本文選題：基質(zhì)細胞衍生因子-/基質(zhì)細胞衍生因子- + 大鼠　； 參考：《南方醫(yī)科大學(xué)學(xué)報》2016年12期

【摘要】：目的探討基質(zhì)細胞衍生因子-1(SDF-1)與基質(zhì)細胞衍生因子-1(CXCR4)在大鼠角膜組織中的表達及其在角膜移植術(shù)后免疫排斥反應(yīng)中的作用。方法取15只Wistar大鼠作為正常對照組;取22只Wistar大鼠行自體角膜移植術(shù)作為自體組;取22只SD大鼠與44只Wistar大鼠,以SD大鼠為供體,Wistar大鼠為受體行穿透性角膜移植,術(shù)后隨機抽取22只歸入典必殊組,術(shù)眼滴典必殊眼液(每日2次),剩余22只歸入異體組,術(shù)眼滴同等量生理鹽水,共一個月。參照Larkin法對各組角膜植片進行臨床評估;分別于術(shù)后第5、9天取術(shù)眼角膜植片,行組織病理學(xué)觀察、免疫組化檢查、實時熒光定量PCR檢測。結(jié)果自體組不發(fā)生排斥反應(yīng),典必殊組角膜存活時間為24±0.32 d,遠高于異體組10±0.36 d(P0.001)。組織病理學(xué)檢查發(fā)現(xiàn)異體組角膜有大量炎性細胞浸潤以及新生血管形成。SDF-1和CXCR4 mRNA在異體組角膜組織中表達水平明顯升高(第5天P0.001,第9天P0.01),典必殊組較異體組明顯降低。免疫組化檢查發(fā)現(xiàn)SDF-1/CXCR4主要表達在角膜植片的上皮層與基質(zhì)層,異體組角膜組織SDF-1和CXCR4含量明顯升高。結(jié)論 SDF-1/CXCR4可能參與了大鼠角膜移植術(shù)后早期的排斥反應(yīng),其機制可能為SDF-1特異性誘導(dǎo)CXCR4+細胞成熟和朝著排斥部位趨化,并促進角膜新生血管形成。
[Abstract]:Objective to investigate the expression of stromal cell derived factor -1 (SDF-1) and matrix cell derived factor -1 (CXCR4) in the corneal tissue of rats and its role in the immune rejection after corneal transplantation. Methods 15 rats were taken as the normal control group, and 22 Wistar rats were taken autologous corneal transplantation as an autologous group; 22 SD rats were obtained. 44 Wistar rats were treated with SD rats as donor, and Wistar rats were treated with penetrating keratoplasty. After operation, 22 were randomly selected from the group. The eye drops (2 times a day) and the remaining 22 were treated with the same amount of normal saline for one month. The corneal graft of each group was evaluated by Larkin method. Corneal graft was taken on day 5,9 after operation. Histopathological observation, immunohistochemical examination, real-time fluorescence quantitative PCR detection. The results showed no rejection in the autologous group. The corneal survival time of the group was 24 + 0.32 D, far higher than that of the allogeneic group, 10 + 0.36 D (P0.001). Histopathological examination found a large number of inflammatory cells infiltrated in the corneal allograft. The expression level of.SDF-1 and CXCR4 mRNA in the neovascularization was significantly increased in the corneal allograft tissue (fifth days P0.001, Ninth days P0.01), and the group of canbi group was significantly lower than that of the allogeneic group. The immunohistochemical examination showed that SDF-1/CXCR4 was mainly expressed in the epithelial layer and matrix layer of corneal graft, and the content of SDF-1 and CXCR4 in the corneal allograft was significantly increased. Conclusion SDF-1/CXCR4 may be involved in the early rejection after corneal transplantation in rats. The mechanism may induce the maturation of CXCR4+ cells and the chemotaxis toward the rejection site and promote the formation of corneal neovascularization by SDF-1 specificity.

【作者單位】： 南方醫(yī)科大學(xué)南方醫(yī)院眼科;南方醫(yī)科大學(xué)南方醫(yī)院惠僑醫(yī)療中心;廣東省婦幼保健院;
【基金】：廣東省自然科學(xué)基金(S2013010016640) 廣州市科技計劃項目(201607010386) 南方醫(yī)院院長基金(2014B014)
【分類號】：R779.6

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