本文選題：視神經(jīng)損傷 + 視網(wǎng)膜神經(jīng)節(jié)細(xì)胞�。� 參考：《重慶醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的探討超聲微泡造影劑聯(lián)合美金胺(memantine),對由視神經(jīng)鉗夾造成損傷的大鼠視網(wǎng)膜神經(jīng)節(jié)細(xì)胞（RGC）的保護(hù)作用。 方法將40只SD雄性成年大鼠按隨機(jī)分組法分為以下5個處理組: A組：正常對照組，B組：假手術(shù)組，C組：空白對照組，D組：玻璃體腔單獨注射美金胺組，E組：玻璃體腔注射美金胺加超聲微泡組，每組大鼠的數(shù)量均為8只，各組再按觀察時間點的不同，隨機(jī)分為:視神經(jīng)損傷后第1周和第2周2個亞組，各亞組的大鼠數(shù)量均為4只。A組不做任何處理；B組只暴露視神經(jīng)，不進(jìn)行鉗夾，玻璃體腔注射生理鹽水，立即用超聲波輻照大鼠眼球；C～E組建立視神經(jīng)鉗夾傷模型后，處理方式分別為C組玻璃體腔注射生理鹽水，D組玻璃體腔注射美金胺，E組玻璃體腔注射超聲微泡造影劑，再以相同方法玻璃體腔注射美金胺，之后立即用超聲波輻照大鼠眼球。視神經(jīng)鉗夾后第1、第2周時，A～E組分別用熒光金（FG）逆行標(biāo)記RGC并計數(shù)；閃光視覺誘發(fā)電位（F-VEP）檢測，記錄P_(100)波潛伏期及振幅；熒光電子顯微鏡下觀察視網(wǎng)膜細(xì)胞形態(tài)學(xué)改變。 結(jié)果逆行熒光金(FG)標(biāo)記RGC結(jié)果顯示，各處理組視網(wǎng)膜定向鋪片上均可見金黃色著染的RGC。A、B組間RGC數(shù)差異沒有統(tǒng)計學(xué)意義(q=0.018，，0.011；P=0.986，0.873)；C～E組RGC數(shù)均較A組減少，差異具有統(tǒng)計學(xué)意義（F=85.944，P=0.012）；D組RGC數(shù)多于C組，差異具有統(tǒng)計學(xué)意義（q=1.721,1.924；P=0.043,0.037）；E組RGC數(shù)明顯高于C、D組，差異具有統(tǒng)計學(xué)意義（q=1.128,1.482，P=0.027,0.008；q=1.453,1.855，P=0.031,0.010）。F-VEP檢測發(fā)現(xiàn)，A、B組間P100波潛伏期及振幅差異沒有統(tǒng)計學(xué)意義（q=0.008，0.019，P=0.981,0.946；q=0.072,0.052，P=0.737,0.851）；C～E組P100波潛伏期較A組延長，振幅較A組降低，差異具有統(tǒng)計學(xué)意義（F=134.312，F(xiàn)=106.312，P=0.017,0.009）。熒光電子顯微鏡下觀察發(fā)現(xiàn)，A、B組大鼠視網(wǎng)膜各層結(jié)構(gòu)完整，排列整齊，RGC排列緊密整齊，細(xì)胞核均勻深染，胞核大小一致。C～E組大鼠的視網(wǎng)膜不同程度的水腫變厚，RGC有不同程度的排列紊亂，空泡化及細(xì)胞數(shù)目的減少。 結(jié)論超聲微泡造影劑聯(lián)合美金胺能抑制視神經(jīng)損傷后大鼠RGC的丟失，促進(jìn)其視功能的恢復(fù)，對視神經(jīng)損傷大鼠的RGC具有保護(hù)作用。
[Abstract]:Objective to investigate the protective effect of ultrasound microbubble contrast agent combined with medeline memantine on retinal ganglion cells (RGC) injured by optic forceps in rats. Methods Forty Sprague-Dawley male adult rats were randomly divided into the following five groups: group A: normal control group: group B: sham operation group: group C: blank control group: group D: intravitreous injection of mebamine alone: group E; Vitreous injection of mebamine plus ultrasound microbubble group, The number of rats in each group was 8. Each group was randomly divided into two subgroups according to the observed time points: the first week and the second week after optic nerve injury. The number of rats in each subgroup was 4 rats in group A and only exposed to optic nerve in group B without any treatment. Without clamp, normal saline was injected into the vitreous cavity, and the model of optic nerve clamp injury was established in group C (C) E by ultrasonic irradiation. The methods of treatment were as follows: group C was injected with normal saline and group D was injected with menamine E group by intravitreal injection of ultrasound microbubble contrast agent, and then intravitreous injection of mebamine was used in the same way, and then the eyeball of rats was irradiated with ultrasound immediately. In the first and second week after optic forceps clamp, the RGC was labeled with FG retrograde and counted, the latency and amplitude of the wave were recorded by flash visual evoked potential (F-VEP), and the morphological changes of retinal cells were observed under fluorescence electron microscope. Results the results of RGC labeled with retrograde FG showed that the RGC number of group B with golden yellow staining was not significantly different from that of group A (P 0.9860.873C), and the number of RGC in group E was lower than that in group A. The number of RGC in group D was significantly higher than that in group C, and the number of RGC in group E was significantly higher than that in group C. The results of fluorescence electron microscopy showed that all layers of retina in group A were intact, and RGC was arranged closely, and the nucleus was homogeneous and deep stained. The retinal edema and thickening of RGC of different degrees in the same size of nucleus. The RGC had different degree of arrangement disorder, vacuolation and the decrease of the number of cells. Conclusion Ultrasonic microbubble contrast agent combined with mebamine can inhibit the loss of RGC and promote the recovery of visual function in rats with optic nerve injury. It has a protective effect on RGC in rats with optic nerve injury.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R774.1

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