本文關(guān)鍵詞： 多重聚合酶鏈反應(yīng) 急性結(jié)膜炎 細(xì)菌培養(yǎng)　出處：《延邊大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討多重聚合酶鏈反應(yīng)技術(shù)(multiplex PCR,MPCR)對(duì)急性感染性結(jié)膜炎病因診斷中的應(yīng)用價(jià)值。 方法：通過(guò)建立標(biāo)準(zhǔn)細(xì)菌株和致病性腺病毒株的MPCR體系,對(duì)根據(jù)臨床表現(xiàn)擬診為典型急性細(xì)菌性結(jié)膜炎組38眼、典型急性病毒性結(jié)膜炎組35眼、非典型急性結(jié)膜炎組30眼、正常對(duì)照組28眼等共131眼(131例)的結(jié)膜囊分泌物進(jìn)行MPCR檢測(cè)及細(xì)菌培養(yǎng),并對(duì)其結(jié)果進(jìn)行對(duì)比分析。 結(jié)果：(1)在本次MPCR體系中標(biāo)準(zhǔn)表皮葡萄球菌、金黃色葡萄球菌、大腸桿菌、肺炎鏈球菌DNA均擴(kuò)增出520bp大小特異性條帶。(2)典型急性細(xì)菌性結(jié)膜炎組(Ⅰ組)中擴(kuò)增出520bp條帶的有25眼(陽(yáng)性率65.8%),明顯高于典型急性病毒性結(jié)膜炎組(Ⅱ組)7眼(陽(yáng)性率20.0%)(χ2=15.516,P0.001);Ⅱ組1004bp條帶的有25眼(陽(yáng)性率60.0%)明顯高于Ⅰ組的6眼(陽(yáng)性率17.1%)(χ2=17.071,P0.001)。(3)Ⅰ組和非典型急性結(jié)膜炎組(Ⅲ組)共68眼中擴(kuò)增出520bp條帶的有40眼(陽(yáng)性率58.8%),細(xì)菌培養(yǎng)陽(yáng)性率為67.6%,兩種檢測(cè)方法,差異無(wú)統(tǒng)計(jì)學(xué)意義(χ2=1.139,P=0.286)。(4)Ⅰ組、Ⅱ組、Ⅲ組與Ⅳ組(正常對(duì)照組)各比較MPCR陽(yáng)性率后,結(jié)果均有顯著統(tǒng)計(jì)學(xué)差異(P0.001),Ⅰ組、Ⅱ組、Ⅲ組的MPCR陽(yáng)性檢測(cè)率明顯高于Ⅳ組。(5)Ⅰ組、Ⅲ組、Ⅳ組的MPCR細(xì)菌檢出率與細(xì)菌培養(yǎng)陽(yáng)性率比較結(jié)果,只有Ⅳ組的兩種檢測(cè)方法之間存在統(tǒng)計(jì)學(xué)差異(χ2=10.308,P=0.036)正常對(duì)照組的細(xì)菌培養(yǎng)檢測(cè)率高于MPCR檢測(cè)率。 結(jié)論：(1)急性細(xì)菌和病毒性結(jié)膜炎的MPCR檢測(cè)率具有顯著性差異,說(shuō)明MPCR檢測(cè)具有良好的特異性。(2)MPCR檢測(cè)法對(duì)急性感染性結(jié)膜炎進(jìn)行病因診斷具有臨床應(yīng)用意義。此方法與細(xì)菌培養(yǎng)法相比有速度快、特異性強(qiáng)、靈敏度高、經(jīng)濟(jì)簡(jiǎn)便等優(yōu)勢(shì)。
[Abstract]:Objective: to evaluate the value of multiplex PCR technique in the etiological diagnosis of acute infectious conjunctivitis. Methods: by establishing MPCR system of standard bacterial strain and pathogenic adenovirus strain, 38 eyes of typical acute bacterial conjunctivitis group and 35 eyes of typical acute viral conjunctivitis group were diagnosed according to clinical manifestations. The secretion of conjunctival sac in 30 eyes of atypical acute conjunctivitis group and 28 eyes of normal control group (131 eyes) were detected by MPCR and bacterial culture, and the results were compared and analyzed. Results in this MPCR system, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli were standard. 520bp size-specific bands were amplified from Streptococcus pneumoniae DNA. 520bp bands were amplified from typical acute bacterial conjunctivitis group (group 鈪，

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