本文選題：干眼 + omega-�。� 參考：《眼科新進(jìn)展》2016年09期

【摘要】：目的探討omega-3治療苯扎氯銨誘導(dǎo)的大鼠干眼的療效及其可能機(jī)制。方法45只6~8周齡SD大鼠,隨機(jī)分為3組,每組15只。生理鹽水組用生理鹽水灌胃,橄欖油組用橄欖油灌胃,omega-3組用omega-3灌胃,連續(xù)2個月。2個月后,3組分別接受15 d的苯扎氯銨誘導(dǎo)干眼,干眼誘導(dǎo)期間大鼠灌胃同前。在干眼誘導(dǎo)當(dāng)天、第5天、第10天、第15天觀察淚液分泌量、淚膜破裂時間(tear break-up time,BUT)和角膜熒光素染色評分,干眼誘導(dǎo)第15天行大鼠角膜和結(jié)膜組織的HE染色。結(jié)果干眼誘導(dǎo)第15天omega-3組淚液分泌量比橄欖油組和生理鹽水組明顯增多,差異均有統(tǒng)計學(xué)意義(P=0.023、0.027)。干眼誘導(dǎo)第10天、第15天omega-3組BUT較橄欖油組和生理鹽水組明顯變長,差異均有統(tǒng)計學(xué)意義(均為P0.05)。干眼誘導(dǎo)第15天omega-3組角膜熒光素染色評分比橄欖油組和生理鹽水組明顯減少,差異均有統(tǒng)計學(xué)意義(P=0.012、0.015)。干眼誘導(dǎo)第15天omega-3組角膜、結(jié)膜組織與正常角膜、結(jié)膜組織相似。橄欖油組和生理鹽水組角膜上皮細(xì)胞層次明顯增加,表層細(xì)胞不規(guī)則缺失,基底細(xì)胞排列紊亂,基質(zhì)層少許水腫;結(jié)膜上皮結(jié)構(gòu)不完整,排列不規(guī)則,結(jié)膜上皮細(xì)胞增生,基底層細(xì)胞排列不整齊,可見炎性細(xì)胞浸潤。結(jié)論omega-3能有效抑制淚液分泌量,增強(qiáng)淚膜穩(wěn)定性,保護(hù)角膜和結(jié)膜組織。
[Abstract]:Objective to investigate the effect and possible mechanism of omega-3 on dry eye of rats induced by benzalkonium chloride. Methods 45 SD rats aged 6 weeks and 8 weeks were randomly divided into 3 groups with 15 rats in each group. The normal saline group was fed with normal saline, the olive oil group with olive oil and the omega-3 group with omega-3 for 2 months. After 2 months, the rats in the three groups were treated with benzalkonium chloride for 15 days to induce the dry eye, and the rats in the olive oil group were given the same intragastric perfusion during the dry eye induction period. On the day of dry eye induction, 5 days, 10 days, 15 days, the amount of tear secretion, tear film rupture time (tear break-up time But) and corneal fluorescein staining score were observed, and the HE staining of cornea and conjunctiva of rats was performed on the 15th day after dry eye induction. Results on the 15th day of dry eye induction, the amount of tear secretion in omega-3 group was significantly higher than that in olive oil group and normal saline group, and the difference was statistically significant. The BUT of omega-3 group was significantly longer than that of olive oil group and normal saline group on the 10th and 15th day of dry eye induction, and the difference was statistically significant (P 0.05). On the 15th day of dry eye induction, the corneal fluorescein staining score in omega-3 group was significantly lower than that in olive oil group and normal saline group, and the difference was statistically significant. In omega-3 group, the conjunctival tissue was similar to the normal cornea and conjunctival tissue on the 15th day after dry eye induction. In the olive oil group and saline group, the corneal epithelial cells increased significantly, the surface cells were irregularly absent, the basal cells were arranged in disorder, the stromal layer was slightly oedema, the conjunctival epithelial structure was incomplete, the arrangement was irregular, and the conjunctival epithelial cells proliferated. The basal layer cells were not arranged neatly, and inflammatory cell infiltration could be seen. Conclusion omega-3 can effectively inhibit tear secretion, enhance tear film stability and protect cornea and conjunctiva.
【作者單位】： 南方醫(yī)科大學(xué)珠江醫(yī)院眼科;
【基金】：國家自然科學(xué)基金面上項目(編號:81371067) 廣東省自然科學(xué)基金(編號:S2013040013713) 廣東省科技計劃項目(編號:201213061700106)~~
【分類號】：R777.34

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