【摘要】：目的近年來,干細胞移植逐步成為椎間盤退變性疾病治療的研究熱門。退變椎間盤中惡劣的微環(huán)境對移植干細胞的生長、增殖、分化都有影響。近期有研究表明,"預(yù)分化"處理可以增強移植細胞對微環(huán)境的適應(yīng)能力。本研究旨在探究預(yù)分化處理對干細胞在椎間盤微環(huán)境中生物學(xué)行為的影響。方法將大鼠脂肪干細胞(ADSC,adipose-derived stem cell)分為5組,予Ⅱ型膠原(typeⅡcollagen)包埋處理后,分別進行成軟骨分化處理0天(對照組),3,7,10,14天(實驗組);再將各組細胞移植于模擬退變椎間盤的微環(huán)境(酸性,低糖,高滲透壓)培養(yǎng)7天;用細胞計數(shù)試劑盒(CCK-8)檢測各組細胞的增殖情況,用聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)、蛋白印記及免疫熒光法檢測干細胞的細胞外基質(zhì)合成情況。結(jié)果一周的分化過程中,預(yù)分化7天的ADSC增殖能力相對最強。功能基因合成方面,D7組和D10組Ⅱ型膠原mRNA的表達量均較對照組增高;D7組的多聚蛋白聚糖mRNA表達量較對照組高。結(jié)論通過本次研究我們得出結(jié)論,成軟骨細胞預(yù)分化處理7至10天的可能促進脂肪干細胞在椎間盤惡劣環(huán)境中的增殖能力和細胞外基質(zhì)合成能力,有利于干細胞對退變椎間盤的再生。
[Abstract]:Objective in recent years, stem cell transplantation has gradually become a hot topic in the treatment of degenerative disc diseases. The poor microenvironment in degenerative intervertebral disc affects the growth, proliferation and differentiation of stem cells. Recent studies have shown that pre-differentiation can enhance the ability of transplanted cells to adapt to microenvironment. The aim of this study was to investigate the effects of pre-differentiation on the biological behavior of stem cells in intervertebral disc microenvironment. Methods Rat adipose-derived stem cell) were divided into 5 groups, and were treated with type 鈪，

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