不同免疫學方法對兔小關節(jié)異體移植的影響研究
發(fā)布時間:2019-03-09 15:47
【摘要】:目的:本研究采用多種免疫學處理方法,對比研究兔同種異體小關節(jié)移植的實驗效果,旨在探討一種減少同種異體小關節(jié)移植免疫排斥反應,促進骨折愈合及避免關節(jié)退變的有效措施,為臨床上行同種異體小關節(jié)移植術提供新途徑。方法:隨機選取48只成年雄性新西蘭兔,以新西蘭兔上肢第三掌指關節(jié)為研究對象,術前1月處死18只新西蘭兔取第三掌指關節(jié)進行深低溫保存,余下30只隨機分A、B、C、D、E組,每組實驗動物為6只。A組為同種異體關節(jié)直接移植組,B組為同種異體關節(jié)直接移植+FK560免疫藥物組,C組為冷凍處理的同種異體關節(jié)移植組,D組為冷凍處理后的同種異體關節(jié)移植+FK560免疫藥物組,E組為冷凍處理后的同種異體關節(jié)移+FK560+MMF+Pred藥物組。異體關節(jié)移植術后分別于4周將實驗各組處死1只新西蘭兔觀察其大體標本,8周抽取各實驗動物組血清進行IL-2及IL-6含量檢測,12周進行影像學檢查,48周將實驗動物全部處死進行常規(guī)HE染色切片來觀察骨折的愈合以及關節(jié)面軟骨的情況。對比各組的實驗結果,實驗數(shù)據(jù)應用SPSS19.0統(tǒng)計軟件分析處理。結果:1)大體結果:術后4周大體標本除A實驗組3只新西蘭兔創(chuàng)口出現(xiàn)不同程度的紅腫、潰爛,其余4組創(chuàng)口均順利愈合。2)術后8周取兔血清觀察白介素-2結果:方差分析顯示各組間總差異有統(tǒng)計學意義(F=52.163,P0.001)。B、C組與A組比較P0.001,D、E組與B、C組比較P0.001。B與C組之間無差異(P=0.150),D與E組之間無差異(P=0.721)。結果顯示AB、CD、E。術后8周白介素-6結果:方差分析顯示各組間總差異有統(tǒng)計學意義(F=482.864,P0.001).B、C組與A組比較P0.001,D、E組與B、C組比較P0.001。但B與C組之間無差異(P=0.164),D與E組之間無差異(P=0.378)?偨Y果顯示AB、CD、E。3)術后12周X線顯示:骨折端骨痂生長情況及皮質骨塑形情況,總體相比B、C、D組結果均明顯優(yōu)于A.B組。4)術后48周組織學觀察關節(jié)軟骨面Wakitani score評分:A組得分遠高于其它組,B、C組間以及D、E組間差異不大。總體得分為:AB、CD、E。結論:同種異體小關節(jié)移植時,供體關節(jié)術前經深低溫冷凍處理,術后短期使用免疫抑制藥抗排斥,不僅可以有效的減少免疫排斥反應,也可以促進自體骨與供體骨更好的愈合,而且能延緩移植關節(jié)遠期的關節(jié)退變,從而最大程度的保存異體移植關節(jié)的功能。
[Abstract]:Objective: to compare the experimental results of rabbit facet allograft transplantation with various immunologic methods in order to explore a method to reduce the immune rejection of allogeneic facet transplantation. The effective measures to promote fracture healing and to avoid joint degeneration provide a new way for clinical transplantation of allogeneic facet. Methods: forty-eight adult male New Zealand rabbits were randomly selected. The third metacarpophalangeal joints of the upper limbs of New Zealand rabbits were killed 1 month before operation for cryopreservation. The remaining 30 rabbits were randomly divided into A, B, C, A, B, C. Group D, Group E, 6 animals in each group. Group A: direct transplantation of joints, Group B: direct transplantation of FK560 immunopharmaceuticals, Group C: cryopreserved allografts, Group A: allografts, Group B: allografts, Group C: cryopreserved allografts. Group D was FK560 immunopharmaceutical group after cryopreservation and group E was cryopreserved allogeneic joint transplantation with FK560 MMF Pred drug group. At 4 weeks after allogeneic joint transplantation, 1 New Zealand rabbit was sacrificed to observe the gross specimen, 8 weeks after transplantation, the serum levels of IL-2 and IL-6 were measured, and imaging examination was carried out at 12 weeks. All the animals were killed at 48 weeks and routine HE staining sections were used to observe the healing of fracture and articular cartilage. Comparing the experimental results of each group, the experimental data were analyzed and processed by SPSS19.0 statistical software. Results: 1) Gross results: 4 weeks after operation, except for 3 New Zealand rabbits in experimental group A, there were different degrees of redness and ulceration in the wound. The results of ANOVA showed that there was a significant difference among the four groups (F = 52.163, P 0.001). B, C group compared with A group P 0.001, D, P < 0.01, P < 0.01, There was no difference between P0.001.B and C (P = 0.150), D vs E, P = 0.721). Results show AB,CD,E. Results of IL-6 at 8 weeks after operation: analysis of variance showed that there was a significant difference among the three groups (F = 482.864, P 0.001). B, C group compared with A group P0.001, D, E group compared with B, C group P 0.001. But there was no difference between group B and C (P = 0.164), D vs group E, P = 0.378). The results showed that 12 weeks after AB,CD,E.3, the growth of callus at fracture end and the shape of cortical bone were observed by X-ray, as compared with those of B, C, C, The results of group D were significantly better than those of group A. 4) the Wakitani score scores of articular cartilage surface in group A were significantly higher than those in other groups at 48 weeks after operation: group B, group C and group D, E had little difference. The overall score is: AB,CD,E. Conclusion: during allogeneic facet transplantation, cryopreservation of donor joints before operation and short-term use of immunosuppressive drugs against rejection after transplantation can not only effectively reduce the immune rejection, but also decrease the rejection of the donor joints. It can also promote the healing of autogenous bone and donor bone, and delay the long-term joint degeneration of transplanted joint, thus preserving the function of allogeneic joint to the maximum extent.
【學位授予單位】:南華大學
【學位級別】:碩士
【學位授予年份】:2015
【分類號】:R687.4
本文編號:2437615
[Abstract]:Objective: to compare the experimental results of rabbit facet allograft transplantation with various immunologic methods in order to explore a method to reduce the immune rejection of allogeneic facet transplantation. The effective measures to promote fracture healing and to avoid joint degeneration provide a new way for clinical transplantation of allogeneic facet. Methods: forty-eight adult male New Zealand rabbits were randomly selected. The third metacarpophalangeal joints of the upper limbs of New Zealand rabbits were killed 1 month before operation for cryopreservation. The remaining 30 rabbits were randomly divided into A, B, C, A, B, C. Group D, Group E, 6 animals in each group. Group A: direct transplantation of joints, Group B: direct transplantation of FK560 immunopharmaceuticals, Group C: cryopreserved allografts, Group A: allografts, Group B: allografts, Group C: cryopreserved allografts. Group D was FK560 immunopharmaceutical group after cryopreservation and group E was cryopreserved allogeneic joint transplantation with FK560 MMF Pred drug group. At 4 weeks after allogeneic joint transplantation, 1 New Zealand rabbit was sacrificed to observe the gross specimen, 8 weeks after transplantation, the serum levels of IL-2 and IL-6 were measured, and imaging examination was carried out at 12 weeks. All the animals were killed at 48 weeks and routine HE staining sections were used to observe the healing of fracture and articular cartilage. Comparing the experimental results of each group, the experimental data were analyzed and processed by SPSS19.0 statistical software. Results: 1) Gross results: 4 weeks after operation, except for 3 New Zealand rabbits in experimental group A, there were different degrees of redness and ulceration in the wound. The results of ANOVA showed that there was a significant difference among the four groups (F = 52.163, P 0.001). B, C group compared with A group P 0.001, D, P < 0.01, P < 0.01, There was no difference between P0.001.B and C (P = 0.150), D vs E, P = 0.721). Results show AB,CD,E. Results of IL-6 at 8 weeks after operation: analysis of variance showed that there was a significant difference among the three groups (F = 482.864, P 0.001). B, C group compared with A group P0.001, D, E group compared with B, C group P 0.001. But there was no difference between group B and C (P = 0.164), D vs group E, P = 0.378). The results showed that 12 weeks after AB,CD,E.3, the growth of callus at fracture end and the shape of cortical bone were observed by X-ray, as compared with those of B, C, C, The results of group D were significantly better than those of group A. 4) the Wakitani score scores of articular cartilage surface in group A were significantly higher than those in other groups at 48 weeks after operation: group B, group C and group D, E had little difference. The overall score is: AB,CD,E. Conclusion: during allogeneic facet transplantation, cryopreservation of donor joints before operation and short-term use of immunosuppressive drugs against rejection after transplantation can not only effectively reduce the immune rejection, but also decrease the rejection of the donor joints. It can also promote the healing of autogenous bone and donor bone, and delay the long-term joint degeneration of transplanted joint, thus preserving the function of allogeneic joint to the maximum extent.
【學位授予單位】:南華大學
【學位級別】:碩士
【學位授予年份】:2015
【分類號】:R687.4
【參考文獻】
相關期刊論文 前1條
1 陸軍,吳蘇稼,施鑫;大塊骨關節(jié)軟骨冷凍保存的實驗研究[J];醫(yī)學研究生學報;2002年03期
,本文編號:2437615
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