【摘要】：背景:國內(nèi)外學(xué)者對(duì)椎間盤退變動(dòng)物模型的建立做了大量工作,通過改變椎間盤力學(xué)特性、纖維環(huán)損傷、酶化學(xué)法等多種建模方式模擬椎間盤退變的病理表現(xiàn)。目的:通過髓核抽吸法和纖維環(huán)薄層切除法建立椎間盤退變模型,比較兩種方法的造模效果。方法:取6月齡新西蘭大白兔20只,通過手術(shù)暴露L3-4、L4-5及L5-6椎間盤,隨機(jī)使用髓核抽吸法和纖維環(huán)薄層切除法對(duì)L3-4、L4-5椎間盤進(jìn)行干預(yù):(1)髓核抽吸組——利用16 G針頭在椎間盤纖維環(huán)穿刺并負(fù)壓抽吸10 s;(2)纖維環(huán)薄層切除組——在顯微鏡下輔助下,在兔椎間盤纖維環(huán)上切除1 mm×2 mm范圍的約1/2層纖維環(huán)組織(避免切透全層)。于造模第1,3,5,9,13周進(jìn)行核磁共振、X射線檢查及椎間盤Masson染色病理學(xué)觀察。結(jié)果與結(jié)論:(1)X射線檢查:纖維環(huán)薄層切除組椎間隙高度自術(shù)后第1周起便發(fā)生不同程度降低;髓核抽吸組椎間隙在術(shù)后1周及3周輕微降低,自第5周起發(fā)生較明顯降低;纖維環(huán)薄層切除組術(shù)后第3,5,9周椎間隙高度指數(shù)百分比高于髓核抽吸組(P0.05);(2)核磁共振檢查:兩種方法均誘導(dǎo)相應(yīng)椎間盤發(fā)生退變,各組隨時(shí)間推移椎間盤退變程度逐漸加重,并于9周后均達(dá)到4級(jí)退變;髓核抽吸組術(shù)后1,3,5周的椎間盤退變程度重于纖維環(huán)薄層切除組(P0.05);(3)病理學(xué)觀察:隨時(shí)間推移,兩組椎間盤髓核及纖維環(huán)退變程度逐漸加重;(4)結(jié)果表明:髓核抽吸法和纖維環(huán)薄層切除法均能誘導(dǎo)椎間盤退變,纖維環(huán)薄層切除法在5周內(nèi)造成的椎間盤退變程度較髓核抽吸法輕,纖維環(huán)薄層切除法在纖維環(huán)上所造成的標(biāo)示部位可作為纖維環(huán)修補(bǔ)研究的靶點(diǎn)。
[Abstract]:Background: scholars at home and abroad have done a lot of work on the establishment of animal model of intervertebral disc degeneration. By changing the mechanical properties of intervertebral disc, the damage of fibrous ring, enzyme chemical method and other modeling methods to simulate the pathological manifestations of disc degeneration. Objective: to establish the model of disc degeneration by the method of nucleus pulposus aspiration and thin-layer excision of fibrous ring, and compare the effect of the two methods. Methods: twenty New Zealand white rabbits aged 6 months were exposed to L3-4 and L4-5 and L5-6 intervertebral discs by surgery. L3-4 was randomly treated by nucleus pulposus aspiration and thin-layer annular excision. L4-5 intervertebral disc intervention: (1) Nucleus pulposus aspiration group-16G needle in disc fiber ring puncture and suction negative pressure for 10 seconds; (2) Thin-layer excision of the fibrous annulus: with the aid of microscope, about 1 / 2 layer of fibrous annular tissue in the range of 1 mm 脳 2 mm was removed on the rabbit intervertebral disc annulus (avoiding cutting through the whole layer). Nuclear magnetic resonance (MRI), X-ray examination and Masson staining of intervertebral disc were performed at 913 weeks. Results and conclusion: (1) X-ray examination: the height of intervertebral space in thin layer excision group decreased in varying degrees from the first week after operation. The intervertebral space decreased slightly at 1 and 3 weeks postoperatively in the nucleus pulposus aspiration group and decreased significantly at the 5th week, and the percentage of the intervertebral space height index in the thin-layer excision group was higher than that in the nucleus pulposus suction group at the 9th week after operation (P0.05). (2) MRI: the degeneration of the intervertebral disc was induced by both methods, and the degree of degeneration of the intervertebral disc increased gradually with the passage of time, and reached grade 4 after 9 weeks. The degenerative degree of intervertebral disc in the pulposus aspiration group was more serious than that in the thin-layer excision group (P0.05); (3). The degeneration degree of the nucleus pulposus and the fibrous ring of the intervertebral disc increased gradually with the passage of time. (4) the results showed that the disc degeneration could be induced by the pulposus aspiration method and the thin-layer excision method, and the degree of disc degeneration caused by the thin-layer excision of the fibrous ring in 5 weeks was less than that in the nucleus pulposus aspiration method. The marking site caused by thin-layer excision of fiber ring on the fiber ring can be used as a target for the repair of fiber ring.
【作者單位】： 新疆醫(yī)科大學(xué)第六附屬醫(yī)院脊柱外科;
【分類號(hào)】：R681.53;R-332

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張勇;徐永清;王非;陸華拓;唐輝;游永剛;;椎間盤退變模型及其評(píng)估指標(biāo)[J];中國脊柱脊髓雜志;2009年06期

2 席志鵬;周臨東;謝林;;椎間盤退變模型研究進(jìn)展[J];山西中醫(yī)學(xué)院學(xué)報(bào);2010年02期

3 武海軍;銀和平;;椎間盤退變的分子生物學(xué)研究進(jìn)展[J];內(nèi)蒙古醫(yī)學(xué)雜志;2011年07期

4 楊松波;高春華;龐曉東;李端明;楊洪;彭寶淦;;椎間盤退變模型的研究進(jìn)展[J];脊柱外科雜志;2012年05期

5 彭寶淦,賈連順;椎間盤退變機(jī)理的研究進(jìn)展[J];中國矯形外科雜志;2000年04期

6 黃宗強(qiáng),劉尚禮,鄭召民;椎間盤退變的分子生物學(xué)研究進(jìn)展[J];中國矯形外科雜志;2003年01期

7 劉彭華;椎間盤退變的性別差異分析(附100例報(bào)告)[J];現(xiàn)代醫(yī)用影像學(xué);2003年04期

8 張銀剛,郭雄;椎間盤退變的發(fā)生機(jī)制及其修復(fù)的生物學(xué)進(jìn)展[J];中華骨科雜志;2004年02期

9 趙勇,王文波;基因治療與椎間盤退變[J];中國矯形外科雜志;2004年17期

10 賀治青,葉曉健;椎間盤退變干預(yù)與修復(fù)的研究進(jìn)展[J];脊柱外科雜志;2004年04期

相關(guān)會(huì)議論文 前10條

1 夏冬冬;林勝磊;王向陽;;兔椎間盤退變模型的研究進(jìn)展[A];2012年浙江省骨科學(xué)術(shù)年會(huì)論文集[C];2012年

2 夏冬冬;林勝磊;王向陽;;兔椎間盤退變模型的研究進(jìn)展[A];2013中國工程院科技論壇暨浙江省骨科學(xué)學(xué)術(shù)年會(huì)論文摘要集[C];2013年

3 張寧;陳維善;陳其昕;徐侃;李方財(cái);吳瓊?cè)A;;酒精與椎間盤退變[A];2008年浙江省骨科學(xué)學(xué)術(shù)年會(huì)論文匯編[C];2008年

4 劉q，

本文編號(hào)：2331870