本文選題：視網(wǎng)膜新生血管 + 奧曲肽��； 參考：《山西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：研究奧曲肽玻璃體腔注射對(duì)高氧誘導(dǎo)小鼠視網(wǎng)膜新生血管模型的抑制作用及其機(jī)制探討。 方法：將健康的鼠齡7d天的昆明小鼠24只隨機(jī)分為2組，正常組和高氧組，正常組8只，高氧組16只，正常組（A組）于正�？諝猸h(huán)境中飼養(yǎng)5天，高氧組置于密閉的自制氧箱內(nèi)飼養(yǎng)5天，高氧組又分為實(shí)驗(yàn)組（B組）和對(duì)照組（C組），每組各8只，鼠齡12天時(shí)，B組玻璃體腔內(nèi)注射奧曲肽3μl，A組和C組玻璃體腔內(nèi)注射等量生理鹽水，給藥后第7天，鼠齡19天時(shí)將其處死，摘除眼球，分別行視網(wǎng)膜鋪片光鏡下觀察視網(wǎng)膜血管形態(tài)；HE染色計(jì)數(shù)突破視網(wǎng)膜內(nèi)界膜的內(nèi)皮細(xì)胞核平均數(shù)；免疫組化定量分析VEGF和IGF-1在視網(wǎng)膜內(nèi)的表達(dá)。統(tǒng)計(jì)結(jié)果以均數(shù)士標(biāo)準(zhǔn)差表示，應(yīng)用SPSS13.00軟件進(jìn)行統(tǒng)計(jì)分析，采用方差分析，組間兩樣本比較采用LSD-t檢驗(yàn)，P＜0.05認(rèn)為差異有統(tǒng)計(jì)學(xué)意義。 結(jié)果：視網(wǎng)膜鋪片A組視網(wǎng)膜血管均勻分布，管徑較粗，分支良好，周邊視網(wǎng)膜血管網(wǎng)結(jié)構(gòu)清晰。B組視網(wǎng)膜血管分布不均勻，走行部分僵直，管徑部分變細(xì)，視盤周圍毛細(xì)血管部分閉塞，偶見無灌注區(qū)，周邊視網(wǎng)膜分支小血管狹窄，無閉塞。C組視網(wǎng)膜血管分布不均勻，走行僵直，管徑變細(xì)，視盤周圍毛細(xì)血管閉塞，，見大片無灌注區(qū)，周邊視網(wǎng)膜分支小血管狹窄，甚至閉塞，可見無灌注區(qū)。A、B、C組HE染色突破視網(wǎng)膜內(nèi)界膜的血管內(nèi)皮細(xì)胞核平均數(shù)呈增加趨勢，分別為（0.27±0.23）、（1.77±0.38）及（3.57±0.71）個(gè)/HP，各組間差異顯著（P＜0.05)，A、B、C組視網(wǎng)膜內(nèi)VEGF的陽性表達(dá)有增強(qiáng)趨勢，其平均灰度值測量依次為（160.82±5.90）、（127.14±4.40）、（104.22±3.18），各組間差異顯著（P＜0.05)，A、B、C組視網(wǎng)膜內(nèi)IGF-1的陽性表達(dá)亦有增強(qiáng)趨勢，其平均灰度值測量依次為（145.23±5.58）、（126.65±5.13）、（105.14±4.49），同時(shí)視網(wǎng)膜內(nèi)VEGF與IGF-1的表達(dá)，運(yùn)用Spearman等級(jí)相關(guān)檢驗(yàn)顯示，二者有正相關(guān)性(P0.01)。 結(jié)論：奧曲肽能有效抑制小鼠視網(wǎng)膜新生血管的形成，同時(shí)降低VEGF和IGF-1在視網(wǎng)膜內(nèi)的表達(dá)水平。
[Abstract]:Aim: to study the inhibitory effect of octreotide intravitreal injection on hyperoxia-induced retinal neovascularization in mice and its mechanism.Methods: Twenty-four Kunming mice aged 7 days were randomly divided into two groups: normal group and hyperoxia group, 8 normal mice, 16 hyperoxia group, and normal group A) were fed in normal air environment for 5 days.The hyperoxia group was kept in an airtight self-made oxygen box for 5 days. The hyperoxia group was divided into two groups: the experimental group (group B) and the control group (group C, 8 rats in each group).At 12 days of age, octreotide 3 渭 l of octreotide was injected into the vitreous cavity of rats in group B and group C was injected with the same amount of normal saline into the vitreous cavity. On the 7th day after administration, the rats were killed at 19 days of age and their eyeballs were removed.The average number of endothelial nuclei that broke through the retinal inner boundary membrane was counted by HE staining and the expression of VEGF and IGF-1 in the retina were quantitatively analyzed by immunohistochemistry.The statistical results were expressed as the standard deviation of mean value. The statistical analysis was carried out by using SPSS13.00 software. The analysis of variance was used. The comparison between the two samples was compared by LSD-t test (P < 0. 05) and the difference was statistically significant (P < 0. 05).Results: in group A, the retinal vessels were evenly distributed, the diameter of retinal vessels was coarse, the branches were good, the structure of peripheral retinal vascular network was clear and the distribution of retinal vessels in group B was not uniform.The peripheral capillaries were partially occluded, there was no perfusion area, the peripheral retinal branches were narrow, the retinal vessels in group C were not evenly distributed, the retinal vessels were stiffened, the diameter of the vessels became thinner, and the capillaries were occluded around the optic disc.A large area without perfusion was seen, and the peripheral retinal branches were narrow or even occluded. It was found that the number of endothelial nuclei in the non-perfused area.The positive expression of VEGF in retina of group A (0.27 鹵0.23) was 1.77 鹵0.38 (P < 0.05) and that of group C (3.57 鹵0.71) was 3.57 鹵0.71). The average gray value was 160.82 鹵5.90 (127.14 鹵4.404.40 鹵104.22 鹵3.18), and the positive expression of IGF-1 in retina was also increased in group C (P < 0.05).The average grayscale value was 145.23 鹵5.58, 126.65 鹵5.13, 105.14 鹵4.49, and the expression of VEGF and IGF-1 in retina was positively correlated with Spearman grade correlation test (P0.01).Conclusion: octreotide can effectively inhibit the formation of retinal neovascularization and decrease the expression of VEGF and IGF-1 in the retina.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R774.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前3條

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相關(guān)博士學(xué)位論文 前3條

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相關(guān)碩士學(xué)位論文 前10條

1 王家玲;消朦靈對(duì)糖尿病大鼠視網(wǎng)膜TM-1、JAK/STAT通路的調(diào)控作用[D];廣州中醫(yī)藥大學(xué);2011年

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10 李靈;比較Avastin兩種不同用藥方法抑制角膜堿燒傷后早期新生血管增生的實(shí)驗(yàn)研究[D];中南大學(xué);2008年

本文編號(hào)：1763836