本文選題：硫酸慶大霉素　切入點(diǎn)：耳蝸　出處：《遼寧醫(yī)學(xué)院》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 觀察腹腔注射氨基糖苷類抗生素（硫酸慶大霉素）后C57BL/6J小鼠內(nèi)毛細(xì)胞傳入神經(jīng)突觸的形態(tài)和功能變化，并進(jìn)一步研究這種變化和聽力改變的關(guān)系。 方法 選擇出生后5周大小的C57BL/6J小鼠，雌雄不限，每組由6只小鼠組成，每天每只小鼠腹腔注射硫酸慶大霉素，藥物濃度為100mg/kg，連續(xù)給藥14d,然后停藥后飼養(yǎng)至第28d.分別選取第4d、第7d、第10d、第14d和第28d的小鼠進(jìn)行ABR（ClickTone burst）和DPOAE檢測,未進(jìn)行腹腔注射給藥的小鼠（0d）作為對照組，對照組動物每天給予相同劑量的生理鹽水。動物處死后耳蝸取材，然后分別對實(shí)驗(yàn)組和對照組小鼠進(jìn)行基底膜鋪片染色和蛋白檢測，以觀察耳蝸內(nèi)毛細(xì)胞傳入神經(jīng)突觸前特異性蛋白RIBEYE／CtBP2和突觸后神經(jīng)遞質(zhì)GluR2/3受體的表達(dá)情況。 結(jié)果 應(yīng)用慶大霉素以后，，實(shí)驗(yàn)組小鼠從第4d開始出現(xiàn)聽力損失，在第7d時聽力損失似乎達(dá)到最明顯的水平，而第10d,14d,28d聽力閾值較第7d時有所降低，但并無顯著性差異（P＞0.05），而實(shí)驗(yàn)組小鼠在第10d,14d,28d的聽力閾值仍顯著高于對照組（P＜0.05），在應(yīng)用慶大霉素后的第4d,7d,10d,14d和28d, RIBEYE／CtBP2和GluR2/3在基底膜上的表達(dá)水平和部位均出現(xiàn)了明顯的變化，而且這種變化和小鼠聽閾的改變存在一定的對應(yīng)關(guān)系。 結(jié)論 一定劑量的慶大霉素所造成的聽力損害可能和內(nèi)毛細(xì)胞傳入神經(jīng)突觸的形態(tài)及功能改變有關(guān)，而且該突觸形態(tài)上的恢復(fù)可能有助于小鼠聽力的改善。
[Abstract]:Purpose. To observe the morphological and functional changes of afferent synapses in C57BL / 6J mice after intraperitoneal injection of aminoglycoside antibiotics (gentamicin sulfate), and to further study the relationship between this change and hearing changes. Method. Five weeks after birth, C57BL / 6J mice, male and female, were randomly divided into six groups. Each mouse was intraperitoneally injected with gentamicin sulfate every day. The drug concentration was 100 mg / kg for 14 days, and then was fed to 28 days after withdrawal. The mice of 4 days, 7 days, 10 days, 14 days and 28 days were tested for ABR(ClickTone burst and DPOAE, and the mice that were not injected intraperitoneally for 0 d) were used as control group. The rats in the control group were given the same dose of normal saline every day. The cochlea were collected after the animals were killed, and then the mice in the experimental group and the control group were stained with basement membrane and tested for protein. To observe the expression of presynaptic specific protein (RIBEYE/CtBP2) and postsynaptic neurotransmitter (GluR2/3) receptor of afferent nerve in cochlear hair cells. Results. After application of gentamicin, the hearing loss in the experimental group appeared from the 4th day, and appeared to reach the most obvious level on the 7th day, and the threshold of hearing loss on the 10th day after 14 days was lower than that on the seventh day. However, there was no significant difference (P > 0.05), but the hearing threshold of the experimental group was significantly higher than that of the control group on the 10th day, 14d and 28d, and the expression of RIBEYE/CtBP2 and GluR2/3 on the basement membrane showed obvious changes on the 4th day, 7th day, 10d, 14d and 28d after gentamicin administration, but the hearing threshold of the experimental group was significantly higher than that of the control group (P < 0.05). Moreover, there is a certain correspondence between the change of hearing threshold and the change of hearing threshold in mice. Conclusion. The hearing loss caused by gentamicin at a certain dose may be related to the morphological and functional changes of the afferent synapse of the inner hair cell, and the morphological recovery of the synapse may contribute to the improvement of the hearing ability of the mice.
【學(xué)位授予單位】：遼寧醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R764.35

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