本文選題：血管內(nèi)皮生長因子 + 缺氧誘導(dǎo)因子��； 參考：《青島大學(xué)》2017年碩士論文

【摘要】：目的:研究不同眼底表現(xiàn)的增生性糖尿病視網(wǎng)膜病變(proliferative diabetic retinopathy,PDR)患者玻璃體中血管內(nèi)皮生長因子(ascular endothelial growth factor,VEGF)、缺氧誘導(dǎo)因子-1α(hypoxia-inducible factor-1α,HIF-1α)、結(jié)締組織生長因子(connective tissue growth factor,CTGF)、轉(zhuǎn)化生長因子β2(transforming growth factorβ2,TGF-β2)的表達(dá)水平,探討此四種細(xì)胞因子在PDR發(fā)展中的差異表達(dá)。方法:選取68例因PDR而行玻璃體切除手術(shù)患者的玻璃體為實驗組(77眼),16例特發(fā)性黃斑裂孔(MH)患者玻璃體為對照組(16眼)。實驗組分為全視網(wǎng)膜光凝組(PRP組,12眼)和非全視網(wǎng)膜光凝組(NPRP組,65眼)。NPRP組包括視網(wǎng)膜脫離組(15眼)、萎縮膜組(19眼)、血管膜組(17眼)、玻璃體積血組(14眼)。用ELISA法對玻璃體標(biāo)本進(jìn)行VEGF、HIF-1α、CTGF、TGF-β2定量檢測,并對比分析各組間差別。結(jié)果:1.PDR患者玻璃體VEGF、HIF-1α、CTGF、TGF-β2濃度分別為(3064.54±955.41)pg/ml,(0.672±0.204)ng/ml,(6.144±1.382)ng/ml,(3416.45±95.31)pg/ml。MH組玻璃體VEGF、HIF-1α、CTGF、TGF-β2濃度分別為(175.98±49.11)pg/ml,(0.331±0.028)ng/ml,(3.246±0.513)ng/ml,(713.64±182.45)pg/ml。PDR組玻璃體四種細(xì)胞因子含量均高于對照組,差異均有統(tǒng)計學(xué)意義(P0.05)。2.PRP患者玻璃體VEGF、HIF-1α、CTGF、TGF-β2濃度分別為(1035.76±438.54)pg/ml,(0.420±0.197)ng/ml,(4.109±0.774)ng/ml,(1049.09±158.53)pg/ml。NPRP組玻璃體VEGF、HIF-1α、CTGF、TGF-β2濃度分別為(2157.59±1002.84)pg/ml,(0.578±0.203)ng/ml,(6.611±0.034)ng/ml,(2227.18±962.61)pg/ml。PRP組玻璃體四種細(xì)胞因子含量均低于NPRP組,差異均有統(tǒng)計學(xué)意義(P0.05)。3.VEGF:視網(wǎng)膜脫離組、萎縮膜組、血管膜組、玻璃體積血組中VEGF表達(dá)量分別為(2946.93±1097.16)pg/ml、(1915.74±985.04)pg/ml、(1897.80±842.71)pg/ml、(1573.62±606.33)pg/ml。四組玻璃體中VEGF表達(dá)量相比較,視網(wǎng)膜脫離組萎縮膜組=血管膜組玻璃體積血組,除萎縮膜組與血管膜組相比較,差異無統(tǒng)計學(xué)意義(P0.05)外,其余各組間相比較,差異均有統(tǒng)計學(xué)意義(P0.05)。4.HIF-1α:視網(wǎng)膜脫離組、萎縮膜組、血管膜組、玻璃體積血組中HIF-1α表達(dá)量分別為(0.637±0.195)ng/ml、(0.551±0.210)ng/ml、(0.549±0.187)ng/ml、(0.472±0.113)ng/ml。四組玻璃體中HIF-1α表達(dá)量相比較,視網(wǎng)膜脫離組萎縮膜組=血管膜組玻璃體積血組,除萎縮膜組與血管膜組相比較,差異無統(tǒng)計學(xué)意義(P0.05)外,其余各組間相比較,差異均有統(tǒng)計學(xué)意義(P0.05)。5.CTGF:視網(wǎng)膜脫離組、萎縮膜組、血管膜組、玻璃體積血組中CTGF表達(dá)量分別為(6.420±0.165)ng/ml、(5.767±0.124)ng/ml、(5.131±0.810)ng/ml、(4.507±0.712)ng/ml。四組玻璃體中CTGF表達(dá)量相比較,視網(wǎng)膜脫離組萎縮膜組血管膜組玻璃體積血組,各組間相比較,差異均有統(tǒng)計學(xué)意義(P0.05)。6.TGF-β2:視網(wǎng)膜脫離組、萎縮膜組、血管膜組、玻璃體積血組中TGF-β2表達(dá)量分別為(3105.29±931.71)pg/ml、(2658.55±873.01)pg/ml、(1918.26±114.58)pg/ml、(1327.18±602.63)pg/ml。四組玻璃體中TGF-β2表達(dá)量相比較,視網(wǎng)膜脫離組萎縮膜組血管膜組玻璃體積血組,各組間相比較,差異均有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:DR發(fā)展的不同時期,玻璃體內(nèi)VEGF、HIF-1α、CTGF、TGF-β2表達(dá)有差異,玻璃體中四種細(xì)胞因子表達(dá)與PDR嚴(yán)重程度有關(guān)。
[Abstract]:Objective: To study the vascular endothelial growth factor (ascular endothelial growth factor, VEGF) in the vitreous body of proliferative diabetic retinopathy (PDR) with different fundus manifestations and the hypoxic inducible factor -1 alpha (hypoxia-inducible factor-1 alpha) and connective tissue growth factor (connective tissue growth factor). H factor, CTGF), the expression level of transforming growth factor beta 2 (transforming growth factor beta 2, TGF- beta 2), and to explore the differential expression of the four cytokines in PDR development. Methods: the vitreous body of 68 cases with vitrectomy with PDR was selected as the experimental group (77 eyes), and 16 cases of idiopathic macular hole (MH) patients with vitreous body (16) were used as the control group (16 The experimental group was divided into total retinal photocoagulation group (group PRP, 12 eyes) and non full retinal photocoagulation group (group NPRP, 65 eyes).NPRP group including retinal detachment group (15 eyes), atrophic membrane group (19 eyes), vascular membrane group (17 eyes), vitreous blood group (14 eyes). ELISA method was used to detect VEGF, HIF-1 alpha, CTGF, TGF- beta 2 in vitreous specimens, and compared and analyzed each group. Results: the concentrations of VEGF, HIF-1, CTGF and TGF- beta 2 in the vitreous body of 1.PDR patients were (3064.54 + 955.41) pg/ml, (0.672 + 0.204) ng/ml, (6.144 + 1.382) ng/ml, (3416.45 + 95.31) pg/ml.MH group vitreous VEGF, HIF-1 alpha, and 2 concentration respectively (175.98 + 49.11), respectively. The contents of four kinds of cytokines in the vitreous body of group PDR were higher than those in the control group. The differences were statistically significant (P0.05) in the vitreous VEGF, HIF-1 a, CTGF, TGF- beta 2, respectively (1035.76 + 438.54) pg/ml, (0.420 + 0.197) ng/ml, (4.109 + 0.774) ng/ml, (1049.09 + 158.53) pg/ml.NPRP group vitreous VEGF, 2 concentration (215), respectively (215) 7.59 + 1002.84) pg/ml, (0.578 + 0.203) ng/ml, (6.611 + 0.034) ng/ml, (2227.18 + 962.61) pg/ml.PRP group of vitreous body four kinds of cytokines were lower than the NPRP group, the difference was statistically significant (P0.05).3.VEGF: retinal detachment group, atrophic membrane group, vascular membrane group, vitreous blood group VEGF expression of (2946.93 + 1097.16) pg/ml, respectively (1915.). 74 + 985.04) pg/ml, (1897.80 + 842.71) pg/ml, (1573.62 + 606.33) pg/ml. four vitreous body VEGF expression phase comparison, retinal detachment group atrophy membrane group = vitreous blood group of vascular membrane group, except for the atrophy membrane group and the vascular group, the difference was not statistically significant (P0.05), the other groups were compared, the difference was statistically significant (P0.05 .4.HIF-1 alpha: the expression of HIF-1 alpha in the retinal detachment group, the atrophy membrane group, the vascular membrane group and the vitreous blood group were (0.637 + 0.195) ng/ml, (0.551 + 0.210) ng/ml, (0.549 + 0.187) ng/ml, and (0.472 + 0.113) ng/ml. four groups in the vitreous body, compared with the atrophy of the retinal detachment group = the vitreous blood group of the vascular membrane group, except for atrophy. The difference of the membrane group and the vascular group was not statistically significant (P0.05), and the differences were statistically significant (P0.05).5.CTGF: retinal detachment group, atrophic membrane group, vascular membrane group, and vitreous blood group, CTGF expression was (6.420 + 0.165) ng/ml, (5.767 + 0.124) ng/ml, (5.131 + 0.810) ng/ml, (4.507 + 0.712) ng/m The expression of CTGF in the vitreous body of L. four groups was compared. The difference in the vitreous blood group of the retinal detachment group was statistically significant (P0.05) in the.6.TGF- beta 2: retinal detachment group, the expression of TGF- beta 2 in the atrophic membrane group, the vascular membrane group and the vitreous blood group was (3105.29 + 931.71) pg/ml, respectively, (2658.55 + 873.01). Pg/ml, (1918.26 + 114.58) pg/ml, (1327.18 + 602.63) pg/ml. four groups of vitreous TGF- beta 2 expression phase comparison, retinal detachment group atrophy membrane group Glass Group vitreous blood group, the difference is statistically significant (P0.05). Conclusion: there are differences in the expression of VEGF, HIF-1 a, CTGF, TGF- beta 2 in the different stages of the development of DR. The expression of four cytokines in vitreous body is related to the severity of PDR.

【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R587.2;R774.1

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