本文選題：miRNA + 拷貝數(shù)變異��； 參考：《重慶醫(yī)科大學》2017年碩士論文

【摘要】：目的本研究旨在探討micro RNA(mi RNA)拷貝數(shù)變異與急性前葡萄膜炎伴或不伴發(fā)強直性脊柱炎之間的遺傳相關性以及其潛在的發(fā)病機制。方法本實驗共納入768例急性前葡萄膜炎伴或不伴發(fā)強直性脊柱炎的患者及660例正常人對照。我們采用Taq Man PCR法檢測mi RNA基因拷貝數(shù)分型,實時熒光定量PCR(Real-Time PCR)檢測mi RNA的m RNA表達水平,流式細胞術和ELISA法分別用來檢測mi R~(-9)-3的轉(zhuǎn)染效率以及細胞因子的表達水平。結果低拷貝(CNV2)的mi R-143、mi R-146a、mi R~(-9)-3、mi R-205以及高拷貝(CNV2)的mi R-301a和mi R-23a頻率在伴發(fā)強直性脊柱炎的急性前葡萄膜炎患者中明顯增高(P=3.725×10~(-5)至8.033×10~(-9))。我們還發(fā)現(xiàn),mi R-146a低拷貝頻率和mi R-23a、mi R-205的高拷貝頻率與不伴發(fā)強直性脊柱炎的急性前葡萄膜炎呈顯著性相關(P=0.002至P=0.001)。與正常人相比,mi R~(-9)-3,mi R-143和mi R-146a的m RNA表達水平在伴發(fā)強直性脊柱炎的急性前葡萄膜炎患者中顯著增高。另外,ELISA檢測發(fā)現(xiàn)IL-1β和IL-6在人視網(wǎng)膜色素上皮(h RPE)細胞中的表達量可能是由mi R~(-9)-3調(diào)控的。結論低拷貝的mi R-143、mi R-146a、mi R~(-9)-3和mi R-205以及高拷貝的mi R-301a和mi R-23a與伴發(fā)強直性脊柱炎的急性前葡萄膜炎遺傳易感性相關。低拷貝的mi R-146a和高拷貝的mi R-23a和mi R-205與不伴發(fā)強直性脊柱炎的急性前葡萄膜炎的發(fā)病呈相關性。
[Abstract]:Objective to investigate the genetic relationship between micro RNA(mi copy number variation and acute anterior uveitis with or without ankylosing spondylitis and its underlying pathogenesis. Methods 768 cases of acute anterior uveitis with or without ankylosing spondylitis and 660 normal controls were included. The Taq Man PCR method was used to detect the copy number typing of mi RNA gene, the m RNA expression level of mi RNA was detected by real-time fluorescence quantitative PCR(Real-Time PCR, and the transfection efficiency and cytokine expression level of mi RNA were detected by flow cytometry and ELISA, respectively. Results the frequency of mi R-301a and mi R-23a in low-copy CNV2) patients with acute anterior uveitis with ankylosing spondylitis was significantly higher than that in patients with acute anterior uveitis (P 3.725 脳 10 ~ (-5) to 8.033 脳 10 ~ (10). We also found that the low copy frequency of mi-mi R-146a and the high copy frequency of mi R-23aanmi R-205 were significantly correlated with acute anterior uveitis without ankylosing spondylitis. Compared with the normal controls, the expression levels of m RNA in the mitomyr-9ke-3a R-143 and mi R-146a were significantly higher in the patients with acute anterior uveitis associated with ankylosing spondylitis (ankylosing spondylitis). In addition, the expression of IL-1 尾 and IL-6 in human retinal pigment epithelium (RPE) cells was detected by Elisa. Conclusion the low copy of mi R-143Li mi R-146a mi r-146a mi r-9- 3 and mi R-205 and the high-copy miR-301a and miR-23a are associated with the genetic susceptibility of acute anterior uveitis with ankylosing spondylitis (ankylosing spondylitis). Low copy mi R-146a and high copy mi R-23a and mi R-205 were associated with acute anterior uveitis without ankylosing spondylitis.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R773.9

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