本文關鍵詞： 水楊酸鈉 耳蝸螺旋神經(jīng)節(jié)神經(jīng)元 多巴胺受體 N-甲基-D-天冬氨酸受體 A型γ-氨基丁酸受體　出處：《臨床耳鼻咽喉頭頸外科雜志》2017年20期 　論文類型：期刊論文

【摘要】：目的:觀察水楊酸鈉(SS)對大鼠耳蝸螺旋神經(jīng)節(jié)神經(jīng)元(SGN)的多巴胺受體(DR)亞型表達的影響,以及觀察SS作用下,DR對SGN的N-甲基-D-天冬氨酸(NMDA)受體和γ-氨基丁酸(GABA)a受體亞單位的影響,探討SS介導的受體之間的相互作用。方法:應用免疫熒光技術,檢測新生SD大鼠SGN中DR兩家族DR1、DR2的表達;應用RT-PCR技術,檢測SS處理后的SGN中DR1和DR2亞型的mRNA變化,觀察在激活和抑制DR活動時SS對SGN的NMDA受體亞單位NR1、GABAa受體亞單位GABRα2的mRNA表達的影響。結(jié)果:免疫熒光顯示:在SGN的胞體與突起均有DR1、DR2的免疫熒光顯示。RT-PCR結(jié)果顯示:(1)SGN有DR亞型(DRd1~DRd5)、GABRα2、NR1的表達;(2)5mmol/L SS作用SGN 30min后,除DRd3外,其余DR亞型及GABRα2、NR1的mRNA表達量均明顯升高,其中DRd1表達增加34.64%(t=-5.123,P=0.007);DRd2表達增加34.60%(t=-5.206,P=0.006);DRd4表達增加20.87%(t=-3.337,P=0.029);DRd5表達增加26.42%(t=-6.054,P=0.004);GABRα2表達增加30.41%(t=-2.839,P=0.047);NR1表達增加39.22%(t=-6.243,P=0.003);(3)SS分別與多巴胺(100μmol/L)、DR1激動劑(SKF38393,20μmol/L)、DR2激動劑(Quinpirole,20μmol/L)分別作用SGN 30min后,GABRα2、NR1的mRNA表達量均明顯升高,其中,GABRα2表達分別增加21.78%、27.45%、33.02%、33.42%(F=12.399,P=0.001),NR1表達分別增加28.70%、26.82%、29.03%、35.05%(F=50.395,P=0.000);(4)SS+DR1拮抗劑(SCH23390,20μmol/L)、SS+DR2拮抗劑(Eticlopride,20μmol/L)分別作用SGN 30min后,與單獨SS作用相比,GABRα2的mRNA表達分別減少29.56%、37.10%(F=22.101,P=0.000),NR1的mRNA表達分別減少37.62%、32.83%(F=72.933,P=0.000)。結(jié)論:SS可誘導SGN的大部分DR亞型的mRNA表達明顯升高;SS可能通過DR影響SGN上GABAaR、NMDAR的mRNA表達。
[Abstract]:Aim: to observe the effect of sodium salicylate (SSSS) on the expression of dopamine receptor (DRN) subtype in rat cochlear spiral ganglion neurons (SGNN). The effects of SS-induced Dr on the NMDA-NMDA-receptor and GABA receptor subunit of SGN were observed, and the interaction between the receptors mediated by SS-mediated was investigated. Methods: immunofluorescence technique was used. The expression of DR1DR2 in SGN of newborn SD rats was detected, and the mRNA changes of DR1 and DR2 subtypes in SGN treated with SS were detected by RT-PCR technique. To observe the effect of SS on the mRNA expression of SGN NMDA receptor subunit NR1GABAa receptor subunit GABR 偽 2 during activation and inhibition of Dr activity. Results: immunofluorescence showed that DR1 DR2 was present in the cell bodies and processes of SGN. RT-PCR showed that. The expression of GABR 偽 2NR1 in the SGN of Dr subtype DRd1, DRd5, and the expression of GABR 偽 2NR1 in SGN were detected after 30 minutes of SGN treatment with 5 mmol / L SS. With the exception of DRd3, all the Dr subtypes and the mRNA expression of GABR 偽 2nr 1 were significantly increased. The expression of DRd1 was increased by 34.64t-5.123P0. 007DRd2 and the expression of DRd4 was increased by 34.60t-5.206P0. 006DRd4. The expression of DRd5 was increased by 20.87t- 3.337P0. 029Dd5. The expression of GABR 偽 2 was increased by 26.42t-6.054P0.004 P0. 007. The expression of NR1 was increased by 39.22t-6.243P0. 243P0. 003SS and the expression of F3938320DR2 agonist Quinle20 / L SGN was significantly increased after the expression of NR1 was significantly increased, and the expression of NR1 was increased by 39.22t-6.243P0. 003SS, respectively. The expression of GABR 偽 2 was increased by 21.78 + 27.45 and 33.022, respectively, and the expression of NR1 was increased by 28.70,26.82and 29.035.050.395P0.0004SS DR1 antagonist, Eticlopride20 渭 mol / L, 20 渭 mol / L, respectively, after 30 minutes of treatment with Eticlopride20 渭 mol / L, a DR2 antagonist, Eticlopridea, a 20 渭 mol / L DR2 antagonist of Sch 23390,20 渭 mol / L, respectively. Compared with SS alone, the mRNA expression of GABR 偽 2 was reduced by 29.56% and 37.10%, respectively. The mRNA expression of NR1 was decreased by 37.62%, 32.833%, respectively. Conclusion the mRNA expression of most Dr subtypes of SGN can be induced by VSS to increase the mRNA expression of GABARARN NMDAR on SGN by the influence of VSS on the mRNA expression of GABARARN NMDAR on SGN. [WT5 "HZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ] [WT5 "BZ] [WT5" BZ] [WT5BZ] [WT5BZ]
【作者單位】： 廣西醫(yī)科大學第一附屬醫(yī)院耳鼻咽喉頭頸外科;欽州市第二人民醫(yī)院耳鼻咽喉頭頸外科;
【基金】：國家自然科學基金(No:81560174,81360157) 廣西自然科學基金(No:2014GXNSFAA118137) 2017廣西研究生教育創(chuàng)新計劃項目(No:YCSW2017103)
【分類號】：R764

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