發(fā)布時(shí)間：2018-04-20 13:52

  本文選題：單純皰疹病毒型 + 樹(shù)突狀細(xì)胞靶向DNA疫苗�。� 參考：《現(xiàn)代醫(yī)學(xué)》2016年11期

【摘要】：目的:構(gòu)建靶向樹(shù)突狀細(xì)胞(DC)的DNA疫苗pRSC-NLDC145·gD-IL-21,針對(duì)抵抗鼠眼角膜單純皰疹病毒1型(HSV-1)的感染,探討其是否能產(chǎn)生更強(qiáng)的免疫應(yīng)答反應(yīng)。方法:通過(guò)基因克隆的方法構(gòu)建靶向DC的重組質(zhì)粒pRSC-NLDC145·gD-IL-21,對(duì)其進(jìn)行鑒定,并和殼聚糖包裹組成納米DNA疫苗,分別以靶向組pRSC-NLDC145·gD-IL-21+殼聚糖、非靶向組pRSC-gD-IL-21+殼聚糖、pRSC+殼聚糖和殼聚糖免疫小鼠3次,間隔2周,檢測(cè)各疫苗對(duì)小鼠免疫應(yīng)答的水平。結(jié)果:靶向DC的重組質(zhì)�？稍谡婧思�(xì)胞內(nèi)進(jìn)行蛋白水平的表達(dá),且和納米殼聚糖包裹形成包封率較高的靶向DNA疫苗組,較非靶向組在鼠體內(nèi)產(chǎn)生了更強(qiáng)的免疫應(yīng)答反應(yīng)。結(jié)論:眼表黏膜接種靶向DC的納米DNA疫苗pRSC-NLDC145·gD-IL-21,在小鼠單純皰疹病毒性角膜炎(HSK)動(dòng)物模型中顯示了更強(qiáng)的免疫保護(hù)反應(yīng)。
[Abstract]:Aim: to construct a DNA vaccine pRSC-NLDC145 gD-IL-21 targeting dendritic cells (DC) and investigate whether it can produce a stronger immune response to the infection of corneal herpes simplex virus type 1 (HSV-1) in mice. Methods: the recombinant plasmid pRSC-NLDC145 gD-IL-21 was constructed by gene cloning. The recombinant plasmid pRSC-NLDC145 gD-IL-21 was identified and encapsulated with chitosan to form a DNA vaccine. PRSC-NLDC145 gD-IL-21 chitosan was used as the target group. Mice were immunized with pRSC-gD-IL-21 chitosan and chitosan for 3 times, at intervals of 2 weeks, to detect the level of immune response of each vaccine to mice. Results: the recombinant plasmid targeting DC could express protein level in eukaryotic cells, and encapsulated with nano-chitosan to form a higher encapsulation efficiency of targeted DNA vaccine group, which had a stronger immune response than non-target group in mice. Conclusion: pRSC-NLDC145 gD-IL-21, a nano-sized DNA vaccine targeting DC, showed a stronger immune protective response in mice with herpes simplex keratitis.
【作者單位】： 東南大學(xué)醫(yī)學(xué)院眼科學(xué)系;南京大學(xué)醫(yī)學(xué)院附屬鼓樓醫(yī)院眼科;
【分類(lèi)號(hào)】：R772.21
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本文編號(hào)：1778043