發(fā)布時間：2018-04-27 03:17

  本文選題：DMBT1 + 鼻息肉　； 參考：《第二軍醫(yī)大學(xué)》2017年碩士論文

【摘要】：目的探討DMBT1基因在鼻息肉發(fā)生發(fā)展中的作用及其機制。方法用shRNA敲降法建立體外穩(wěn)定低表達DMBT1的鼻黏膜上皮細胞系,實驗分三組:實驗組(shRNA敲降DMBT1的鼻黏膜上皮細胞),對照組(未做任何處理的鼻黏膜上皮細胞),空載體組(轉(zhuǎn)染空載體的鼻黏膜上皮細胞),CCK8法及流式細胞術(shù)檢測各組的鼻黏膜上皮細胞的增殖、凋亡及細胞周期變化,western-blot檢測各組的鼻黏膜上皮細胞中AKT、P53、STAT3及ERK1/2表達變化,并觀察DMBT1作用的信號通路。結(jié)果實驗組與對照組比較:鼻黏膜上皮細胞增殖增加,凋亡減少,細胞周期中G1期細胞明顯減少,G2和S期細胞明顯增多;AKT明顯上調(diào)(P㩳0.05),P53蛋白明顯下調(diào)(P㩳0.05);ERK1/2、STAT3的表達雖有微小差異,但差異無統(tǒng)計學(xué)意義(P㧐0.05)。對照組與空載體組比較:細胞增殖凋亡及細胞周期改變和AKT、P53、ERK1/2、STAT3差異均無統(tǒng)計學(xué)意義(P㧐0.05)。結(jié)論DMBT1基因在鼻息肉發(fā)生發(fā)展中起重要作用,其機制可能為:DMBT1表達下調(diào)通過“AKT-P53”信號通路促使鼻黏膜上皮細胞增殖增加、凋亡減少,促進鼻息肉產(chǎn)生。
[Abstract]:Objective to investigate the role and mechanism of DMBT1 gene in the development of nasal polyps. Methods nasal mucosal epithelial cell lines with stable and low expression of DMBT1 were established by shRNA knockdown in vitro. The experiment was divided into three groups: the experimental group was used to knock down the nasal mucosal epithelial cells of DMBT1, the control group was used to detect the nasal mucosal epithelial cells without any treatment, and the empty vector group (the empty vector transfected nasal mucosal epithelial cells was detected by CCK8 method and flow cytometry). The proliferation of nasal mucosal epithelial cells, Apoptosis and cell cycle changes were detected by Western blot in nasal epithelial cells of each group. The expression of AKTP-53-STAT3 and ERK1/2 were detected and the signal pathway induced by DMBT1 was observed. Results compared with the control group, the proliferation and apoptosis of nasal epithelial cells in the experimental group were increased, and the number of cells in G _ 1 phase and S phase in the cell cycle were significantly decreased. AKT significantly up-regulated the expression of P0. 05 and P0. 05 and ERK1 / 2 + STAT3, although there was a slight difference. But the difference was not statistically significant. There was no significant difference between control group and empty carrier group in cell proliferation, apoptosis, cell cycle change and AKT53ERK1 / 2STAT3. Conclusion DMBT1 gene plays an important role in the occurrence and development of nasal polyps, and its mechanism may be that the down-regulation of the expression of DMBT1 may promote the proliferation of nasal epithelial cells and decrease of apoptosis through the "AKT-P53" signaling pathway, thus promoting the production of nasal polyps.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R765

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