本文選題：細(xì)胞黏附分子 + CD44v5基因　； 參考：《遼寧醫(yī)學(xué)院》2012年碩士論文

【摘要】：目的 喉癌惡性程度較高，易發(fā)生淋巴轉(zhuǎn)移。惡性腫瘤的發(fā)展進(jìn)程是一個(gè)復(fù)雜連續(xù)的過(guò)程，在此過(guò)程中，腫瘤細(xì)胞與血管及淋巴管基底膜的黏附非常重要。細(xì)胞黏附分子CD44v5、CD44v6與許多惡性腫瘤的浸潤(rùn)、轉(zhuǎn)移密切相關(guān)，并直接影響患者的預(yù)后。本研究通過(guò)觀察喉癌及癌旁組織中細(xì)胞黏附分子CD44v5、CD44v6的表達(dá)變化情況來(lái)探討其臨床意義。 方法 采用免疫組織化學(xué)SP法檢測(cè)78例喉癌組織及14例癌旁正常組織中CD44v5、 CD44v6表達(dá)變化。 RT-PCR法和Western蛋白印跡法鑒定CD44v5、CD44v6的表達(dá)情況。采用SPSS13.0統(tǒng)計(jì)分析軟件處理實(shí)驗(yàn)數(shù)據(jù)，X~2檢驗(yàn)、t檢驗(yàn)進(jìn)行分析。 結(jié)果 CD44v5在喉癌組織和癌旁組織中均有表達(dá)，，以喉癌組織中的陽(yáng)性表達(dá)占優(yōu)勢(shì)，兩組比較，P＜0.05。CD44v5在喉癌中陽(yáng)性表達(dá)率Ⅰ～Ⅱ期明顯低于Ⅲ～Ⅳ期，無(wú)淋巴結(jié)轉(zhuǎn)移者明顯低于有淋巴結(jié)轉(zhuǎn)移者，且隨喉癌組織學(xué)分化程度的升高而降低。 CD44v6僅在喉癌組織中有表達(dá)，癌旁組織中無(wú)表達(dá)，兩組比較，P＜0.05。CD44v6在喉癌中陽(yáng)性表達(dá)率Ⅰ～Ⅱ期明顯低于Ⅲ～Ⅳ期，無(wú)淋巴結(jié)轉(zhuǎn)移者明顯低于有淋巴結(jié)轉(zhuǎn)移者，且呈隨喉癌組織學(xué)分化程度的升高而降低的趨勢(shì)。 結(jié)論 CD44v5、CD44v6在喉癌組織中均表達(dá)，其中CD44v6表達(dá)特異性更高一些。 CD44v5、CD44v6的表達(dá)，與喉癌的TNM分期及有無(wú)淋巴結(jié)轉(zhuǎn)移呈正相關(guān)，與組織分化程度呈負(fù)相關(guān)。CD44v5、CD44v6在喉癌中的高表達(dá)與腫瘤的侵襲、淋巴結(jié)的轉(zhuǎn)移密切相關(guān)，且CD44v6與細(xì)胞的分化程度密切相關(guān)。
[Abstract]:Purpose Laryngeal carcinoma has a high degree of malignancy and is prone to lymphatic metastasis. The development of malignant tumors is a complex and continuous process in which the adhesion of tumor cells to blood vessels and lymphatic basement membrane is very important. The cell adhesion molecule CD44v5, CD44v6 is closely related to the invasion and metastasis of many malignant tumors, and directly affects the prognosis of the patients. The aim of this study was to investigate the clinical significance of CD44v5 and CD44v6 expression in laryngeal carcinoma and paracancerous tissues. Method Immunohistochemical SP method was used to detect the expression of CD44v5 and CD44v6 in 78 cases of laryngeal carcinoma and 14 cases of adjacent normal tissues. The expression of CD44v5, CD44v6 was identified by RT-PCR and Western Western blot. The SPSS13.0 statistical analysis software was used to process the experimental data. Result The expression of CD44v5 was found in laryngeal carcinoma and paracancerous tissues. The positive expression rate of 0.05.CD44v5 in laryngeal carcinoma was significantly lower in stage 鈪

本文編號(hào)：1784845