本文選題：喉癌和下咽癌 + PHF8　； 參考：《中南大學(xué)》2012年碩士論文

【摘要】：目的：探討組蛋白去甲基化酶PHD鋅指蛋白8(PHF8)蛋白質(zhì)在喉癌,下咽癌組織及癌旁組織中的表達(dá),同時(shí)探討其表達(dá)與喉癌,下咽癌臨床病理特征和預(yù)后之間的關(guān)系。 方法：采用免疫組織化學(xué)方法檢測(cè)PHF8蛋白在95例喉癌,下咽癌中的表達(dá)情況,并統(tǒng)計(jì)分析PHF8蛋白與喉癌,下咽癌的臨床病理特征及預(yù)后之間的關(guān)系；我們運(yùn)用Western blot方法檢測(cè)12例配對(duì)的喉癌,下咽癌及癌旁組織中的PHF8蛋白表達(dá)水平的差異。 結(jié)果：PHF8蛋白在喉癌,下咽癌組織中表達(dá)上調(diào)。PHF8蛋白的表達(dá)與喉癌,下咽癌的腫瘤發(fā)生的位置(P=0.001)、T分級(jí)(P=0.008)、臨床分期(P=0.039)、復(fù)發(fā)(P=0.023)及預(yù)后密切相關(guān)；Kaplan-Meier生存分析結(jié)果顯示PHF8高表達(dá)組與低表達(dá)組比較,高表達(dá)組無瘤生存率低于低表達(dá)組且高表達(dá)組的整體生存率低于低表達(dá)組,多因素Cox比例風(fēng)險(xiǎn)回歸模型分析進(jìn)一步顯示,PHF8蛋白表達(dá)水平可作為喉癌,下咽癌預(yù)后的獨(dú)立影響因素(P=0.037)。 結(jié)論：PHF8在喉癌,下咽癌組織中的表達(dá)上調(diào),PHF8在喉癌,下咽癌患者高表達(dá)與腫瘤的位置、T分級(jí)、復(fù)發(fā)、臨床分期、及患者的預(yù)后相關(guān)。結(jié)果顯示PHF8在喉癌,下咽癌中的發(fā)生、發(fā)展中可能發(fā)揮了重要的作用,PHF8可能成為預(yù)測(cè)喉癌,下咽癌術(shù)后復(fù)發(fā)及預(yù)后的重要標(biāo)志。
[Abstract]:Objective : To investigate the expression of the protein 8 ( PHF8 ) protein in laryngeal carcinoma , hypopharyngeal carcinoma and adjacent tissues , and to explore the relationship between its expression and the clinicopathological features and prognosis of laryngeal carcinoma and hypopharyngeal carcinoma .

Methods : The expression of PHF8 protein in 95 patients with laryngeal carcinoma and hypopharyngeal carcinoma was detected by immunohistochemistry . The relationship between PHF8 protein and the clinicopathological features and prognosis of laryngeal carcinoma and hypopharyngeal carcinoma was analyzed .
Western blot was used to detect the expression of PHF8 protein in 12 paired laryngeal carcinoma , hypopharyngeal carcinoma and paracancer tissues .

Results : The expression of PHF8 protein was up - regulated in laryngeal carcinoma and hypopharyngeal carcinoma . The expression of PHF8 protein was closely related to the location of laryngeal carcinoma and hypopharyngeal carcinoma ( P = 0.001 ) , T grade ( P = 0.008 ) , clinical stage ( P = 0.039 ) , recurrence ( P = 0.023 ) and prognosis .
The Kaplan - Meier survival analysis showed that the high expression level of PHF8 was lower than that in low expression group , and the overall survival rate of high expression group was lower than that in low expression group . Multivariate Cox proportional hazard regression model analysis showed that PHF8 protein expression level could be used as an independent prognostic factor for laryngeal cancer and hypopharyngeal carcinoma ( P = 0.037 ) .

Conclusion : The expression of PHF8 is up - regulated in laryngeal carcinoma and hypopharyngeal carcinoma . The expression of PHF8 is related to the location , T grade , recurrence , clinical stage and prognosis of patients with laryngeal carcinoma and hypopharyngeal carcinoma . The results show that PHF8 may play an important role in the development of laryngeal carcinoma and hypopharyngeal carcinoma .

【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R739.6

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 陳飛;李彬;王力紅;梁傳余;劉世喜;葉惠平;;喉及下咽部鱗癌頸淋巴結(jié)轉(zhuǎn)移的相關(guān)研究[J];西部醫(yī)學(xué);2007年05期

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