本文選題：脈絡(luò)膜新生血管 + Nd　； 參考：《眼科新進(jìn)展》2016年04期

【摘要】：目的評價采用不同功率的Nd:YAG激光(波長532 nm)誘導(dǎo)棕色挪威(Brown Norway,BN)大鼠脈絡(luò)膜新生血管(choroidal neovascularization,CNV)模型,為相關(guān)的藥物和器械評價提供適用的疾病動物模型。方法將96只BN大鼠隨機(jī)分為3個實驗組(分別為高、中、低功率組,激光功率依次為300 mW、200 mW、100 mW,光斑直徑100μm,曝光時間100 ms,每眼光凝8個點)和1個空白對照組,每組24只。各實驗組大鼠激光造模后的第1周、2周、3周、4周行眼底熒光血管造影(fundus fluorescein angiography,FFA),每個時間點各組隨機(jī)抽取2只大鼠行脈絡(luò)膜鋪片,隨機(jī)抽取4只大鼠雙眼行HE染色,以對比各組間CNV熒光素滲漏率、面積、厚度等差異。結(jié)果眼底彩照及FFA結(jié)果:中、高功率組第1周、2周、3周眼底熒光素滲漏率均高于低功率組(均為P0.01),中、高功率組差異均無統(tǒng)計學(xué)意義(均為P0.05),空白對照組晚期FFA圖像未見熒光素滲漏。脈絡(luò)膜鋪片結(jié)果:低功率組第1周、2周CNV面積變化不明顯,中、高功率組CNV面積從1周起隨時間增加而增大,各組CNV面積至第3周達(dá)到高峰,高功率組第4周的CNV面積仍大于低功率組(P0.05)。HE染色結(jié)果:各實驗組光凝后第1周,視網(wǎng)膜內(nèi)核層排列紊亂,大量增生的細(xì)胞穿破RPE層向視網(wǎng)膜內(nèi)層移行,形成初期CNV,隨時間推移CNV逐漸增厚,區(qū)域內(nèi)可見色素巨噬細(xì)胞、成纖維細(xì)胞,并夾雜新生血管,第4周區(qū)域周圍可見大量膠原纖維,各組CNV中央厚度變化與面積變化一致,均在第3周達(dá)到高峰,且第4周高功率組CNV中央厚度大于低功率組(P0.05),對照組視網(wǎng)膜內(nèi)核層細(xì)胞排列整齊,未見炎性反應(yīng)。結(jié)論 100 mW、200mW、300 m W的Nd:YAG激光均可以誘導(dǎo)BN大鼠CNV模型,其中300 m W的成模率最高,CNV面積及中央厚度最大,是Nd:YAG激光誘導(dǎo)BN大鼠CNV模型較為理想的激光功率。
[Abstract]:Objective to evaluate the choroidal neovascularization model induced by different power Nd:YAG laser (wavelength 532nm) in Brown NorwayBN rats, and to provide a suitable animal model for the evaluation of related drugs and instruments. Methods 96 BN rats were randomly divided into three experimental groups (high, middle and low power groups), with laser power of 300 MW / 200mW / W, light spot diameter 100 渭 m, exposure time 100msper eye, and a blank control group (24 rats in each group). Three weeks and four weeks after laser modeling, fundus fluorescein angiographic FFAA was performed in each experimental group. Two rats were randomly selected for choroidal preparation at each time point, and 4 rats were randomly selected for HE staining. The leakage rate, area and thickness of CNV fluorescein were compared. Results the fundus color radiography and FFA results showed that the fundus fluorescein leakage rate in the high power group was higher than that in the low power group (P 0.01, P 0.01, P < 0.05), and the fundus fluorescein leakage rate in the high power group was higher than that in the low power group. There was no significant difference between the high power group and the control group (P 0.05). No fluorescein leakage was found in the late FFA images of the blank control group. The results of choroidal film preparation showed that the area of CNV in the low power group did not change significantly at the 1st week and 2nd week. The area of CNV in the high power group increased with time from 1 week to the third week, and the area of CNV in each group reached its peak at the third week. The area of CNV in the high power group was still larger than that in the low power group at the 4th week. The results of HE staining were as follows: at the first week after photocoagulation, the nuclear layer of the retina was disarranged and a large number of proliferative cells broke through the RPE layer and migrated to the inner layer of the retina. In the early stage of CNV formation, CNV was gradually thickened with time. Pigment macrophages, fibroblasts and neovascularization were found in the region. A large number of collagen fibers were observed around the CNV area at the 4th week. The changes of central thickness of CNV were consistent with the area changes in each group. The central thickness of CNV in the high power group was larger than that in the low power group (P 0.05) at the 4th week, and the cells in the nuclear layer of the retina in the control group were arranged neatly and no inflammatory reaction was found. Conclusion the CNV model of BN rats can be induced by 100mW / 200mW / 300MW Nd:YAG laser, among which the mode-forming rate of 300MW is the highest and the central thickness is the largest, which is the ideal laser power for the CNV model of BN rats induced by Nd:YAG laser.
【作者單位】： 四川大學(xué)華西公共衛(wèi)生學(xué)院;四川大學(xué)華西醫(yī)院國家成都新藥安全性評價中心;
【基金】：科技部國家重大科學(xué)儀器設(shè)備開發(fā)專項(編號:2013YQ490859)~~
【分類號】：R-332;R773.4

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