法將60只昆明鼠分為正常組、假損傷組、損傷組和辛伐他汀修復(fù)組。正常組不作任何處理,假損傷組只暴露視神經(jīng)不損傷,損傷組行左眼視神經(jīng)夾傷手術(shù)后,玻璃體內(nèi)注射平衡溶劑(50 mg·m L~(-1)乙醇和1 mol·L~(-1)Na OH的混合溶劑,并用1 mol·L~(-1)鹽酸激活并調(diào)節(jié)p H值為7.2),辛伐他汀修復(fù)組行左眼視神經(jīng)夾傷手術(shù)后,玻璃體腔注射不同濃度辛伐他汀(0.5g·L~(-1)、1.0 g·L~(-1)、1.5 g·L~(-1))。Brn3a免疫熒光染色、HE染色、甲苯胺藍(lán)染色檢測RGC凋亡及視神經(jīng)的病理變化。結(jié)果術(shù)后7 d,損傷組RGC凋亡明顯,RGC存活率降低至(35.1±3.9)%,RGC層到外核層厚度由(123.13±1.04)μm降低至(97.48±2.33)μm,與正常組相比,差異有統(tǒng)計(jì)學(xué)意義(P0.01);視神經(jīng)纖維束狀排列消失,神經(jīng)膠質(zhì)細(xì)胞軸突聚集成團(tuán),腫脹變形,退行性病變嚴(yán)重,與正常組相比差異顯著。玻璃體內(nèi)注射1.0 g·L~(-1)辛伐他汀后,RGC存活率增加至(76.3±3.7)%(P0.05),RGC層到外核層厚度增加至(111.39±4.06)μm,與損傷組相比,差異有統(tǒng)計(jì)學(xué)意義(P0.01),視神經(jīng)退行性病變明顯改善,膠質(zhì)細(xì)胞軸突和神經(jīng)纖維束狀結(jié)構(gòu)趨于正常。結(jié)論玻璃體內(nèi)注射辛伐他汀可以減緩視神經(jīng)退行性病變,提高RGC存活率。
[Abstract]:Objective to investigate the protective effect of simvastatin on retinal ganglion cells (RGCs) of retinal ganglion cells (RGCs) after optic nerve injury. Methods 60 Kunming rats were divided into normal group, sham injury group, injury group and simvastatin repair group. No treatment was given in the normal group, but only the optic nerve was exposed in the sham injury group. After the operation on the left eye optic nerve, the mixture of ethanol and 1 mol L~(-1)Na OH was injected intravitreally into the vitreous. The left optic nerve injury was performed in the simvastatin repair group. 0.5 g / L simvastatin was injected into the vitreous body and 1.0 g / L of simvastatin was injected into the vitreous cavity and the pH value was adjusted to 7.2g / h. He staining was performed with different concentrations of simvastatin (0.5 g / L) / L ~ (-1) / L ~ (-1) and 1.0 g / L ~ (+) ~ (-1) / L ~ (-1) / L ~ (-1) / L ~ (-1) ~ (-1). The apoptosis of RGC and the pathological changes of optic nerve were detected by toluidine blue staining. Results on the 7th day after operation, the survival rate of RGC decreased to 35.1 鹵3.9 渭 m from 123.13 鹵1.04 渭 m to 97.48 鹵2.33 渭 m in the injured group. Swelling, deformability and degenerative lesion were serious, and the difference was significant compared with the normal group. The survival rate of RGC increased to 76.3 鹵3.7m after intravitreous injection of 1.0 g / L ~ (-1) simvastatin, and the thickness of RGC layer to outer nuclear layer increased to 111.39 鹵4.06 渭 m. Compared with the injured group, the difference was statistically significant (P 0.01), and the optic neurodegenerative lesion was significantly improved. Glial axons and nerve fiber bundles tend to be normal. Conclusion Intravitreous injection of simvastatin can reduce the degeneration of optic nerve and improve the survival rate of RGC.
【作者單位】： 蘭州大學(xué);
【基金】：國家自然科學(xué)基金資助(編號:31471953) 甘肅省自然科學(xué)基金資助(編號:17JR5RA220)~~
【分類號】：R774
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本文編號：1971204