本文選題：IL-1β + 角膜上皮��； 參考：《青島大學(xué)》2012年碩士論文

【摘要】：目的：觀察IL-1β在大鼠角膜上皮抗曲霉菌感染固有免疫階段的表達(dá),阻斷Dectin-1受體后,觀察固有免疫階段Dectin-1受體與IL-1β的關(guān)系。方法：健康的Wistar大鼠90只,雌雄不限,在刮除上皮的大鼠角膜涂布以煙曲霉菌菌絲并覆蓋自制角膜接觸鏡,建立角膜真菌感染模型。隨機分5組,A組6只為空白對照組；B組30只為真菌感染模型組,D組12只為模型阻斷組,C組30只、E組12只分別為損傷對照組。均取右眼為實驗眼,B組建立真菌性角膜炎模型,損傷對照組覆蓋角膜接觸鏡并滴入PBS液；D組給予Dectin-1受體抗體后建立模型,左眼給予PBS后建立模型作為對照。建模后β、C組分別于4h,8h,16h,24h,D、E組于24h取眼球角膜上皮,運用免疫組織化學(xué)和RT-PCR技術(shù)檢測角膜上皮中Dectin-1受體和IL-1β的表達(dá)。結(jié)果：IL-1β在空白對照組和條件對照組的角膜上皮呈微量表達(dá),在真菌性角膜炎模型建立后IL-1β在角膜上皮中的表達(dá)量隨病變進(jìn)展逐漸增高,建模后16h至24h緩慢增高,各時間點1L-1β表達(dá)量的差異有統(tǒng)計學(xué)意義(P0.01)。Dectin-1受體在空白對照組角膜上皮呈基礎(chǔ)表達(dá),在真菌性角膜炎模型建立后Dectin-1受體表達(dá)量明顯增多,差異具有顯著性(P0.01)。阻斷Dectin-1受體后真菌性角膜炎建模24h的IL-1β表達(dá)量明顯低于未阻斷Dectin-1受體組,差異具有顯著性(P0.01)。結(jié)論：1.顯微鏡下觀察大鼠角膜水腫,渾濁,潰瘍形成,HE染色見角膜中性粒細(xì)胞浸潤,角膜組織刮取物行菌落培養(yǎng)證實大鼠真菌性角膜炎動物模型建模成功。2.IL-1β表達(dá)在真菌性角膜炎上皮中,其表達(dá)量隨病變發(fā)展逐漸增高,提示IL-1β參與真菌性角膜炎的發(fā)生發(fā)展,是真菌性角膜炎病變中的敏感炎性因子。3. Dectin-1受體在空白對照組呈基礎(chǔ)表達(dá),在建模后Dectin-1表達(dá)量增多,Dectin-1抗體封閉Dectin-1受體后,能顯著減少角膜上皮中IL-1β的量。這提示Dectin-1參與角膜上皮對煙曲霉菌菌絲的識別和反應(yīng)。
[Abstract]:Aim: to observe the expression of IL-1 尾 in the innate immune phase of rat corneal epithelial anti-Aspergillus infection, and to investigate the relationship between Dectin-1 receptor and IL-1 尾 after blocking the Dectin-1 receptor. Methods: 90 healthy Wistar rats, male and female, were coated with Aspergillus fumigatus mycelium and self-made contact lens to establish the model of corneal fungal infection. Five groups were randomly divided into 5 groups: control group (n = 6), group B (n = 30), model group B (n = 30), group D (n = 12), model group C (n = 30), group E (n = 12), injury control group (n = 12). The model of fungal keratitis was established in the right eye as experimental eye group B, and the model was established in group D after Dectin-1 receptor antibody was given to the injured control group after covering the cornea contact lens and dripping into PBS solution. The model was established after PBS was given to the left eye as control group. After modeling, the corneal epithelium of group 尾 C was collected at 4 h, 8 h, 16 h and 24 h, respectively. The expression of Dectin-1 receptor and IL-1 尾 in corneal epithelium was detected by immunohistochemistry and RT-PCR technique. Results the expression of IL-1 尾 in the corneal epithelium of the control group and the control group was slight. After the establishment of the fungal keratitis model, the expression of IL-1 尾 in the corneal epithelium increased gradually with the progression of the lesion, and increased slowly from 16 hours to 24 hours after the establishment of the model. There were significant differences in the expression of 1L-1 尾 between different time points. The expression of 1L-1 尾 in the corneal epithelium of the blank control group showed a basic expression. After the establishment of the fungal keratitis model, the expression of Dectin-1 尾 increased obviously, and the difference was significant (P 0.01). The expression of IL-1 尾 in fungal keratitis model 24 h after blocking Dectin-1 receptor was significantly lower than that in unblocked Dectin-1 receptor group (P 0.01). Conclusion 1. Under microscope, corneal edema, turbidity, ulcer formation and neutrophil infiltration were observed in rats. Colony culture of corneal tissue scraping confirmed that the rat model of fungal keratitis was successfully established. 2. The expression of IL-1 尾 in the epithelium of fungal keratitis increased with the development of the lesion, suggesting that IL-1 尾 was involved in the occurrence and development of fungal keratitis. It is a sensitive inflammatory factor in fungal keratitis. The expression of Dectin-1 receptor was basic in blank control group. After modeling, the increase of Dectin-1 expression and the blocking of Dectin-1 receptor by Dectin-1 antibody could significantly reduce the amount of IL-1 尾 in corneal epithelium. This suggests that Dectin-1 is involved in the recognition and response of corneal epithelium to Aspergillus fumigatus hyphae.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R772.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 ;Effects of COX-2 inhibitor NS-398 on IL-10 expression in rat fungal keratitis[J];International Journal of Ophthalmology(English Edition);2011年02期

2 ;The roles of surfactant protein D during Aspergillus fumigatus infection in human corneal epithelial cells[J];International Journal of Ophthalmology(English Edition);2012年01期

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本文編號：1970464